FastQCFastQC Report
Fri 10 Feb 2017
ERR1633229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553301
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20950.3786365829810537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.26513597481298606No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11670.21091593906390915No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9970.18019125213943224No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9390.1697087118946107No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9090.16428670831970302No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT8980.16229864034223687No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT8850.15994910545977686No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8510.15380416807488148No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8420.15217756700240917No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG8180.14783996414248302No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8070.14585189616501687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7860.1420564936625815No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7730.1397069587801215No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6610.11946481210046611No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6380.11530794269303689No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6270.11331987471557073No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6120.11060887292811687No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6070.10970520566563227No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5790.10464466899571842No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5720.10337953482823996No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5680.10265660101825227No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC5560.1004877995882892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC250.005493910529.612
CGGGTCT358.863556E-426.42857217
GGACCGT358.863556E-426.4285726
GGTATCA5050.025.6435621
CGTTTTA400.001929928723.12528
CGGAACT450.003823338320.5555554
GTATTGA701.2177471E-418.51
GTATCAA10200.018.51
AACTCGC500.007030383718.522
TATCGCC609.228566E-418.533
GGCCGTT1552.0008883E-1117.90322714
TTAACGG2400.017.72916635
GTATTAG3400.017.4117641
TTTAACG2450.017.36734834
TAACGGC2450.017.36734836
CCGTTAA1603.45608E-1117.3437516
TTATGCG752.0648257E-417.2666664
CTCTAAT2800.017.1785721
TGCGGTA1301.3884346E-817.07692336
CGTTAAA1655.4569682E-1116.8181817
Produced by FastQC (version 0.11.5)