##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487461
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.888879315473442	33.0	33.0	33.0	33.0	33.0
2	32.01453244464685	33.0	33.0	33.0	33.0	33.0
3	31.72175825348079	33.0	33.0	33.0	27.0	33.0
4	32.005235290618124	33.0	33.0	33.0	33.0	33.0
5	32.099406926913126	33.0	33.0	33.0	33.0	33.0
6	35.27254693195969	37.0	37.0	37.0	33.0	37.0
7	35.41027282182574	37.0	37.0	37.0	33.0	37.0
8	35.551170247465954	37.0	37.0	37.0	33.0	37.0
9	35.65708231017456	37.0	37.0	37.0	33.0	37.0
10	35.67305076713829	37.0	37.0	37.0	33.0	37.0
11	35.68770219566283	37.0	37.0	37.0	33.0	37.0
12	35.68247305938321	37.0	37.0	37.0	33.0	37.0
13	35.694590951891534	37.0	37.0	37.0	33.0	37.0
14	35.656981789312375	37.0	37.0	37.0	33.0	37.0
15	35.6801877483532	37.0	37.0	37.0	33.0	37.0
16	35.65507599582325	37.0	37.0	37.0	33.0	37.0
17	35.657964432026354	37.0	37.0	37.0	33.0	37.0
18	35.64442488732432	37.0	37.0	37.0	33.0	37.0
19	35.62927290593504	37.0	37.0	37.0	33.0	37.0
20	35.644402321416486	37.0	37.0	37.0	33.0	37.0
21	35.626501402163456	37.0	37.0	37.0	33.0	37.0
22	35.53374526372366	37.0	37.0	37.0	33.0	37.0
23	35.571245699655975	37.0	37.0	37.0	33.0	37.0
24	35.562555773692665	37.0	37.0	37.0	33.0	37.0
25	35.59225661129813	37.0	37.0	37.0	33.0	37.0
26	35.47235368573076	37.0	37.0	37.0	33.0	37.0
27	35.495709400341774	37.0	37.0	37.0	33.0	37.0
28	35.5202877768683	37.0	37.0	37.0	33.0	37.0
29	35.5231454413789	37.0	37.0	37.0	33.0	37.0
30	35.51553867899175	37.0	37.0	37.0	33.0	37.0
31	35.504544978982935	37.0	37.0	37.0	33.0	37.0
32	35.493674365744134	37.0	37.0	37.0	33.0	37.0
33	35.48071127741501	37.0	37.0	37.0	33.0	37.0
34	35.4604552979623	37.0	37.0	37.0	33.0	37.0
35	35.402778888977785	37.0	37.0	37.0	33.0	37.0
36	35.418343211046626	37.0	37.0	37.0	33.0	37.0
37	35.4080080252574	37.0	37.0	37.0	33.0	37.0
38	35.39174826293796	37.0	37.0	37.0	33.0	37.0
39	35.278346370273724	37.0	37.0	37.0	33.0	37.0
40	35.07927813712276	37.0	37.0	37.0	33.0	37.0
41	35.23327404653911	37.0	37.0	37.0	33.0	37.0
42	35.25443471375146	37.0	37.0	37.0	33.0	37.0
43	34.92209428036294	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	27.0
17	21.0
18	22.0
19	15.0
20	40.0
21	89.0
22	215.0
23	495.0
24	973.0
25	1853.0
26	3092.0
27	4494.0
28	6688.0
29	8830.0
30	11683.0
31	15075.0
32	19259.0
33	25616.0
34	37112.0
35	75108.0
36	276735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63032734926487	21.838875315153416	14.274988152898386	23.255809182683333
2	15.213729918906333	23.00142985797838	39.942477449478005	21.842362773637277
3	17.46252520714478	28.235079319166047	31.73853908312665	22.563856390562528
4	12.004857824523398	18.43593641337461	41.094774761468095	28.4644310006339
5	12.837744968315414	38.23793082933814	35.84389315247784	13.080431049868604
6	27.27643852533844	41.55081124438673	17.98892629359067	13.183823936684167
7	24.268197866085696	33.85275950281151	24.456110334980643	17.422932296122152
8	23.674304200746317	36.048422335325284	21.642962206207265	18.63431125772113
9	25.4775253815177	15.291890017868095	21.420175152473735	37.81040944814046
10	14.007069283491397	28.827536972188543	35.05265036587543	22.112743378444634
11	32.01712547260191	24.88793975312897	25.166731287220927	17.928203487048194
12	21.53772301784143	26.879278547412	31.933221324372617	19.649777110373957
13	27.52056061920851	23.319814303092965	27.38701147373841	21.77261360396011
14	21.157590043100885	22.455540032946224	29.728326984107444	26.658542939845443
15	23.026047211982085	29.764022147412817	26.15573348431977	21.054197156285323
16	21.215030535776194	30.006503084349312	26.468579024783523	22.30988735509097
17	21.09707238117511	28.57664510596745	27.74580940834241	22.58047310451503
18	21.723378895952703	27.73534703289084	29.468203610134964	21.073070461021498
19	23.411103657523373	27.23294786659856	29.850182886425785	19.505765589452285
20	23.376844506534884	26.76685929746175	29.575904533901177	20.280391662102197
21	21.775485628593877	28.23220729453228	28.836768479939934	21.15553859693391
22	21.17215531088641	28.033422160952366	29.207259657695694	21.587162870465534
23	21.17892508323743	27.702729038835926	29.800948178418373	21.317397699508266
24	21.834156988969376	27.81761002418655	29.10530278319701	21.242930203647063
25	21.824104902751195	27.627440964507933	29.633345026576485	20.91510910616439
26	21.62183231068742	27.769811328496026	29.09073751541149	21.517618845405067
27	21.592906919733064	27.8467405597576	29.03083528733581	21.529517233173525
28	20.979319371190723	28.01926718240023	29.472921936319008	21.52849151009004
29	22.032736977932593	27.955672351224	28.813997427486505	21.197593243356906
30	21.617319129120073	28.111171970680733	29.225312383965075	21.04619651623412
31	21.81015506881576	27.720166331255218	29.218747756230755	21.250930843698264
32	20.66237093839302	27.756682073027385	29.46184412701734	22.119102861562258
33	21.075942485655265	27.705395918852993	29.99316868426397	21.225492911227768
34	21.007424183678282	27.749502011442967	29.227568974748745	22.015504830130002
35	21.31226908409083	28.475919099168962	29.017911176483864	21.19390064025635
36	21.862466946073635	28.08717005052712	28.88292601869688	21.167436984702366
37	22.04278906415077	27.413269984675697	28.737888774691715	21.80605217648181
38	21.841747339787183	28.03814048713641	28.81707459673697	21.303037576339438
39	21.44643366341102	27.743142528325343	29.144075115752848	21.666348692510788
40	21.51905485772195	28.045935982570914	29.201105319194763	21.23390384051237
41	21.45217771267855	27.583950305768052	29.36706731410308	21.59680466745032
42	21.21872313887675	27.94972315733977	29.181616580608498	21.649937123174983
43	21.290934043954284	27.6713419124812	29.190027509893103	21.847696533671414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	48.0
2	65.0
3	206.5
4	348.0
5	348.0
6	454.5
7	561.0
8	515.5
9	470.0
10	686.0
11	902.0
12	902.0
13	1437.0
14	1972.0
15	3562.0
16	5152.0
17	5913.5
18	6675.0
19	6675.0
20	6630.0
21	6585.0
22	7697.0
23	8809.0
24	10465.5
25	12122.0
26	12122.0
27	14068.5
28	16015.0
29	19037.0
30	22059.0
31	23328.0
32	24597.0
33	24597.0
34	26480.5
35	28364.0
36	29822.5
37	31281.0
38	32356.5
39	33432.0
40	33432.0
41	34487.5
42	35543.0
43	34495.0
44	33447.0
45	33447.5
46	33448.0
47	33448.0
48	32614.0
49	31780.0
50	31919.0
51	32058.0
52	31899.5
53	31741.0
54	31741.0
55	28767.5
56	25794.0
57	22909.5
58	20025.0
59	17388.5
60	14752.0
61	14752.0
62	12806.5
63	10861.0
64	8712.5
65	6564.0
66	5530.0
67	4496.0
68	4496.0
69	3661.5
70	2827.0
71	2395.0
72	1963.0
73	1577.5
74	1192.0
75	1192.0
76	886.0
77	580.0
78	430.0
79	280.0
80	257.5
81	235.0
82	235.0
83	197.5
84	160.0
85	142.5
86	125.0
87	109.0
88	93.0
89	93.0
90	67.5
91	42.0
92	26.0
93	10.0
94	7.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	487461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.74842804049528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57447537769842	56.206575225026256
2	6.068614160356476	7.615919978933179
3	1.384256793730247	2.6057981343284733
4	0.636239354420892	1.5969207738964202
5	0.415983756789998	1.305116341447604
6	0.27172133956341654	1.0230052153597213
7	0.19865580517474066	0.8725737647083703
8	0.14673106664294064	0.736571502123974
9	0.11837862650122151	0.6685265453890107
>10	0.9857594360174834	12.803976050873139
>50	0.13157431023150878	5.702102987427953
>100	0.06562144425875294	7.052814486301527
>500	9.942643069508021E-4	0.39949450457584573
>1k	9.942643069508021E-4	1.4106044896085173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3217	0.6599502319159891	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.47367891995462197	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1257	0.25786678318880896	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.14072920705451308	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	650	0.13334400085340162	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	585	0.12000960076806146	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	488	0.10011057294839996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.154338500926228E-4	0.0	0.0
2	0.0	0.0	6.154338500926228E-4	0.0	0.0
3	0.0	0.0	6.154338500926228E-4	0.0	0.0
4	0.0	0.0	6.154338500926228E-4	2.0514461669754092E-4	0.0
5	0.0	0.0	6.154338500926228E-4	2.0514461669754092E-4	0.0
6	0.0	0.0	0.0010257230834877047	2.0514461669754092E-4	0.0
7	0.0	0.0	0.00225659078367295	4.1028923339508184E-4	0.0
8	0.0	0.0	0.002872024633765573	4.1028923339508184E-4	0.0
9	0.0	0.0	0.0038977477172532776	0.0012308677001852456	0.0
10	0.0	0.0	0.004102892333950819	0.0014360123168827865	0.0
11	2.0514461669754092E-4	0.0	0.005538904650833605	0.0014360123168827865	0.0
12	2.0514461669754092E-4	0.0	0.005949193884228687	0.0014360123168827865	0.0
13	2.0514461669754092E-4	0.0	0.0065646277343213095	0.0014360123168827865	0.0
14	2.0514461669754092E-4	0.0	0.006974916967716392	0.0016411569335803274	0.0
15	2.0514461669754092E-4	0.0	0.007795495434506555	0.0018463015502778685	0.0
16	2.0514461669754092E-4	0.0	0.008410929284599179	0.0018463015502778685	0.0
17	2.0514461669754092E-4	0.0	0.00861607390129672	0.0018463015502778685	0.0
18	2.0514461669754092E-4	0.0	0.00861607390129672	0.0018463015502778685	0.0
19	4.1028923339508184E-4	0.0	0.00861607390129672	0.0020514461669754094	0.0
20	4.1028923339508184E-4	0.0	0.0090263631346918	0.002461735400370491	0.0
21	4.1028923339508184E-4	0.0	0.009231507751389341	0.0032823138671606547	0.0
22	4.1028923339508184E-4	0.0	0.009231507751389341	0.0038977477172532776	0.0
23	4.1028923339508184E-4	0.0	0.009231507751389341	0.004102892333950819	0.0
24	4.1028923339508184E-4	0.0	0.009231507751389341	0.005128615417438523	0.0
25	4.1028923339508184E-4	0.0	0.009231507751389341	0.005538904650833605	0.0
26	4.1028923339508184E-4	0.0	0.009231507751389341	0.005744049267531146	0.0
27	4.1028923339508184E-4	0.0	0.009231507751389341	0.006359483117623769	0.0
28	6.154338500926228E-4	0.0	0.009231507751389341	0.017027003185895898	0.0
29	6.154338500926228E-4	0.0	0.009231507751389341	0.03672088638885983	0.0
30	6.154338500926228E-4	0.0	0.009231507751389341	0.06503084349312048	0.0
31	6.154338500926228E-4	0.0	0.009231507751389341	0.1232919146352221	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGC	25	0.0054932777	29.599998	6
AGCGTCA	50	9.077072E-6	25.899998	37
GTATCAA	1460	0.0	24.328768	1
GGTATCA	530	0.0	23.037737	1
TTAACGG	90	3.814421E-9	22.61111	35
TCGCACC	60	3.7206224E-5	21.583332	28
TTCGCCG	60	3.7206224E-5	21.583332	24
GTCGCCA	80	6.94321E-7	20.8125	12
GCACCGC	90	9.450196E-8	20.555555	10
AACGGCC	100	1.2852979E-8	20.349998	37
GTATTAG	185	0.0	20.0	1
CTGAGCG	65	6.8917114E-5	19.923077	34
ACGGGTA	65	6.8917114E-5	19.923077	15
TTCGCAC	65	6.8917114E-5	19.923077	27
TCGCCAT	85	1.2422988E-6	19.588236	13
TTTCGCA	70	1.21737045E-4	18.5	26
GTCTAGG	60	9.226418E-4	18.5	1
CCCACGC	90	2.147046E-6	18.5	19
CTTTCGC	70	1.21737045E-4	18.5	25
TGAGCGT	70	1.21737045E-4	18.5	35
>>END_MODULE