FastQCFastQC Report
Fri 10 Feb 2017
ERR1633234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633839
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23860.37643628744838986No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17910.28256386874269335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.26536707271089344No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC14650.23113124941822766No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC14260.22497826735180382No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13180.20793924009093792No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC12710.20052410785704258No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12160.19184682545567566No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA11070.17465002942387578No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10790.17023250383772537No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10520.16597274702250886No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT10440.16471059685503733No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10330.16297514037476393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9340.1473560320523035No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA9330.14719826328136956No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA9150.14435842540455857No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC8790.1386787496509366No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG8530.13457676160665405No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA7990.1260572479762211No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7210.11375128384337348No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG7050.11122698350843037No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG7020.11075367719562855No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT6920.10917598948628911No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6790.10712499546414783No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG6740.10633615160947811No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA6690.1055473077548084No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT6660.10507400144200657No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6550.10333854496173318No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC6480.10223416356519557No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC6390.10081424462679008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT303.597673E-430.83333236
TCTATCG250.00549450529.631
GGTATCA4600.025.3369581
AGGACCG903.8198777E-922.6111135
TTACCGG502.7002487E-422.231
GACCGTT603.722892E-521.5833327
GTATCAA12300.021.3577231
TCGCCTA450.003823942520.55555735
TAAGGCG450.003823942520.5555575
ACGGTAC450.003823942520.5555573
GTGTTAT555.139597E-420.1818181
CGCCGTT555.139597E-420.18181825
CCGTTTA555.139597E-420.18181827
TACCGGG555.139597E-420.18181832
GGACCGT656.895888E-519.9230776
TCGCCAT1650.019.06060813
AACGGCC1850.019.037
GTCCTAG609.230585E-418.51
TTGCGGT500.00703148418.535
CGGGAAT609.230585E-418.517
Produced by FastQC (version 0.11.5)