##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633839
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867330662833936	33.0	33.0	33.0	33.0	33.0
2	31.98427834197643	33.0	33.0	33.0	33.0	33.0
3	31.70405260641898	33.0	33.0	33.0	27.0	33.0
4	31.984272031225593	33.0	33.0	33.0	33.0	33.0
5	32.08212180064653	33.0	33.0	33.0	33.0	33.0
6	35.26962682952611	37.0	37.0	37.0	33.0	37.0
7	35.41287140740788	37.0	37.0	37.0	33.0	37.0
8	35.53305176866681	37.0	37.0	37.0	33.0	37.0
9	35.640564875307454	37.0	37.0	37.0	33.0	37.0
10	35.661691691423215	37.0	37.0	37.0	33.0	37.0
11	35.67513043533137	37.0	37.0	37.0	33.0	37.0
12	35.66311949880017	37.0	37.0	37.0	33.0	37.0
13	35.68022478894483	37.0	37.0	37.0	33.0	37.0
14	35.64134583072357	37.0	37.0	37.0	33.0	37.0
15	35.66993037664139	37.0	37.0	37.0	33.0	37.0
16	35.63556202758114	37.0	37.0	37.0	33.0	37.0
17	35.65042542349082	37.0	37.0	37.0	33.0	37.0
18	35.62244513196569	37.0	37.0	37.0	33.0	37.0
19	35.619351286367674	37.0	37.0	37.0	33.0	37.0
20	35.623416987594645	37.0	37.0	37.0	33.0	37.0
21	35.61754956700361	37.0	37.0	37.0	33.0	37.0
22	35.535003368363256	37.0	37.0	37.0	33.0	37.0
23	35.58470210889516	37.0	37.0	37.0	33.0	37.0
24	35.560478291805964	37.0	37.0	37.0	33.0	37.0
25	35.58456327237674	37.0	37.0	37.0	33.0	37.0
26	35.478787199904076	37.0	37.0	37.0	33.0	37.0
27	35.49533714397505	37.0	37.0	37.0	33.0	37.0
28	35.51775135326163	37.0	37.0	37.0	33.0	37.0
29	35.53798046507078	37.0	37.0	37.0	33.0	37.0
30	35.550409488844956	37.0	37.0	37.0	33.0	37.0
31	35.526958738733335	37.0	37.0	37.0	33.0	37.0
32	35.518410826724136	37.0	37.0	37.0	33.0	37.0
33	35.52303818477563	37.0	37.0	37.0	33.0	37.0
34	35.50065710693094	37.0	37.0	37.0	33.0	37.0
35	35.439819891171105	37.0	37.0	37.0	33.0	37.0
36	35.46064221355896	37.0	37.0	37.0	33.0	37.0
37	35.472949755379524	37.0	37.0	37.0	33.0	37.0
38	35.44863916546631	37.0	37.0	37.0	33.0	37.0
39	35.36452316755517	37.0	37.0	37.0	33.0	37.0
40	35.152104872057414	37.0	37.0	37.0	33.0	37.0
41	35.32549906206466	37.0	37.0	37.0	33.0	37.0
42	35.3773497686321	37.0	37.0	37.0	33.0	37.0
43	35.04295097019906	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	16.0
16	36.0
17	31.0
18	28.0
19	22.0
20	29.0
21	90.0
22	269.0
23	648.0
24	1307.0
25	2374.0
26	3865.0
27	6067.0
28	8606.0
29	11337.0
30	15070.0
31	19579.0
32	24785.0
33	32523.0
34	47827.0
35	96791.0
36	362538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56203546957508	21.53259739460652	14.028956880217217	23.876410255601186
2	15.514823164873098	22.32522769977865	39.74305777965698	22.41689135569127
3	17.243180050454455	27.681635241756975	31.794667100004894	23.28051760778368
4	12.442749657247345	18.227341643540395	39.79780354317106	29.532105156041204
5	12.899963555413915	38.54196412653687	35.960393727744744	12.59767859030448
6	28.259384480917078	40.372397406912484	18.07951230517529	13.288705806995152
7	24.794466733665804	33.42678503531654	24.29859948662042	17.480148744397237
8	23.42408718933357	36.89170278256781	21.146537212131157	18.53767281596746
9	25.014869706660527	16.19890855564268	20.413543502372054	38.37267823532474
10	14.238947114330294	28.912073886270807	34.69051289049743	22.15846610890147
11	32.04741267104107	24.52783751078744	25.157650444355745	18.26709937381575
12	21.498519340084783	26.81532692055869	30.508378310580447	21.177775428776076
13	26.29894973329189	23.06689869193912	27.70072526303998	22.933426311729004
14	21.222897297263184	22.152470895605983	28.01263412317639	28.611997683954442
15	23.47362658340683	29.472468560628172	25.755594086195387	21.29831076976961
16	22.542948603667494	29.846380547741617	25.05557404956148	22.555096799029407
17	21.997857500090717	28.543999343681914	26.534340739525337	22.92380241670203
18	21.018428970132792	28.74121030734934	28.57634194172337	21.664018780794493
19	23.945670746041188	27.4293629770336	28.660590465402098	19.964375811523116
20	23.6134097144543	26.45324759126529	29.294663155785617	20.63867953849479
21	21.33144221166574	28.614837521831255	27.7565754079506	22.297144858552407
22	21.228892510558676	28.53737305530269	27.597702255620117	22.63603217851852
23	21.35037446417781	28.446498243244733	28.36224971956601	21.840877573011444
24	23.039920232109417	28.66027492786023	26.78755961687432	21.512245223156036
25	22.647075992483895	27.45460598038303	28.15099733528546	21.747320691847612
26	22.403007703849084	27.7658837654357	27.81936737878231	22.011741151932902
27	22.09598967561163	27.52023778909155	27.617107814444992	22.766664720851825
28	21.016851282423453	28.19785466025284	28.529326847985054	22.25596720933865
29	22.89919048843634	28.464010576818406	27.180561625270773	21.45623730947449
30	21.69068170308233	28.551256707144873	27.84382153827707	21.914240051495725
31	22.29367394559186	27.945897933071333	28.090098589704958	21.67032953163185
32	20.960212293658167	28.003956840775025	28.291253772645735	22.744577092921073
33	21.36962225423175	27.655918932094743	28.652859795626334	22.32159901804717
34	21.42152817986902	27.903773671231967	28.289518316165463	22.38517983273355
35	22.24050586978712	28.61073553378697	27.340696927768725	21.80806166865718
36	22.639503091479067	28.359567650460132	27.11019675343423	21.89073250462657
37	22.84681125648627	26.90162643825956	27.3777725889382	22.873789716315972
38	22.826301316264853	27.971614242733565	27.55983775059597	21.64224669040561
39	22.139218318847533	27.784816017947776	27.985813432117617	22.090152231087075
40	22.148684445103566	27.748371431862033	28.027148850102314	22.075795272932087
41	21.6147949242631	27.87490198615106	28.294724685606283	22.21557840397956
42	22.177556130184477	27.78891800599206	27.81668530967643	22.21684055414703
43	22.010479001765432	27.21968828046239	27.883894806094293	22.885937911677885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	30.0
2	45.0
3	164.5
4	284.0
5	284.0
6	323.5
7	363.0
8	353.5
9	344.0
10	540.0
11	736.0
12	736.0
13	1089.5
14	1443.0
15	2925.0
16	4407.0
17	5018.0
18	5629.0
19	5629.0
20	5555.0
21	5481.0
22	6840.5
23	8200.0
24	9583.5
25	10967.0
26	10967.0
27	13346.0
28	15725.0
29	20223.5
30	24722.0
31	26424.0
32	28126.0
33	28126.0
34	32048.5
35	35971.0
36	37485.0
37	38999.0
38	44009.0
39	49019.0
40	49019.0
41	52085.5
42	55152.0
43	51477.5
44	47803.0
45	48089.5
46	48376.0
47	48376.0
48	47428.0
49	46480.0
50	47563.0
51	48646.0
52	48371.5
53	48097.0
54	48097.0
55	40564.0
56	33031.0
57	28180.5
58	23330.0
59	20061.5
60	16793.0
61	16793.0
62	14750.0
63	12707.0
64	10390.5
65	8074.0
66	6720.0
67	5366.0
68	5366.0
69	4405.5
70	3445.0
71	3013.5
72	2582.0
73	2078.0
74	1574.0
75	1574.0
76	1179.5
77	785.0
78	615.0
79	445.0
80	340.0
81	235.0
82	235.0
83	211.5
84	188.0
85	161.0
86	134.0
87	104.0
88	74.0
89	74.0
90	55.0
91	36.0
92	22.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	633839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.48602320430823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.99156977675702	39.1141453824046
2	7.517177097019593	6.838529837318551
3	2.0445059134222854	2.7898933025781436
4	1.0081384218016805	1.8342483058890369
5	0.573260273113013	1.3037665042463291
6	0.3826638443882314	1.0443513903175734
7	0.2922309992637782	0.9304698209471277
8	0.21628924511315661	0.7870510097647483
9	0.16140449446919122	0.6607483722634744
>10	1.367005776115549	12.853677875854405
>50	0.22376081086815247	7.181029795452966
>100	0.2043187180770598	17.950564602570022
>500	0.01307922589766646	3.838211462091983
>1k	0.004595403693774701	2.873312338301024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2386	0.37643628744838986	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1791	0.28256386874269335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1682	0.26536707271089344	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1465	0.23113124941822766	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1426	0.22497826735180382	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1318	0.20793924009093792	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1271	0.20052410785704258	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1216	0.19184682545567566	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1107	0.17465002942387578	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1079	0.17023250383772537	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1052	0.16597274702250886	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1044	0.16471059685503733	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1033	0.16297514037476393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.1473560320523035	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	933	0.14719826328136956	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	915	0.14435842540455857	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	879	0.1386787496509366	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	853	0.13457676160665405	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	799	0.1260572479762211	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	721	0.11375128384337348	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	705	0.11122698350843037	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	702	0.11075367719562855	No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT	692	0.10917598948628911	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	679	0.10712499546414783	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	674	0.10633615160947811	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	669	0.1055473077548084	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	666	0.10507400144200657	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	655	0.10333854496173318	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	648	0.10223416356519557	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	639	0.10081424462679008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.155375418678876E-4	0.0	0.0
2	0.0	0.0	3.155375418678876E-4	0.0	0.0
3	0.0	0.0	3.155375418678876E-4	0.0	0.0
4	0.0	0.0	3.155375418678876E-4	0.0	0.0
5	0.0	0.0	3.155375418678876E-4	0.0	0.0
6	0.0	0.0	4.7330631280183136E-4	0.0	0.0
7	0.0	0.0	0.0012621501674715504	0.0	0.0
8	0.0	0.0	0.0017354564802733816	0.0	0.0
9	0.0	0.0	0.002208762793075213	4.7330631280183136E-4	0.0
10	6.310750837357752E-4	0.0	0.002208762793075213	7.88843854669719E-4	0.0
11	6.310750837357752E-4	0.0	0.0029976066477449322	0.0011043813965376066	0.0
12	6.310750837357752E-4	0.0	0.0034709129605467633	0.0011043813965376066	0.0
13	6.310750837357752E-4	0.0	0.003786450502414651	0.0011043813965376066	0.0
14	6.310750837357752E-4	0.0	0.004259756815216483	0.0011043813965376066	0.0
15	6.310750837357752E-4	0.0	0.004417525586150426	0.0012621501674715504	0.0
16	6.310750837357752E-4	0.0	0.004733063128018314	0.0012621501674715504	0.0
17	6.310750837357752E-4	0.0	0.004733063128018314	0.0012621501674715504	0.0
18	6.310750837357752E-4	0.0	0.0050486006698862015	0.0014199189384054942	0.0
19	6.310750837357752E-4	0.0	0.005206369440820145	0.0014199189384054942	0.0
20	6.310750837357752E-4	0.0	0.005206369440820145	0.0014199189384054942	0.0
21	6.310750837357752E-4	0.0	0.005364138211754089	0.0018932252512073255	0.0
22	6.310750837357752E-4	0.0	0.0055219069826880325	0.0025243003349431008	0.0
23	6.310750837357752E-4	0.0	0.0055219069826880325	0.0026820691058770446	0.0
24	6.310750837357752E-4	0.0	0.005679675753621977	0.0034709129605467633	0.0
25	6.310750837357752E-4	0.0	0.005679675753621977	0.003786450502414651	0.0
26	6.310750837357752E-4	0.0	0.005679675753621977	0.0041019880442825386	0.0
27	6.310750837357752E-4	0.0	0.005679675753621977	0.0055219069826880325	0.0
28	6.310750837357752E-4	0.0	0.005679675753621977	0.014199189384054942	0.0
29	6.310750837357752E-4	0.0	0.005679675753621977	0.029818297706515378	0.0
30	6.310750837357752E-4	0.0	0.005679675753621977	0.05490353228501244	0.0
31	6.310750837357752E-4	0.0	0.005679675753621977	0.106651689151346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAT	30	3.597673E-4	30.833332	36
TCTATCG	25	0.005494505	29.6	31
GGTATCA	460	0.0	25.336958	1
AGGACCG	90	3.8198777E-9	22.611113	5
TTACCGG	50	2.7002487E-4	22.2	31
GACCGTT	60	3.722892E-5	21.583332	7
GTATCAA	1230	0.0	21.357723	1
TCGCCTA	45	0.0038239425	20.555557	35
TAAGGCG	45	0.0038239425	20.555557	5
ACGGTAC	45	0.0038239425	20.555557	3
GTGTTAT	55	5.139597E-4	20.181818	1
CGCCGTT	55	5.139597E-4	20.181818	25
CCGTTTA	55	5.139597E-4	20.181818	27
TACCGGG	55	5.139597E-4	20.181818	32
GGACCGT	65	6.895888E-5	19.923077	6
TCGCCAT	165	0.0	19.060608	13
AACGGCC	185	0.0	19.0	37
GTCCTAG	60	9.230585E-4	18.5	1
TTGCGGT	50	0.007031484	18.5	35
CGGGAAT	60	9.230585E-4	18.5	17
>>END_MODULE