##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	508004
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841198100802355	33.0	33.0	33.0	27.0	33.0
2	31.95915780190707	33.0	33.0	33.0	33.0	33.0
3	31.681632428091117	33.0	33.0	33.0	27.0	33.0
4	31.965045550822435	33.0	33.0	33.0	33.0	33.0
5	32.06766679002528	33.0	33.0	33.0	33.0	33.0
6	35.23583672569507	37.0	37.0	37.0	33.0	37.0
7	35.37389272525413	37.0	37.0	37.0	33.0	37.0
8	35.51016527428918	37.0	37.0	37.0	33.0	37.0
9	35.62165061692428	37.0	37.0	37.0	33.0	37.0
10	35.62566633333596	37.0	37.0	37.0	33.0	37.0
11	35.63741427232856	37.0	37.0	37.0	33.0	37.0
12	35.62089668585287	37.0	37.0	37.0	33.0	37.0
13	35.62967023881702	37.0	37.0	37.0	33.0	37.0
14	35.609404650357085	37.0	37.0	37.0	33.0	37.0
15	35.62575097833875	37.0	37.0	37.0	33.0	37.0
16	35.60091652821632	37.0	37.0	37.0	33.0	37.0
17	35.612064078235605	37.0	37.0	37.0	33.0	37.0
18	35.58995401611011	37.0	37.0	37.0	33.0	37.0
19	35.5868123085645	37.0	37.0	37.0	33.0	37.0
20	35.58890481177313	37.0	37.0	37.0	33.0	37.0
21	35.58512334548547	37.0	37.0	37.0	33.0	37.0
22	35.480271808883394	37.0	37.0	37.0	33.0	37.0
23	35.54022212423524	37.0	37.0	37.0	33.0	37.0
24	35.515936882386754	37.0	37.0	37.0	33.0	37.0
25	35.5467141991008	37.0	37.0	37.0	33.0	37.0
26	35.442000456689314	37.0	37.0	37.0	33.0	37.0
27	35.44865985307202	37.0	37.0	37.0	33.0	37.0
28	35.467771907307814	37.0	37.0	37.0	33.0	37.0
29	35.46892544153196	37.0	37.0	37.0	33.0	37.0
30	35.4673782096204	37.0	37.0	37.0	33.0	37.0
31	35.46495696884276	37.0	37.0	37.0	33.0	37.0
32	35.46735852473603	37.0	37.0	37.0	33.0	37.0
33	35.473641939827246	37.0	37.0	37.0	33.0	37.0
34	35.46127195848852	37.0	37.0	37.0	33.0	37.0
35	35.40070944323273	37.0	37.0	37.0	33.0	37.0
36	35.41386878843473	37.0	37.0	37.0	33.0	37.0
37	35.41918764419178	37.0	37.0	37.0	33.0	37.0
38	35.41677821434477	37.0	37.0	37.0	33.0	37.0
39	35.30315706175542	37.0	37.0	37.0	33.0	37.0
40	35.11359556223967	37.0	37.0	37.0	33.0	37.0
41	35.29292682734781	37.0	37.0	37.0	33.0	37.0
42	35.32917063645168	37.0	37.0	37.0	33.0	37.0
43	35.011539279218276	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	11.0
16	23.0
17	22.0
18	14.0
19	13.0
20	32.0
21	80.0
22	216.0
23	561.0
24	1093.0
25	2045.0
26	3406.0
27	4975.0
28	7070.0
29	9531.0
30	12410.0
31	15928.0
32	20351.0
33	26599.0
34	38854.0
35	78624.0
36	286142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.213462886119004	21.503570838024896	13.55009015677042	24.732876119085674
2	16.01385028464343	21.540972118329776	38.44241383926111	24.00276375776569
3	17.375650585428463	27.178329304493666	30.26176959236541	25.18425051771246
4	12.339272919110874	18.02879504885788	39.87744190990622	29.75449012212502
5	13.354816103810208	37.557971984472566	36.04597601593688	13.041235895780348
6	29.35921764395556	40.403815717986475	17.404390516610103	12.832576121447865
7	26.234045401217315	32.268643553987765	24.151581483610364	17.34572956118456
8	24.186226880103305	36.08967645924048	20.82168644341383	18.902410217242384
9	25.739954803505483	15.474878150565743	20.580940307556634	38.204226738372135
10	14.71523846268927	28.1560775112007	34.57669624648625	22.551987779623783
11	33.58910559759372	23.661231013929022	24.622050220077007	18.12761316840025
12	22.531712348721662	26.272234076897032	30.30606058220014	20.889992992181163
13	27.28029700553539	22.38742214628231	26.35235155628696	23.97992929189534
14	21.487626081684397	21.18821899040165	27.764742009905437	29.55941291800852
15	23.617530570625426	29.227722616357347	24.406894433902096	22.747852379115123
16	22.90513460523933	28.71985259958583	25.326178534027292	23.04883426114755
17	22.158683789891416	27.96021291170936	26.688766230187166	23.19233706821206
18	22.468523869890788	28.02261399516539	27.949189376461604	21.55967275848222
19	24.28524184848938	27.018291194557527	27.35765860111338	21.338808355839717
20	23.460642042188645	26.8844339808348	27.760017637656397	21.894906339320166
21	22.655923969102606	27.369666380579684	27.840725663577455	22.133683986740262
22	22.426791915024292	27.40332753285407	27.399390555979874	22.770489996141762
23	22.442342973677373	27.269076621443926	27.960803458240484	22.327776946638213
24	22.667144353194068	28.02458248360249	26.157864898701582	23.150408264501856
25	22.787222147857104	27.400374800198424	27.599979527720254	22.212423524224217
26	22.749427169864806	27.579310399130712	27.615136888685914	22.056125542318565
27	22.708088912685728	27.5173030133621	26.788174896260657	22.986433177691517
28	22.17935291848096	27.126951756285383	28.149187801670855	22.544507523562807
29	23.61733372178172	27.050180707238525	27.04092881158416	22.291556759395593
30	23.207691278021432	26.937976866323886	27.392107148762605	22.462224706892073
31	22.95493736269793	27.179510397555923	27.353327926551756	22.512224313194384
32	22.022070692356756	26.932661947543718	27.816119558113716	23.22914780198581
33	21.874040361886916	27.182069432524152	28.367099471657703	22.57679073393123
34	22.83249738191038	26.749395674049808	27.886788293005566	22.531318651034244
35	22.596869315989636	27.62734151699593	27.363957764112097	22.411831402902337
36	23.875796253572805	27.060613695955148	26.768293163045957	22.295296887426083
37	22.83170998653554	26.63955401925969	27.340335902866908	23.18840009133786
38	22.864977441122512	27.459626302155097	26.965338855599562	22.71005740112283
39	22.418917961275895	27.141321721876206	27.554704293666983	22.88505602318092
40	22.513995952787774	27.857260966449083	27.65706569239612	21.97167738836702
41	22.46714592798482	26.72026204518075	27.736986322942336	23.075605703892098
42	22.18604577916709	27.525373815954207	27.66887662301872	22.619703781859986
43	23.10237714663664	26.429122605333816	27.486397744899648	22.982102503129898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	20.0
2	33.0
3	103.0
4	173.0
5	173.0
6	217.5
7	262.0
8	231.5
9	201.0
10	286.0
11	371.0
12	371.0
13	605.5
14	840.0
15	1563.5
16	2287.0
17	2735.0
18	3183.0
19	3183.0
20	3279.5
21	3376.0
22	3917.5
23	4459.0
24	5883.5
25	7308.0
26	7308.0
27	9503.5
28	11699.0
29	13746.5
30	15794.0
31	18090.0
32	20386.0
33	20386.0
34	24599.5
35	28813.0
36	31356.0
37	33899.0
38	36057.0
39	38215.0
40	38215.0
41	39731.5
42	41248.0
43	42075.5
44	42903.0
45	41838.5
46	40774.0
47	40774.0
48	38505.0
49	36236.0
50	35823.5
51	35411.0
52	33938.5
53	32466.0
54	32466.0
55	30778.5
56	29091.0
57	24809.0
58	20527.0
59	19469.5
60	18412.0
61	18412.0
62	16032.0
63	13652.0
64	11548.5
65	9445.0
66	7902.5
67	6360.0
68	6360.0
69	5280.5
70	4201.0
71	3415.0
72	2629.0
73	2043.0
74	1457.0
75	1457.0
76	1134.0
77	811.0
78	622.5
79	434.0
80	347.5
81	261.0
82	261.0
83	207.0
84	153.0
85	141.0
86	129.0
87	92.0
88	55.0
89	55.0
90	43.0
91	31.0
92	21.5
93	12.0
94	6.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	508004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.16490604254203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.64084053942915	51.26097189962192
2	7.367220740805464	8.717618458488444
3	2.104523703522889	3.73541841549703
4	0.9729523271032084	2.3025853206773577
5	0.5636453176032331	1.667401112865703
6	0.3806155364456558	1.3511449471283374
7	0.2769985555942574	1.1472015460957865
8	0.20152674760649242	0.9538648869757766
9	0.1647586620991607	0.8773137686512598
>10	1.1212172725465515	13.101196795507422
>50	0.12767623290056113	5.262786622013216
>100	0.07430891842233078	7.990802560942396
>500	0.00303991029909535	1.0885285237196327
>1k	6.755356220211888E-4	0.5431651418156713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1578	0.31062747537420965	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1140	0.22440768182927692	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	759	0.14940827237580806	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	683	0.13444776025385627	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	635	0.12499901575578146	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	623	0.12263682963126274	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.12007779466303414	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	561	0.11043220132124945	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	540	0.10629837560334171	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	520	0.10236139872914388	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	516	0.10157400335430429	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9684884370989205E-4	0.0
5	0.0	0.0	0.0	1.9684884370989205E-4	0.0
6	0.0	0.0	1.9684884370989205E-4	1.9684884370989205E-4	0.0
7	0.0	0.0	7.873953748395682E-4	1.9684884370989205E-4	0.0
8	0.0	0.0	9.842442185494602E-4	1.9684884370989205E-4	0.0
9	1.9684884370989205E-4	0.0	0.0013779419059692444	7.873953748395682E-4	0.0
10	5.905465311296761E-4	0.0	0.0013779419059692444	7.873953748395682E-4	0.0
11	5.905465311296761E-4	0.0	0.003740128030487949	7.873953748395682E-4	0.0
12	5.905465311296761E-4	0.0	0.003936976874197841	7.873953748395682E-4	0.0
13	5.905465311296761E-4	0.0	0.003936976874197841	7.873953748395682E-4	0.0
14	5.905465311296761E-4	0.0	0.004330674561617625	9.842442185494602E-4	0.0
15	5.905465311296761E-4	0.0	0.005511767623876978	9.842442185494602E-4	0.0
16	5.905465311296761E-4	0.0	0.006299162998716546	9.842442185494602E-4	0.0
17	5.905465311296761E-4	0.0	0.0068897095298462215	0.0011810930622593522	0.0
18	5.905465311296761E-4	0.0	0.0068897095298462215	0.0013779419059692444	0.0
19	5.905465311296761E-4	0.0	0.0068897095298462215	0.0015747907496791364	0.0
20	5.905465311296761E-4	0.0	0.0068897095298462215	0.0015747907496791364	0.0
21	5.905465311296761E-4	0.0	0.0068897095298462215	0.0019684884370989204	0.0
22	5.905465311296761E-4	0.0	0.0068897095298462215	0.0023621861245187044	0.0
23	5.905465311296761E-4	0.0	0.0068897095298462215	0.002755883811938489	0.0
24	5.905465311296761E-4	0.0	0.0068897095298462215	0.0033464303430681647	0.0
25	5.905465311296761E-4	0.0	0.007086558373556114	0.003543279186778057	0.0
26	5.905465311296761E-4	0.0	0.007086558373556114	0.004133825717907733	0.0
27	5.905465311296761E-4	0.0	0.007086558373556114	0.005708616467586869	0.0
28	5.905465311296761E-4	0.0	0.007086558373556114	0.013582570215982552	0.0
29	5.905465311296761E-4	0.0	0.007086558373556114	0.026377745057125535	0.0
30	5.905465311296761E-4	0.0	0.007086558373556114	0.04980275745860269	0.0
31	5.905465311296761E-4	0.0	0.007086558373556114	0.09881811954236581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGCG	30	3.5966758E-4	30.833334	18
ATTTGCG	45	4.0024697E-6	28.777777	16
GGTCGGG	40	5.9337035E-5	27.750002	1
GGTATCA	285	0.0	25.31579	1
GTATCAA	840	0.0	24.446428	1
TGCGCGC	40	0.0019297116	23.125002	19
ACGGGTT	45	0.0038229134	20.555555	5
TTTGCGC	45	0.0038229134	20.555555	17
TAAGGCT	45	0.0038229134	20.555555	4
GTATTAT	85	1.2425098E-6	19.588236	1
TTAGATA	70	1.21749865E-4	18.5	4
TTCGGTC	60	9.227146E-4	18.5	28
TATAGGA	70	1.21749865E-4	18.5	4
CGAGATT	60	9.227146E-4	18.5	22
TATGACT	50	0.0070296116	18.499998	4
TATTAGA	205	0.0	18.04878	2
AACGGCC	175	0.0	17.97143	37
GTCGATT	85	2.7182743E-5	17.411764	12
CGAACCG	75	2.064407E-4	17.266666	31
TGCGGTA	65	0.0015782937	17.076923	36
>>END_MODULE