FastQCFastQC Report
Fri 10 Feb 2017
ERR1633233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508004
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15780.31062747537420965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.22440768182927692No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7590.14940827237580806No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6830.13444776025385627No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6350.12499901575578146No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6230.12263682963126274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6100.12007779466303414No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5610.11043220132124945No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5400.10629837560334171No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5200.10236139872914388No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT5160.10157400335430429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG303.5966758E-430.83333418
ATTTGCG454.0024697E-628.77777716
GGTCGGG405.9337035E-527.7500021
GGTATCA2850.025.315791
GTATCAA8400.024.4464281
TGCGCGC400.001929711623.12500219
ACGGGTT450.003822913420.5555555
TTTGCGC450.003822913420.55555517
TAAGGCT450.003822913420.5555554
GTATTAT851.2425098E-619.5882361
TTAGATA701.21749865E-418.54
TTCGGTC609.227146E-418.528
TATAGGA701.21749865E-418.54
CGAGATT609.227146E-418.522
TATGACT500.007029611618.4999984
TATTAGA2050.018.048782
AACGGCC1750.017.9714337
GTCGATT852.7182743E-517.41176412
CGAACCG752.064407E-417.26666631
TGCGGTA650.001578293717.07692336
Produced by FastQC (version 0.11.5)