FastQCFastQC Report
Fri 10 Feb 2017
ERR1633231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680486
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62060.9119952504533525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.6706971194117146No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC23260.34181452667652235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22860.33593637488500866No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG21840.3209470878166487No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19620.2883233453737476No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19330.28406168532490017No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA18030.2649576920024806No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT17660.2595204015953304No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17160.25217271185593826No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC16720.24570674488527317No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC16410.24115117724685003No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG15800.23218699576479165No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG15650.229982688842974No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14540.21367081762152346No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG14380.21131955690491797No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14120.20749875824043404No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13650.20059192988540545No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13640.2004449760906176No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13340.19603636224698232No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT12880.18927648768674155No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT12390.18207575174213725No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT12230.17972449102553176No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT12230.17972449102553176No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12190.17913667584638038No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT12180.17898972205159253No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC12170.1788427682568047No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG11890.1747280620027451No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC11770.172964616465291No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.1706133557486855No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11470.16855600262165568No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11430.16796818744250433No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11310.16620474190505022No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10880.15988572872917298No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10640.15635883765426475No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG10510.1544484383220228No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC10320.15165631622105377No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10310.15150936242626592No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG10280.1510685010419024No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG10230.1503337320679632No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10080.14812942514614555No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA9160.134609676025664No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9080.13343404566736125No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9010.13240536910384637No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT8950.13152364633511932No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8870.13034801597681658No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8860.13020106218202873No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8840.12990715459245303No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA8700.12784980146542324No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8680.12755589387584754No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC8570.12593940213318128No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG8540.12549854074881778No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8470.12446986418530287No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC8300.12197164967390953No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT8250.12123688069997031No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8170.12006125034166758No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC8130.1194734351625162No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC8100.11903257377815267No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA8080.11873866618857698No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT7830.11506482131888093No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA7760.11403614475536603No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG7760.11403614475536603No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA7670.11271356060227544No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7640.11227269921791191No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC7630.11212574542312406No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG7560.11109706885960915No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7390.10859885434821584No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG7390.10859885434821584No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT7380.108451900553428No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7350.10801103916906446No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7320.10757017778470093No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7270.10683540881076173No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7270.10683540881076173No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7250.10654150122118604No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA7240.1063945474263982No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7200.10580673224724682No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT7100.1043371942993684No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7070.10389633291500487No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC7050.10360242532542918No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6910.10154507219839938No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6840.10051639563488449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGG303.5979488E-430.8333329
AGCCATA250.005494787429.67
ACGGAGA250.005494787429.632
GACTTAC358.865662E-426.428571
ACTAGTG659.379983E-825.6153858
GGTATCA10050.024.8507461
CGAGTCG901.4188117E-1024.66666621
CTGGTAT451.3224373E-424.66666633
CTTTGTT451.3224373E-424.6666669
CGCCGTC901.4188117E-1024.66666626
GCCGTCC901.4188117E-1024.66666627
TCCCTTG1250.023.68000231
GTATCAA29400.023.1564621
CACGAAT400.00193038223.12512
TGCGGCG400.00193038223.1255
TTAAGCC400.00193038223.12531
CACAGAG400.00193038223.1252
CCCGGAC400.00193038223.12534
GCCGCTC1200.023.12499827
GCTCTCT1054.0017767E-1122.90476430
Produced by FastQC (version 0.11.5)