FastQCFastQC Report
Fri 10 Feb 2017
ERR1633235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590359
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57300.970595857774676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.7173601147776184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23020.3899322276784126No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19500.33030749086572747No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC18150.3074400491904079No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16850.28541954979935935No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG16660.28220116911912924No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG16360.27711951541350266No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA16310.27627257312923154No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14620.24764592392086848No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG14190.24036222027613705No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14130.23934588953501176No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13660.23138463206286344No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT13630.23087646669230077No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT13120.2222376553927356No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG12910.21868049779879703No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12790.21664783631654638No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12190.20648452890529323No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT11890.20140287519966665No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT11610.1966599984077485No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11370.19259467544324724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.19242528698639302No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT11350.1922558985295388No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11130.188529352478746No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11110.18819057556503754No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10870.18412525260053628No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10590.17938237580861816No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10470.1773497143263675No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10240.17345377981872045No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10200.1727762259913036No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC9870.16718640691511438No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9670.16379863777802997No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9620.16295169549375887No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA9530.16142719938207092No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9450.16007209172723716No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9300.15753126487442387No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA9250.15668432259015277No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA9150.15499043802161058No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG9090.15397410728048527No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9050.1532965534530684No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG8950.1516026688845262No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8930.15126389197081777No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8820.14940061894542134No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT8340.1412699730164188No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC8310.14076180764585616No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8210.13906792307731397No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7800.132122996346291No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7800.132122996346291No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7690.13025972332089458No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7630.12924339257976925No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7590.12856583875235236No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG7490.1268719541838102No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7480.12670256572695598No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7460.12636378881324753No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA7450.12619440035639332No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7320.12399235041728847No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7290.1234841850467258No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC7120.12060458128020407No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT7120.12060458128020407No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7080.11992702745278722No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC7060.11958825053907877No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7040.11924947362537033No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7040.11924947362537033No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7040.11924947362537033No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7030.1190800851685161No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA7030.1190800851685161No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA6970.11806375442739078No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6920.1172168121431197No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6900.11687803522941126No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG6890.11670864677255705No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6890.11670864677255705No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6860.11620048140199438No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6800.11518415066086905No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT6770.11467598529030641No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6750.11433720837659797No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6680.11315148917861843No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT6580.11145760461007624No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6520.11044127386895092No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC6490.10993310849838826No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA6230.10552900862017857No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6210.10519023170647013No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6190.10485145479276169No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6130.10383512405163639No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6080.10298818176736528No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA6040.1023106279399484No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5930.10044735491455199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA303.5973766E-430.83333212
CCCCTTT250.005494205329.637
TAGAACT250.005494205329.621
ACTAGTG250.005494205329.68
TTGCATC250.005494205329.619
GCCACAA250.005494205329.618
CGCTCTC1800.026.72222329
GCCGCTC1950.026.56410427
TAAGTTC358.864262E-426.42857230
AAGTTCC358.864262E-426.42857231
CTGCCAT358.864262E-426.42857213
AGTTCCG358.864262E-426.42857232
GCCGGCA2050.026.17073215
GCTCTCT1850.026.030
CCGCTCT1950.025.61538528
GCCGTCC809.767973E-1025.437527
GGCAGCT2050.025.26829318
TTTACTA451.322147E-424.6666683
GGTATCA11050.024.6108591
CCGGCAG2150.024.09302316
Produced by FastQC (version 0.11.5)