FastQCFastQC Report
Fri 10 Feb 2017
ERR1633226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522708
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13580.25980088309342886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.20604237930163688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5770.1103866786044981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2600.027.0384621
ATACTGC909.45347E-820.5555556
GTATCAA6100.019.4098361
CCTAATA609.2276325E-418.52
CTAACCG852.7185188E-517.4117646
AAAGCGT752.0645499E-417.26666635
GGCGTCA650.001578376417.07692325
GGCCGTT650.001578376417.07692314
CCGTTAA650.001578376417.07692316
ACCGACC904.4385E-516.4444458
CCGTGCA904.4385E-516.44444510
TAACCGT904.4385E-516.4444457
TATACTG1251.6525155E-716.2799995
TCTATAC1151.2389519E-616.0869563
GCCGTTA700.002589959415.85714215
AACCGTG957.0479065E-515.5789488
ATAAGAC957.0479065E-515.5789483
ATAACAC855.357205E-415.2352943
TCCGGTC855.357205E-415.23529423
TAAAGAC1001.091553E-414.83
Produced by FastQC (version 0.11.5)