##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474668
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87057269502052	33.0	33.0	33.0	33.0	33.0
2	31.983971955134958	33.0	33.0	33.0	33.0	33.0
3	31.70883438529667	33.0	33.0	33.0	27.0	33.0
4	31.991284434594284	33.0	33.0	33.0	33.0	33.0
5	32.09083612124685	33.0	33.0	33.0	33.0	33.0
6	35.24421911736203	37.0	37.0	37.0	33.0	37.0
7	35.37256566695037	37.0	37.0	37.0	33.0	37.0
8	35.52596762368645	37.0	37.0	37.0	33.0	37.0
9	35.62936199617417	37.0	37.0	37.0	33.0	37.0
10	35.63880227864529	37.0	37.0	37.0	33.0	37.0
11	35.64484860997582	37.0	37.0	37.0	33.0	37.0
12	35.627088828402165	37.0	37.0	37.0	33.0	37.0
13	35.63965972005697	37.0	37.0	37.0	33.0	37.0
14	35.62552563054598	37.0	37.0	37.0	33.0	37.0
15	35.647640034719004	37.0	37.0	37.0	33.0	37.0
16	35.626246134140075	37.0	37.0	37.0	33.0	37.0
17	35.62646102117691	37.0	37.0	37.0	33.0	37.0
18	35.61398493262659	37.0	37.0	37.0	33.0	37.0
19	35.601378226465656	37.0	37.0	37.0	33.0	37.0
20	35.60604885941331	37.0	37.0	37.0	33.0	37.0
21	35.585712540133315	37.0	37.0	37.0	33.0	37.0
22	35.496698745228244	37.0	37.0	37.0	33.0	37.0
23	35.54468386324758	37.0	37.0	37.0	33.0	37.0
24	35.53680256516133	37.0	37.0	37.0	33.0	37.0
25	35.55517119333935	37.0	37.0	37.0	33.0	37.0
26	35.440410981991626	37.0	37.0	37.0	33.0	37.0
27	35.457201665163865	37.0	37.0	37.0	33.0	37.0
28	35.47416720739548	37.0	37.0	37.0	33.0	37.0
29	35.47747478237421	37.0	37.0	37.0	33.0	37.0
30	35.48025145996781	37.0	37.0	37.0	33.0	37.0
31	35.44758652363336	37.0	37.0	37.0	33.0	37.0
32	35.43158586633184	37.0	37.0	37.0	33.0	37.0
33	35.42614206139871	37.0	37.0	37.0	33.0	37.0
34	35.39772851761652	37.0	37.0	37.0	33.0	37.0
35	35.31861638029107	37.0	37.0	37.0	33.0	37.0
36	35.34686559869214	37.0	37.0	37.0	33.0	37.0
37	35.32120134494004	37.0	37.0	37.0	33.0	37.0
38	35.30863888022787	37.0	37.0	37.0	33.0	37.0
39	35.192715750798456	37.0	37.0	37.0	33.0	37.0
40	34.95688776155123	37.0	37.0	37.0	27.0	37.0
41	35.107218097701974	37.0	37.0	37.0	27.0	37.0
42	35.12420260055449	37.0	37.0	37.0	33.0	37.0
43	34.777952168673686	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	21.0
16	31.0
17	21.0
18	14.0
19	28.0
20	32.0
21	85.0
22	194.0
23	536.0
24	1099.0
25	1989.0
26	3153.0
27	4827.0
28	6702.0
29	9066.0
30	11964.0
31	15231.0
32	19200.0
33	25049.0
34	36527.0
35	73015.0
36	265881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.904868244752116	21.15899955337204	13.774680408201101	23.161451793674736
2	15.686543015328608	22.72620020730279	39.39363934370971	22.193617433658893
3	17.506130600756737	28.457995904505886	30.482990216319617	23.55288327841776
4	11.597158434948216	18.27466776778717	41.12958952362493	28.998584273639683
5	13.342378251746483	37.49989466321723	35.4873300917694	13.670396993266873
6	27.790371375361307	41.4005157288884	17.616102201960107	13.193010693790185
7	25.763691675023388	32.973783781506235	23.920719323822123	17.34180521964826
8	24.391364069202055	35.32258336352988	21.18680846402117	19.0992441032469
9	26.03672461594209	14.917795174732657	21.181330951317552	37.8641492580077
10	14.787177564107967	28.38131072665526	34.34358330454128	22.48792840469549
11	32.80882637970118	24.770787160710224	24.34438386409027	18.076002595498327
12	22.422830272948673	26.626821272973956	30.908761492243	20.041586961834376
13	27.62309656433549	23.288698627251048	26.765023131957495	22.323181676455963
14	21.56349279917753	21.979151743955775	29.32828840368426	27.12906705318244
15	23.26152173729849	29.326603015160067	26.3489428400482	21.062932407493236
16	21.977887702562633	29.2050443678529	26.25161165277626	22.56545627680821
17	21.670514970463568	27.762351791146656	27.186580936570405	23.380552301819378
18	22.096075572821423	26.992761256288606	29.595422484768303	21.315740686121668
19	24.151406878070567	26.610599408428627	29.236013381984883	20.001980331515924
20	23.501478928429975	26.54507992955076	29.298583430945417	20.654857711073845
21	21.898674441925724	27.514810351656315	29.288681773365806	21.297833433052155
22	22.07248013348277	26.998238768992223	29.317333378277027	21.61194771924798
23	21.45731332215359	27.006455038047644	30.007499978932646	21.52873166086612
24	21.734981081513816	28.00251965584366	28.50265870039691	21.75984056224561
25	21.70443341451288	27.793742152409685	29.624916783941618	20.876907649135816
26	22.319178878711014	27.375133777714105	29.016280853143673	21.289406490431205
27	22.117774950070366	27.267058238600455	28.77147817000514	21.84368864132404
28	21.478802025837005	27.10483959314721	29.73552040584156	21.680837975174228
29	22.780975334338947	27.469094187937674	28.7862253195918	20.963705158131578
30	22.484136280516065	26.970008511212047	29.163331001879207	21.382524206392677
31	21.86307060935222	26.890795250575138	29.37210850531319	21.87402563475945
32	20.86216049954916	27.207648293122773	29.53032435302148	22.399866854306588
33	21.164266392510132	26.862143645663917	30.034887542450722	21.93870241937523
34	21.798815171867496	27.09725534478836	29.67358237757759	21.430347105766558
35	21.62669486883464	27.812281426175765	28.767475372260193	21.7935483327294
36	22.089334018724667	27.401889320535616	28.73903444091449	21.769742219825226
37	21.91679236856076	27.129699073879003	29.127727169305707	21.825781388254526
38	21.800711233957205	27.090092443560554	28.68699807023014	22.4221982522521
39	21.46868969469187	27.107789023064544	29.233695972764124	22.18982530947947
40	21.777115794618553	27.85294142432184	29.10771318058095	21.262229600478648
41	21.777747815315127	27.02330892328954	28.955396192707322	22.24354706868801
42	20.90050308847447	28.42618419611181	28.755256305459813	21.918056409953905
43	22.10092106482847	26.82548644526279	28.668880143595104	22.404712346313634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	52.0
2	73.0
3	304.0
4	535.0
5	535.0
6	624.0
7	713.0
8	694.5
9	676.0
10	893.5
11	1111.0
12	1111.0
13	1810.5
14	2510.0
15	4221.5
16	5933.0
17	6696.5
18	7460.0
19	7460.0
20	6738.0
21	6016.0
22	6345.0
23	6674.0
24	8015.5
25	9357.0
26	9357.0
27	10989.5
28	12622.0
29	14257.0
30	15892.0
31	17985.0
32	20078.0
33	20078.0
34	23839.0
35	27600.0
36	29211.0
37	30822.0
38	31721.5
39	32621.0
40	32621.0
41	32791.5
42	32962.0
43	33801.0
44	34640.0
45	34251.0
46	33862.0
47	33862.0
48	32919.5
49	31977.0
50	32389.0
51	32801.0
52	32089.0
53	31377.0
54	31377.0
55	29824.5
56	28272.0
57	23912.5
58	19553.0
59	17706.0
60	15859.0
61	15859.0
62	13869.5
63	11880.0
64	9316.5
65	6753.0
66	5785.5
67	4818.0
68	4818.0
69	3995.5
70	3173.0
71	2955.5
72	2738.0
73	2355.0
74	1972.0
75	1972.0
76	1253.0
77	534.0
78	414.0
79	294.0
80	224.0
81	154.0
82	154.0
83	141.0
84	128.0
85	115.5
86	103.0
87	82.5
88	62.0
89	62.0
90	42.0
91	22.0
92	15.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	474668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.40094711515851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81320253021309	54.248024962704214
2	5.605082444312499	6.771045765900453
3	1.361205427642111	2.466542911436336
4	0.7167438098208339	1.7316801980842167
5	0.41332666386356504	1.2482660982654046
6	0.28621906218758136	1.037274146312542
7	0.23911218385981808	1.0109821660334852
8	0.1786544732092058	0.8632719510556595
9	0.14920445773472762	0.8110881504883068
>10	1.0092870979780162	12.282932127277844
>50	0.14513099096101367	6.115281520257087
>100	0.0803530120315953	8.970875531005303
>500	0.0014159121062836176	0.5212592245719752
>1k	0.001061934079712713	1.9214752466071943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4200	0.8848289751995078	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.6583548922615385	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1662	0.3501394659003767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.17801916286752004	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	554	0.11671315530012555	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	521	0.10976092763784372	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	518	0.10912890694127263	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	475	0.10006994362375386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.1067356552369236E-4	0.0	0.0
2	0.0	0.0	2.1067356552369236E-4	0.0	0.0
3	0.0	0.0	4.213471310473847E-4	0.0	0.0
4	0.0	0.0	4.213471310473847E-4	2.1067356552369236E-4	0.0
5	0.0	0.0	8.426942620947695E-4	4.213471310473847E-4	0.0
6	0.0	0.0	0.002317409220760616	4.213471310473847E-4	0.0
7	0.0	0.0	0.004845492007044924	4.213471310473847E-4	0.0
8	0.0	0.0	0.006109533400187078	4.213471310473847E-4	0.0
9	0.0	0.0	0.0069522276622818475	0.0027387563518080004	0.0
10	4.213471310473847E-4	0.0	0.007373574793329232	0.0029494299173316928	0.0
11	4.213471310473847E-4	0.0	0.011587046103803079	0.0029494299173316928	0.0
12	4.213471310473847E-4	0.0	0.012851087496945234	0.0029494299173316928	0.0
13	4.213471310473847E-4	0.0	0.013061761062468926	0.003370777048379078	0.0
14	4.213471310473847E-4	0.0	0.013904455324563695	0.00358145061390277	0.0
15	4.213471310473847E-4	0.0	0.015800517414276925	0.00358145061390277	0.0
16	4.213471310473847E-4	0.0	0.017696579503990156	0.00358145061390277	0.0
17	4.213471310473847E-4	0.0	0.017696579503990156	0.00358145061390277	0.0
18	6.32020696571077E-4	0.0	0.018539273766084927	0.0037921241794264624	0.0
19	6.32020696571077E-4	0.0	0.018539273766084927	0.004002797744950154	0.0
20	6.32020696571077E-4	0.0	0.01896062089713231	0.004424144875997539	0.0
21	6.32020696571077E-4	0.0	0.01980331515922708	0.005056165572568616	0.0
22	6.32020696571077E-4	0.0	0.020224662290274465	0.005266839138092309	0.0
23	6.32020696571077E-4	0.0	0.02064600942132185	0.00632020696571077	0.0
24	6.32020696571077E-4	0.0	0.020856682986845544	0.008216269055424002	0.0
25	6.32020696571077E-4	0.0	0.020856682986845544	0.008848289751995078	0.0
26	6.32020696571077E-4	0.0	0.020856682986845544	0.00990165757961354	0.0
27	6.32020696571077E-4	0.0	0.020856682986845544	0.012219066800374156	0.0
28	6.32020696571077E-4	0.0	0.020856682986845544	0.027387563518080006	0.0
29	6.32020696571077E-4	0.0	0.020856682986845544	0.05898859834663386	0.0
30	6.32020696571077E-4	0.0	0.020856682986845544	0.09796320796851694	0.0
31	6.32020696571077E-4	0.0	0.020856682986845544	0.17991522495723328	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	25	0.0054931333	29.6	3
CGCCTGG	65	9.3676135E-8	25.615385	36
CCGTCCG	85	1.9699655E-9	23.941177	28
CGTCCGC	85	1.9699655E-9	23.941177	29
GGTATCA	720	0.0	22.868055	1
TCGCCGT	90	3.814421E-9	22.61111	25
CGCCGTC	90	3.814421E-9	22.61111	26
GCCGTCC	90	3.814421E-9	22.61111	27
GTATCAA	1725	0.0	21.449274	1
GGACTGC	70	5.091515E-6	21.142859	6
GACCGTT	45	0.0038225492	20.555555	7
GTGTAGA	45	0.0038225492	20.555555	1
TCCGCCG	100	1.2849341E-8	20.35	31
CGAGTCG	100	1.2849341E-8	20.35	21
GAGTCGC	100	1.2849341E-8	20.35	22
GCCGGCA	110	1.7462298E-9	20.181818	15
CGGGATA	55	5.136984E-4	20.181818	17
GGACCGT	55	5.136984E-4	20.181818	6
TTAACGG	75	9.246991E-6	19.733334	35
GTCCGCC	105	2.2509994E-8	19.38095	30
>>END_MODULE