FastQCFastQC Report
Fri 10 Feb 2017
ERR1633236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466263
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16030.3437973847377982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.24792874407791313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7050.15120221849042279No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4710.1010159502255165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG250.005493035529.5999987
TCGCGCG250.005493035529.5999989
AACGGAG556.248338E-726.9090925
TGTATCG358.86146E-426.428575
GGTATCA3300.025.2272721
ACGGGTC551.8995157E-523.54545416
CGGGTAC400.001929475123.12500217
GCCGTAT753.730911E-722.28
GTATCAA8600.021.7267441
GGACAGT705.0911985E-621.1428576
CGGGTCA450.003822449420.55555517
GCTATAC450.003822449420.5555553
GCTTCGC450.003822449420.55555522
ATCGCGC450.003822449420.5555558
TATAACT1101.7462298E-920.1818184
GAGTCAA759.246412E-619.73333429
TACGGGT1500.019.73333415
CTAAACA759.246412E-619.7333344
CGAACTA951.6707781E-719.47368429
GTCTAGA951.6707781E-719.4736841
Produced by FastQC (version 0.11.5)