FastQCFastQC Report
Fri 10 Feb 2017
ERR1633227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253264
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71662.829458588666372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56972.249431423336913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31281.2350748625939731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.5614694547981552No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13400.5290921725946048No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10780.42564280750521194No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10600.4185355992166277No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9480.3743129698654369No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8950.35338618990460546No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.339171773327437No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7780.3071893360288079No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7730.30521511150420116No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6850.2704687598711226No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6770.26731000063175187No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6090.24046054709710024No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5870.23177395918883062No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5750.22703582032977446No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5570.2199286120411902No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5540.21874407732642617No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5470.21598016299197675No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5410.21361109356244867No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5350.2112420241329206No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5190.20492450565417902No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5160.20373997093941498No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5040.19900183208035882No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4990.1970276075557521No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4900.19347400341145998No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4720.18636679512287574No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4640.18320803588350495No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4590.18123381135889824No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4500.17768020721460612No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4500.17768020721460612No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4480.17689051740476341No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.17452144797523533No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4330.1709678438309432No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4270.16859877440141513No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4160.1642554804472803No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4150.16386063554235897No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4110.1622812559226736No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4090.1614915661128309No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4090.1614915661128309No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4020.15872765177838147No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3970.15675342725377472No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3890.15359466801440394No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3810.15043590877503318No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.14925137406026914No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3720.14688230463074106No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3710.1464874597258197No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3690.145697769915977No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3660.14451323520121295No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3580.1413544759618422No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.14095963105692083No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3560.1405647861519995No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3390.1338524227683366No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3370.1330627329584939No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3350.1322730431486512No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3320.13108850843388717No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3310.13069366352896583No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3270.12911428390928043No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3260.1287194390043591No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3240.12792974919451638No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3220.1271400593846737No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3210.12674521447975234No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3210.12674521447975234No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3200.126350369574831No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3190.12595552466990967No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3150.12437614505022426No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3140.12398130014530293No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3120.12319161033546022No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3050.1204276960010108No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3040.12003285109608945No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3040.12003285109608945No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3020.11924316128624676No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2990.11805862657148272No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2970.11726893676164002No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2910.11489986733211194No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2850.11253079790258386No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2820.11134626318781982No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2800.11055657337797713No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2770.10937203866321309No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2750.1085823488533704No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2720.10739781413860636No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2710.107002969233685No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.10621327942384232No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2680.10581843451892096No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2670.10542358961399961No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2660.10502874470907828No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2610.10305452018447153No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA2600.1026596752795502No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2580.10186998546970749No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2570.10147514056478615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC200.001839227637.02
CAGCACC200.001839227637.04
ACTAGTG801.8189894E-1230.0625028
ACAAACA250.005488355629.5999984
CTAGTGC453.992054E-628.7777779
CTAGTGG405.9221355E-527.7500029
GCCGGCA2650.025.13207615
GGCAGCT2600.024.90384718
CCGGCAG2700.024.66666816
TAGTGCA451.3192363E-424.66666610
TTCGCCG2350.024.40425724
GCAGCTT2600.024.19230819
CAGCTTC2650.023.7358520
CCGCTCT2350.023.61702228
CGCTCTC2350.023.61702229
CGCCGGC2750.023.54545414
GTGCAGG802.7046553E-823.12500215
CAGGTCG802.7046553E-823.12500218
CGTGCAG802.7046553E-823.12500214
TTATGCG400.001927054423.1250022
Produced by FastQC (version 0.11.5)