##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722659
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83713203599485	33.0	33.0	33.0	27.0	33.0
2	31.957826582108574	33.0	33.0	33.0	33.0	33.0
3	31.683967126957526	33.0	33.0	33.0	27.0	33.0
4	31.964257000881467	33.0	33.0	33.0	33.0	33.0
5	32.06915294765581	33.0	33.0	33.0	33.0	33.0
6	35.23352369513145	37.0	37.0	37.0	33.0	37.0
7	35.376802890436565	37.0	37.0	37.0	33.0	37.0
8	35.52427908598661	37.0	37.0	37.0	33.0	37.0
9	35.62721145104399	37.0	37.0	37.0	33.0	37.0
10	35.645366625199436	37.0	37.0	37.0	33.0	37.0
11	35.66381516040069	37.0	37.0	37.0	33.0	37.0
12	35.6439371819904	37.0	37.0	37.0	33.0	37.0
13	35.65155349895317	37.0	37.0	37.0	33.0	37.0
14	35.62826589027467	37.0	37.0	37.0	33.0	37.0
15	35.648969984460166	37.0	37.0	37.0	33.0	37.0
16	35.62031331513203	37.0	37.0	37.0	33.0	37.0
17	35.628858147480344	37.0	37.0	37.0	33.0	37.0
18	35.6062458227186	37.0	37.0	37.0	33.0	37.0
19	35.596624410683326	37.0	37.0	37.0	33.0	37.0
20	35.60158664044867	37.0	37.0	37.0	33.0	37.0
21	35.60400410151953	37.0	37.0	37.0	33.0	37.0
22	35.51995339433952	37.0	37.0	37.0	33.0	37.0
23	35.569640729583384	37.0	37.0	37.0	33.0	37.0
24	35.555858295544645	37.0	37.0	37.0	33.0	37.0
25	35.56648294700543	37.0	37.0	37.0	33.0	37.0
26	35.462269203040435	37.0	37.0	37.0	33.0	37.0
27	35.476023961508815	37.0	37.0	37.0	33.0	37.0
28	35.48992955183565	37.0	37.0	37.0	33.0	37.0
29	35.50455470699182	37.0	37.0	37.0	33.0	37.0
30	35.510571375987844	37.0	37.0	37.0	33.0	37.0
31	35.50275441113997	37.0	37.0	37.0	33.0	37.0
32	35.48311582641329	37.0	37.0	37.0	33.0	37.0
33	35.49489316537952	37.0	37.0	37.0	33.0	37.0
34	35.46478076105051	37.0	37.0	37.0	33.0	37.0
35	35.40807074982806	37.0	37.0	37.0	33.0	37.0
36	35.445229354370454	37.0	37.0	37.0	33.0	37.0
37	35.43984783971417	37.0	37.0	37.0	33.0	37.0
38	35.43890271898641	37.0	37.0	37.0	33.0	37.0
39	35.336022937512716	37.0	37.0	37.0	33.0	37.0
40	35.138408294921945	37.0	37.0	37.0	33.0	37.0
41	35.31495075824144	37.0	37.0	37.0	33.0	37.0
42	35.35234045379633	37.0	37.0	37.0	33.0	37.0
43	35.038686296026206	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	29.0
16	36.0
17	33.0
18	26.0
19	20.0
20	54.0
21	117.0
22	312.0
23	723.0
24	1586.0
25	2820.0
26	4607.0
27	6912.0
28	9850.0
29	13228.0
30	17289.0
31	22391.0
32	28647.0
33	38151.0
34	54837.0
35	111476.0
36	409511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70024728122116	21.046579368692562	13.636583782946039	24.61658956714024
2	15.86252990691322	21.70166011908798	38.74469147966053	23.69111849433827
3	17.635703699808623	27.270815142411564	30.205809378974042	24.88767177880577
4	12.513647515633238	17.98358561922013	39.78501616945198	29.71775069569465
5	13.575144016749254	37.23858694072861	35.96952366192077	13.216745380601363
6	29.345375896515506	40.12445703990402	17.676801921791604	12.853365141788867
7	26.174170666939734	32.20979742866275	24.114554720829602	17.501477183567907
8	24.373044547981827	36.08728321379793	20.829741274930498	18.70993096328974
9	25.585096151850315	15.610682216647131	20.617192894574067	38.18702873692848
10	14.636363762161682	28.318473858348124	34.43200735063149	22.6131550288587
11	33.66401027317172	23.766949557121684	24.68979145073956	17.879248718967037
12	22.421086570567862	25.838604376337944	30.97629725776611	20.764011795328084
13	27.69978648297468	22.3794348371777	26.491332703252844	23.42944597659477
14	21.623476632824058	21.14302873139337	27.98747403685556	29.246020598927018
15	23.421420061190688	29.23702603856037	24.44195671817552	22.899597182073425
16	22.767861467165012	28.663864976427334	25.432188625617336	23.136084930790318
17	22.089533237668114	28.082401243186624	26.861493456803277	22.96657206234199
18	22.70337738822875	27.885766315786558	27.833321109956426	21.577535186028264
19	24.200348988942224	26.898440343232423	27.523354721936627	21.377855945888726
20	23.589549151121066	26.912416506263675	27.659380150250673	21.838654192364586
21	22.816570470996695	27.226119096281927	27.81284118789083	22.14446924483055
22	22.48764631728104	27.224873695615777	27.588392312280064	22.69908767482312
23	22.58188163435313	27.107529277293995	27.967132492641756	22.343456595711118
24	22.702962254673366	27.630459179225607	26.770855963877842	22.895722602223177
25	22.9434629610923	27.129116222173945	27.645403987219417	22.282016829514333
26	22.761219330278873	27.43548478604708	27.5925436478339	22.21075223584014
27	22.7580366396876	27.41196055124201	27.119153016844738	22.710849792225655
28	22.475607444174916	27.092446091448387	27.930323984064408	22.501622480312292
29	23.470267442874164	27.17187497837846	27.05632947213001	22.301528106617365
30	23.152967028709252	26.914215418337	27.560301608365773	22.372515944587974
31	23.083224591404797	27.063801876126913	27.34512404882524	22.507849483643046
32	22.2930870576579	26.966383868463552	27.74199172777202	22.99853734610653
33	22.29613137039738	27.070444013013052	28.03272359439238	22.600701022197192
34	22.87150647815913	26.842950824662807	27.78516561753192	22.50037707964614
35	22.66988994809447	27.47478409595674	27.399229788876912	22.456096167071884
36	23.447296719476267	27.07860830626893	27.146828587203647	22.327266387051154
37	22.83110014543512	26.71813400234412	27.423307535089165	23.027458317131593
38	22.905132296145208	27.225012073467568	27.279948080630007	22.589907549757214
39	22.59336699605208	27.109189811515527	27.52100229845612	22.776440893976275
40	22.675425062166248	27.418187554572764	27.72663178622282	22.179755597038163
41	22.543412591554247	26.707063774200556	27.848542673653824	22.900980960591372
42	22.283954119439457	27.431610206196837	27.694666502458283	22.58976917190542
43	22.98386929381631	26.600512828318752	27.483086767064414	22.93253111080053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	33.5
2	50.0
3	155.5
4	261.0
5	261.0
6	324.0
7	387.0
8	350.0
9	313.0
10	455.5
11	598.0
12	598.0
13	948.0
14	1298.0
15	2355.5
16	3413.0
17	4179.0
18	4945.0
19	4945.0
20	5026.0
21	5107.0
22	6179.0
23	7251.0
24	9360.5
25	11470.0
26	11470.0
27	14582.5
28	17695.0
29	20890.0
30	24085.0
31	27369.0
32	30653.0
33	30653.0
34	35605.5
35	40558.0
36	43332.0
37	46106.0
38	49453.0
39	52800.0
40	52800.0
41	54545.5
42	56291.0
43	57133.0
44	57975.0
45	56860.0
46	55745.0
47	55745.0
48	53880.5
49	52016.0
50	51122.5
51	50229.0
52	47690.0
53	45151.0
54	45151.0
55	42745.5
56	40340.0
57	35862.5
58	31385.0
59	29070.5
60	26756.0
61	26756.0
62	23559.5
63	20363.0
64	17139.0
65	13915.0
66	11808.0
67	9701.0
68	9701.0
69	7942.5
70	6184.0
71	5104.5
72	4025.0
73	3242.0
74	2459.0
75	2459.0
76	1848.0
77	1237.0
78	993.0
79	749.0
80	620.0
81	491.0
82	491.0
83	395.0
84	299.0
85	236.5
86	174.0
87	143.5
88	113.0
89	113.0
90	77.0
91	41.0
92	24.0
93	7.0
94	6.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	722659.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.921136080413085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6433211887277	54.74418388083188
2	8.40841867534795	10.74951348736006
3	2.2721363571789484	4.357126118414689
4	1.030907985417545	2.635872384890376
5	0.6208793840812811	1.9843657799691314
6	0.38848749152916545	1.4899537086944468
7	0.24798497060750674	1.1096036736469774
8	0.18753151606237375	0.9589782046071332
9	0.15821853427957966	0.9102157614115662
>10	0.9117146689345613	11.098030443987433
>50	0.08423462050875005	3.751573273497652
>100	0.044195500851488864	5.017918577446574
>500	0.001531527256964245	0.6138139530145235
>1k	4.375792162754985E-4	0.57885075222764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	0.33390575638025677	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.23884017219739875	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.1297984249832909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.3837785179455318E-4	0.0	0.0
2	2.7675570358910636E-4	0.0	1.3837785179455318E-4	0.0	0.0
3	2.7675570358910636E-4	0.0	2.7675570358910636E-4	0.0	0.0
4	2.7675570358910636E-4	0.0	4.151335553836595E-4	0.0	0.0
5	2.7675570358910636E-4	0.0	4.151335553836595E-4	0.0	0.0
6	2.7675570358910636E-4	0.0	4.151335553836595E-4	0.0	0.0
7	2.7675570358910636E-4	0.0	0.0015221563697400849	1.3837785179455318E-4	0.0
8	2.7675570358910636E-4	0.0	0.0017989120733291912	1.3837785179455318E-4	0.0
9	2.7675570358910636E-4	0.0	0.0019372899251237443	6.918892589727659E-4	0.0
10	4.151335553836595E-4	0.0	0.002352423480507404	8.30267110767319E-4	0.0
11	4.151335553836595E-4	0.0	0.0029059348876856166	8.30267110767319E-4	0.0
12	4.151335553836595E-4	0.0	0.003182690591274723	8.30267110767319E-4	0.0
13	4.151335553836595E-4	0.0	0.004289713405631148	9.686449625618722E-4	0.0
14	4.151335553836595E-4	0.0	0.004704846961014808	9.686449625618722E-4	0.0
15	4.151335553836595E-4	0.0	0.005535114071782127	9.686449625618722E-4	0.0
16	4.151335553836595E-4	0.0	0.006365381182549446	9.686449625618722E-4	0.0
17	5.535114071782127E-4	0.0	0.006365381182549446	9.686449625618722E-4	0.0
18	5.535114071782127E-4	0.0	0.0065037590343439994	0.0012454006661509786	0.0
19	5.535114071782127E-4	0.0	0.006642136886138552	0.0015221563697400849	0.0
20	5.535114071782127E-4	0.0	0.006642136886138552	0.001660534221534638	0.0
21	5.535114071782127E-4	0.0	0.0069188925897276585	0.001660534221534638	0.0
22	5.535114071782127E-4	0.0	0.007057270441522212	0.0020756677769182977	0.0
23	5.535114071782127E-4	0.0	0.007195648293316765	0.002490801332301957	0.0
24	5.535114071782127E-4	0.0	0.007472403996905871	0.003182690591274723	0.0
25	5.535114071782127E-4	0.0	0.007610781848700425	0.003182690591274723	0.0
26	5.535114071782127E-4	0.0	0.007610781848700425	0.0035978241466583824	0.0
27	6.918892589727659E-4	0.0	0.007610781848700425	0.004289713405631148	0.0
28	6.918892589727659E-4	0.0	0.007610781848700425	0.01314589592048255	0.0
29	6.918892589727659E-4	0.0	0.007610781848700425	0.03016637169121259	0.0
30	6.918892589727659E-4	0.0	0.007610781848700425	0.05036953805321735	0.0
31	6.918892589727659E-4	0.0	0.007610781848700425	0.10267636603155846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGCG	25	0.0054950095	29.6	5
GGTATCA	405	0.0	26.037037	1
GTATCAA	1145	0.0	23.104803	1
GCGTCAA	45	0.0038244543	20.555555	26
CCGGCAG	105	2.2559107E-8	19.380953	16
CGGTTCG	50	0.007032416	18.5	22
GCCGGCA	110	3.8457074E-8	18.5	15
CCGCTCT	100	2.8712566E-7	18.5	28
TTAACGG	120	5.164111E-9	18.5	35
CGCCGCT	100	2.8712566E-7	18.5	26
TTCGCCG	100	2.8712566E-7	18.5	24
TCGCCGC	115	6.395203E-8	17.695652	25
CGCTCTC	105	4.7911635E-7	17.61905	29
GTCAACC	225	0.0	17.266666	15
GCCGCTC	115	1.240558E-6	16.086956	27
TAGGACC	185	1.8189894E-11	16.0	4
TAACGGC	140	3.466448E-8	15.857142	36
CCCACGA	70	0.0025912449	15.857142	19
CTGCTCG	305	0.0	15.770493	9
ATTGACG	130	2.584875E-7	15.653846	17
>>END_MODULE