FastQCFastQC Report
Fri 10 Feb 2017
ERR1633228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474668
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42000.8848289751995078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.6583548922615385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16620.3501394659003767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.17801916286752004No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA5540.11671315530012555No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5210.10976092763784372No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG5180.10912890694127263No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4750.10006994362375386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC250.005493133329.63
CGCCTGG659.3676135E-825.61538536
CCGTCCG851.9699655E-923.94117728
CGTCCGC851.9699655E-923.94117729
GGTATCA7200.022.8680551
TCGCCGT903.814421E-922.6111125
CGCCGTC903.814421E-922.6111126
GCCGTCC903.814421E-922.6111127
GTATCAA17250.021.4492741
GGACTGC705.091515E-621.1428596
GACCGTT450.003822549220.5555557
GTGTAGA450.003822549220.5555551
TCCGCCG1001.2849341E-820.3531
CGAGTCG1001.2849341E-820.3521
GAGTCGC1001.2849341E-820.3522
GCCGGCA1101.7462298E-920.18181815
CGGGATA555.136984E-420.18181817
GGACCGT555.136984E-420.1818186
TTAACGG759.246991E-619.73333435
GTCCGCC1052.2509994E-819.3809530