FastQCFastQC Report
Fri 10 Feb 2017
ERR1633232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722659
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24130.33390575638025677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.23884017219739875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9380.1297984249832909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG250.005495009529.65
GGTATCA4050.026.0370371
GTATCAA11450.023.1048031
GCGTCAA450.003824454320.55555526
CCGGCAG1052.2559107E-819.38095316
CGGTTCG500.00703241618.522
GCCGGCA1103.8457074E-818.515
CCGCTCT1002.8712566E-718.528
TTAACGG1205.164111E-918.535
CGCCGCT1002.8712566E-718.526
TTCGCCG1002.8712566E-718.524
TCGCCGC1156.395203E-817.69565225
CGCTCTC1054.7911635E-717.6190529
GTCAACC2250.017.26666615
GCCGCTC1151.240558E-616.08695627
TAGGACC1851.8189894E-1116.04
TAACGGC1403.466448E-815.85714236
CCCACGA700.002591244915.85714219
CTGCTCG3050.015.7704939
ATTGACG1302.584875E-715.65384617
Produced by FastQC (version 0.11.5)