FastQCFastQC Report
Fri 10 Feb 2017
ERR1633230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122722
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32762.6694480207297797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30702.5015889571551964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18191.4822118283600334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.6225452649076775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7500.6111373673831912No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5630.4587604504489823No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.39520216424113036No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4280.34875572432000784No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4120.33571812714916643No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3990.3251250794478578No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3870.31534688156972673No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3620.29497563599028703No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3410.27786378970355763No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3130.25504799465458516No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG2970.24201039748374376No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2950.24038069783738858No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2620.21349065367252817No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2570.2094164045566402No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2540.20697185508710744No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2530.20615700526392988No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2490.20289760597121947No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2490.20289760597121947No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2420.19719365720897636No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2410.1963788073857988No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2390.19474910773944362No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2390.19474910773944362No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2300.18741545933084533No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2250.1833412102149574No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2180.17763726145271427No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2160.17600756180635907No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2160.17600756180635907No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2130.17356301233682633No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.16785906357458322No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2020.16459966428187284No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1930.15726601587327455No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1910.15563631622691937No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC1860.15156206711103143No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1860.15156206711103143No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1850.15074721728785384No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1840.14993236746467625No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1840.14993236746467625No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1458581183487883No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC1780.14504326852561072No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA1750.14259871905607796No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.14096901940972278No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1730.14096901940972278No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1730.14096901940972278No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA1700.13852446994019002No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1700.13852446994019002No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.13770962011701243No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1680.13689477029383484No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1680.13689477029383484No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1670.13607992047065726No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1670.13607992047065726No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC1610.13119082153159173No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1570.12793142223888138No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1570.12793142223888138No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1550.1263017225925262No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1530.12467202294617102No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1450.11815322436075032No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1430.11652352471439513No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1420.11570867489121754No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1400.11407897524486237No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1390.11326412542168479No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT1370.11163442577532962No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1350.11000472612897443No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1350.11000472612897443No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1340.10918987630579684No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA1340.10918987630579684No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG1330.10837502648261925No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1330.10837502648261925No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1300.1059304770130865No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1260.10267107772037613No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1260.10267107772037613No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1260.10267107772037613No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA1250.10185622789719855No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1250.10185622789719855No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1250.10185622789719855No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1240.10104137807402096No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1240.10104137807402096No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG1230.10022652825084338No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1230.10022652825084338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC251.2272096E-437.08
CAGCACT200.001835536737.04
AAACACG200.001835536737.015
CACTGTG200.001835536737.07
GACGTAT200.001835536737.028
CTGTGCA200.001835536737.09
TGGACGT200.001835536737.026
AGCACTG200.001835536737.05
GCACTGT200.001835536737.06
AAAGTGG200.001835536737.022
ACGAAAG200.001835536737.019
CAAACAC200.001835536737.014
ACACGAA250.005477465729.617
TATACGG250.005477465729.632
TGCAAAC250.005477465729.612
ACGTATA250.005477465729.629
CACGAAA250.005477465729.618
ACACAGC250.005477465729.61
GCACGTG250.005477465729.67
ATTGTAG250.005477465729.61
Produced by FastQC (version 0.11.5)