##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633236.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466263
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80635178000399	33.0	33.0	33.0	27.0	33.0
2	31.934138029395427	33.0	33.0	33.0	33.0	33.0
3	31.638888352710808	33.0	33.0	33.0	27.0	33.0
4	31.932992753017075	33.0	33.0	33.0	33.0	33.0
5	32.02770324902469	33.0	33.0	33.0	33.0	33.0
6	35.19404928119967	37.0	37.0	37.0	33.0	37.0
7	35.313818595942635	37.0	37.0	37.0	33.0	37.0
8	35.4476314869505	37.0	37.0	37.0	33.0	37.0
9	35.57392930599254	37.0	37.0	37.0	33.0	37.0
10	35.59780209881548	37.0	37.0	37.0	33.0	37.0
11	35.602619122683976	37.0	37.0	37.0	33.0	37.0
12	35.59212504530705	37.0	37.0	37.0	33.0	37.0
13	35.596041290001565	37.0	37.0	37.0	33.0	37.0
14	35.580556467058294	37.0	37.0	37.0	33.0	37.0
15	35.590136896987325	37.0	37.0	37.0	33.0	37.0
16	35.55876833460944	37.0	37.0	37.0	33.0	37.0
17	35.564930093101964	37.0	37.0	37.0	33.0	37.0
18	35.54652417198019	37.0	37.0	37.0	33.0	37.0
19	35.53642043224532	37.0	37.0	37.0	33.0	37.0
20	35.53956886992963	37.0	37.0	37.0	33.0	37.0
21	35.535090710607534	37.0	37.0	37.0	33.0	37.0
22	35.43976468216436	37.0	37.0	37.0	33.0	37.0
23	35.49120775184821	37.0	37.0	37.0	33.0	37.0
24	35.474418943814975	37.0	37.0	37.0	33.0	37.0
25	35.506894177749466	37.0	37.0	37.0	33.0	37.0
26	35.392046977778634	37.0	37.0	37.0	33.0	37.0
27	35.41432624934854	37.0	37.0	37.0	33.0	37.0
28	35.41934487617503	37.0	37.0	37.0	33.0	37.0
29	35.443046092012445	37.0	37.0	37.0	33.0	37.0
30	35.43789234830986	37.0	37.0	37.0	33.0	37.0
31	35.43518357665094	37.0	37.0	37.0	33.0	37.0
32	35.421583955836084	37.0	37.0	37.0	33.0	37.0
33	35.42141023413824	37.0	37.0	37.0	33.0	37.0
34	35.38994301499368	37.0	37.0	37.0	33.0	37.0
35	35.35051247900863	37.0	37.0	37.0	33.0	37.0
36	35.38407722680118	37.0	37.0	37.0	33.0	37.0
37	35.384990874249084	37.0	37.0	37.0	33.0	37.0
38	35.36894842610287	37.0	37.0	37.0	33.0	37.0
39	35.280918708968976	37.0	37.0	37.0	33.0	37.0
40	35.073424226241414	37.0	37.0	37.0	33.0	37.0
41	35.238371905984394	37.0	37.0	37.0	33.0	37.0
42	35.28418296111851	37.0	37.0	37.0	33.0	37.0
43	34.96750760836695	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	20.0
17	26.0
18	16.0
19	13.0
20	49.0
21	86.0
22	221.0
23	579.0
24	1189.0
25	2013.0
26	3365.0
27	4918.0
28	6720.0
29	9047.0
30	11840.0
31	14922.0
32	18924.0
33	24515.0
34	35333.0
35	71773.0
36	260678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2902160368719	20.596744755642202	13.246601167152445	24.86643804033346
2	16.392894139144644	21.485942483105028	37.975563147837164	24.14560022991316
3	18.137832081893695	25.99219753658343	30.101037397348705	25.76893298417417
4	12.877925119514094	17.380319690818272	39.11783692894353	30.623918260724096
5	14.084111327727056	37.04969083971921	35.17714251398888	13.689055318564844
6	30.597967241664037	39.45841724520282	16.825268142657684	13.118347370475462
7	27.313554796327395	31.661744551894532	23.127290820845744	17.89740983093233
8	24.972386828892706	35.76179109215186	20.406723244177645	18.859098834777797
9	25.685074732500755	15.241612566298421	20.39642862504638	38.676884076154444
10	14.647956196395596	28.459260117144186	33.7539114190918	23.138872267368416
11	34.10307058462713	23.416398041448712	24.114930843751274	18.365600530172884
12	22.85298211524397	25.542451363286382	30.52762067760041	21.076945843869233
13	28.026457171167348	22.102976217285093	26.29417303110047	23.576393580447085
14	22.363987706508986	20.56800561056743	27.33628874690893	29.73171793601465
15	23.700786894949847	29.27425079836916	24.22087963231052	22.804082674370473
16	23.717301179806245	28.130261247407578	24.718023947857752	23.43441362492842
17	22.43776581028304	27.7240527341865	26.38360753480332	23.454573920727142
18	23.35591715405254	27.209536248855258	27.641052367440693	21.793494229651504
19	24.72402914235099	26.329131841900388	27.01415295659317	21.932686059155454
20	23.841480023077104	26.97533366361903	27.184657585954707	21.998528727349157
21	23.03120770895396	26.807831631504108	27.80941228448322	22.351548375058712
22	22.806227386689486	26.753141467369275	27.469260910687744	22.971370235253495
23	22.832821819445247	26.63003498025792	27.577139940334106	22.960003259962726
24	22.961290087354133	27.318702105893028	26.326772658349473	23.39323514840337
25	23.466369838481715	27.182727344867597	27.06562605224948	22.285276764401207
26	23.808236982132403	26.610947040618704	27.11130842464446	22.46950755260443
27	23.295650737888273	26.99956891282388	26.788529220632988	22.916251128654856
28	22.817594361980255	26.524944076626284	27.748287983391347	22.909173578002115
29	23.603202484434753	26.398406049804507	26.795392300053834	23.20299916570691
30	23.111205478453147	26.737270596208578	27.62217889903338	22.5293450263049
31	23.401813997679422	26.663492492434525	27.279024927991287	22.655668581894766
32	22.627143908051895	26.693089522436907	27.47462269148528	23.205143878025922
33	22.755612175960778	26.6583451828689	27.948389642755266	22.63765299841506
34	23.4970392246436	26.134820905797802	27.279668341686985	23.088471527871608
35	22.912819588944437	27.11302419449967	27.31612845111021	22.65802776544568
36	23.901960910473274	26.370095847193536	27.19473773385407	22.53320550847912
37	23.472803975438755	26.188438713773127	27.26701453900481	23.071742771783306
38	23.443206945436373	26.587569676341467	27.32964013871999	22.63958323950217
39	23.21522402592528	26.616523292648143	27.221117695377934	22.947134986048646
40	23.003326448806792	27.22390582139265	27.27516444581706	22.4976032839835
41	23.420687466086736	26.037236495282706	27.3963406918413	23.14573534678926
42	22.835395474228065	26.81791177940347	27.212109903638076	23.13458284273039
43	23.315167619991293	26.232190845080993	27.14562382174867	23.307017713179043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	36.0
2	47.0
3	119.5
4	192.0
5	192.0
6	223.5
7	255.0
8	232.5
9	210.0
10	294.0
11	378.0
12	378.0
13	607.0
14	836.0
15	1531.0
16	2226.0
17	2622.0
18	3018.0
19	3018.0
20	3145.5
21	3273.0
22	3787.5
23	4302.0
24	5365.0
25	6428.0
26	6428.0
27	8387.0
28	10346.0
29	11894.5
30	13443.0
31	15410.0
32	17377.0
33	17377.0
34	20451.5
35	23526.0
36	25775.5
37	28025.0
38	29668.5
39	31312.0
40	31312.0
41	32796.0
42	34280.0
43	35584.5
44	36889.0
45	36544.0
46	36199.0
47	36199.0
48	34978.5
49	33758.0
50	34321.5
51	34885.0
52	33538.0
53	32191.0
54	32191.0
55	30575.5
56	28960.0
57	24982.0
58	21004.0
59	20267.0
60	19530.0
61	19530.0
62	17938.0
63	16346.0
64	13226.5
65	10107.0
66	8385.5
67	6664.0
68	6664.0
69	5364.0
70	4064.0
71	3375.0
72	2686.0
73	2098.5
74	1511.0
75	1511.0
76	1168.5
77	826.0
78	627.5
79	429.0
80	348.5
81	268.0
82	268.0
83	219.0
84	170.0
85	150.0
86	130.0
87	110.0
88	90.0
89	90.0
90	65.5
91	41.0
92	27.5
93	14.0
94	8.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.59546358108838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14068438473872	49.88857871134898
2	8.062767931163807	9.448832493465522
3	2.318476218482321	4.075565664711011
4	1.1209751555957455	2.6273623562006154
5	0.6682039389357403	1.9576859784324485
6	0.4249311476336562	1.4939422551382797
7	0.31446968744400894	1.2898547982587172
8	0.23456849082589457	1.0995719569167657
9	0.19076524989645827	1.0060180427560632
>10	1.3442433257320818	15.655312015422073
>50	0.12649656520435124	5.05154786470018
>100	0.052305031339942694	5.652777699221538
>500	3.7095766907760776E-4	0.15324187794936495
>1k	7.419153381552155E-4	0.5997082854784367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1603	0.3437973847377982	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1156	0.24792874407791313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	705	0.15120221849042279	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	471	0.1010159502255165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.289424638026178E-4	0.0	0.0
2	0.0	0.0	4.289424638026178E-4	0.0	0.0
3	0.0	0.0	6.434136957039268E-4	0.0	0.0
4	0.0	0.0	6.434136957039268E-4	0.0	0.0
5	0.0	0.0	6.434136957039268E-4	0.0	0.0
6	0.0	0.0	8.578849276052356E-4	0.0	0.0
7	0.0	0.0	0.0012868273914078535	0.0	0.0
8	0.0	0.0	0.0012868273914078535	0.0	0.0
9	0.0	0.0	0.0012868273914078535	6.434136957039268E-4	0.0
10	0.0	0.0	0.0017157698552104713	8.578849276052356E-4	0.0
11	0.0	0.0	0.0034315397104209425	8.578849276052356E-4	0.0
12	0.0	0.0	0.003646010942322252	8.578849276052356E-4	0.0
13	0.0	0.0	0.0038604821742235606	8.578849276052356E-4	0.0
14	0.0	0.0	0.004503895869927488	8.578849276052356E-4	0.0
15	0.0	0.0	0.005147309565631414	8.578849276052356E-4	0.0
16	0.0	0.0	0.005147309565631414	8.578849276052356E-4	0.0
17	0.0	0.0	0.005361780797532723	8.578849276052356E-4	0.0
18	0.0	0.0	0.005790723261335341	8.578849276052356E-4	0.0
19	0.0	0.0	0.005790723261335341	8.578849276052356E-4	0.0
20	0.0	0.0	0.005790723261335341	0.0010723561595065447	0.0
21	0.0	0.0	0.005790723261335341	0.0012868273914078535	0.0
22	0.0	0.0	0.0062196657251379584	0.0012868273914078535	0.0
23	0.0	0.0	0.0062196657251379584	0.0019302410871117803	0.0
24	0.0	0.0	0.006434136957039267	0.0023591835509143982	0.0
25	0.0	0.0	0.006434136957039267	0.0023591835509143982	0.0
26	0.0	0.0	0.006434136957039267	0.003002597246618325	0.0
27	0.0	0.0	0.006434136957039267	0.004289424638026179	0.0
28	0.0	0.0	0.006434136957039267	0.014798515001190315	0.0
29	0.0	0.0	0.006434136957039267	0.030883857393788485	0.0
30	0.0	0.0	0.006434136957039267	0.04739814225018927	0.0
31	0.0	0.0	0.006434136957039267	0.09222262971756283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	25	0.0054930355	29.599998	7
TCGCGCG	25	0.0054930355	29.599998	9
AACGGAG	55	6.248338E-7	26.90909	25
TGTATCG	35	8.86146E-4	26.42857	5
GGTATCA	330	0.0	25.227272	1
ACGGGTC	55	1.8995157E-5	23.545454	16
CGGGTAC	40	0.0019294751	23.125002	17
GCCGTAT	75	3.730911E-7	22.2	8
GTATCAA	860	0.0	21.726744	1
GGACAGT	70	5.0911985E-6	21.142857	6
CGGGTCA	45	0.0038224494	20.555555	17
GCTATAC	45	0.0038224494	20.555555	3
GCTTCGC	45	0.0038224494	20.555555	22
ATCGCGC	45	0.0038224494	20.555555	8
TATAACT	110	1.7462298E-9	20.181818	4
GAGTCAA	75	9.246412E-6	19.733334	29
TACGGGT	150	0.0	19.733334	15
CTAAACA	75	9.246412E-6	19.733334	4
CGAACTA	95	1.6707781E-7	19.473684	29
GTCTAGA	95	1.6707781E-7	19.473684	1
>>END_MODULE