##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633226.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522708
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51439044361288	33.0	33.0	33.0	27.0	33.0
2	31.71029905798266	33.0	33.0	33.0	27.0	33.0
3	31.435200915233743	33.0	33.0	33.0	27.0	33.0
4	31.754338942583622	33.0	33.0	33.0	27.0	33.0
5	31.860440245796887	33.0	33.0	33.0	33.0	33.0
6	34.87264400009183	37.0	37.0	37.0	27.0	37.0
7	35.01948124000398	37.0	37.0	37.0	33.0	37.0
8	35.156420028007986	37.0	37.0	37.0	33.0	37.0
9	35.27342416798672	37.0	37.0	37.0	33.0	37.0
10	35.314906219151034	37.0	37.0	37.0	33.0	37.0
11	35.2982162124934	37.0	37.0	37.0	33.0	37.0
12	35.31936186168951	37.0	37.0	37.0	33.0	37.0
13	35.31176297282613	37.0	37.0	37.0	33.0	37.0
14	35.29352717004522	37.0	37.0	37.0	33.0	37.0
15	35.29874423196125	37.0	37.0	37.0	33.0	37.0
16	35.285997153286345	37.0	37.0	37.0	33.0	37.0
17	35.2850712061036	37.0	37.0	37.0	33.0	37.0
18	35.2527166218998	37.0	37.0	37.0	33.0	37.0
19	35.262670554114344	37.0	37.0	37.0	33.0	37.0
20	35.25973392410294	37.0	37.0	37.0	33.0	37.0
21	35.25369422316092	37.0	37.0	37.0	33.0	37.0
22	35.151289056222595	37.0	37.0	37.0	33.0	37.0
23	35.20147577615035	37.0	37.0	37.0	33.0	37.0
24	35.18485846782525	37.0	37.0	37.0	33.0	37.0
25	35.20372177200272	37.0	37.0	37.0	33.0	37.0
26	35.07854672207045	37.0	37.0	37.0	33.0	37.0
27	35.098963474827244	37.0	37.0	37.0	33.0	37.0
28	35.101622703306624	37.0	37.0	37.0	33.0	37.0
29	35.10601521308264	37.0	37.0	37.0	33.0	37.0
30	35.127595139159915	37.0	37.0	37.0	33.0	37.0
31	35.11739441523757	37.0	37.0	37.0	33.0	37.0
32	35.09073325833926	37.0	37.0	37.0	33.0	37.0
33	35.09498037145022	37.0	37.0	37.0	33.0	37.0
34	35.05914391974104	37.0	37.0	37.0	33.0	37.0
35	35.01435600756063	37.0	37.0	37.0	27.0	37.0
36	35.049853838089334	37.0	37.0	37.0	27.0	37.0
37	35.04651162790697	37.0	37.0	37.0	27.0	37.0
38	35.05269289928603	37.0	37.0	37.0	27.0	37.0
39	34.93103032668335	37.0	37.0	37.0	27.0	37.0
40	34.743495412352594	37.0	37.0	37.0	27.0	37.0
41	34.92063829135961	37.0	37.0	37.0	27.0	37.0
42	34.978571209929825	37.0	37.0	37.0	27.0	37.0
43	34.593111259058595	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	19.0
16	25.0
17	27.0
18	22.0
19	28.0
20	70.0
21	151.0
22	487.0
23	911.0
24	1811.0
25	3130.0
26	4717.0
27	6775.0
28	9589.0
29	12456.0
30	15639.0
31	19267.0
32	24282.0
33	32001.0
34	46016.0
35	89981.0
36	255299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.93740290946379	21.429555315778597	13.862041522226558	24.77100025253105
2	15.67529098464152	22.077144409498228	38.56474360445985	23.6828210014004
3	17.660720708311334	27.42353283286271	30.172295048095688	24.743451410730273
4	12.848856340442467	18.3303106131913	39.475003252293824	29.345829794072408
5	13.959610336937638	37.368282100140036	35.54317133083863	13.128936232083689
6	29.497731046779464	39.945820611125136	17.80688261897656	12.749565723118836
7	26.111136619297966	32.22315327104234	24.20471850440399	17.460991605255707
8	24.234563083021495	36.37480199269956	21.11829166571011	18.27234325856884
9	25.394101486872213	15.652524927875602	20.65455282873038	38.298820756521806
10	14.424879665128524	28.558583377334955	34.602110547380185	22.41442641015634
11	33.42229313498167	23.855383885458036	24.791087949677447	17.931235029882842
12	22.09417112422232	25.580821414633025	31.54686746711357	20.778139994031083
13	27.54501557274807	22.2986830123128	26.61906838999977	23.537233024939354
14	21.40219778537922	21.209355892773786	28.025398501649107	29.36304782019789
15	23.208177414541197	29.38523994275963	24.192857197517544	23.21372544518163
16	22.698141218424052	28.694988406529077	25.62788401937602	22.978986355670852
17	22.09436243562371	28.280225288306283	26.938749741729605	22.686662534340396
18	22.70789809989516	27.931847226367303	27.587678015258998	21.77257665847854
19	23.692960505674296	27.264361746902672	27.50235313023715	21.540324617185885
20	23.3818881670072	27.220360124581983	27.562424910274952	21.835326798135863
21	22.687045157143185	27.51574492833475	27.707438952531817	22.08977096199025
22	22.55523160158253	27.23968257612281	27.66324601880974	22.54183980348493
23	22.529595873795692	27.24274355854511	27.77210220620308	22.45555836145611
24	22.47794179541924	27.597626208131498	27.250204703199493	22.674227293249768
25	22.89882687848665	27.197402756414675	27.636271111213144	22.267499253885532
26	22.839137721251635	27.463134292951324	27.33820794784086	22.35952003795618
27	22.65892238113823	27.66841142664738	27.171193094423657	22.501473097790736
28	22.409834936522877	27.438072499368673	27.788554986722993	22.36353757738546
29	23.17871545872648	27.220742747384776	27.27603174238772	22.32451005150103
30	22.829380839780526	27.25766584785387	27.45318610007882	22.45976721228678
31	22.715933178753723	27.16105359014976	27.575051462766975	22.547961768329543
32	22.30136137193232	27.362313184416536	27.582895230224143	22.753430213427002
33	22.262716468850677	27.274501251176563	27.9352908315924	22.527491448380356
34	22.75706513005349	26.93530613650451	27.858957582436084	22.448671151005918
35	22.63806943838625	27.456821016705312	27.540998033318793	22.364111511589645
36	23.07732041598751	27.208307506294144	27.192428659978418	22.521943417739923
37	22.834354936216776	26.951950228425815	27.40880185495535	22.804892980402062
38	22.784231349051478	27.27507518538075	27.40880185495535	22.531891610612426
39	22.353589384512958	27.274118628373778	27.694238465835607	22.678053521277654
40	22.43145312488043	27.486282972520033	27.77401532021702	22.308248582382515
41	22.586415360009795	26.76790866028452	27.676829128308732	22.968846851396957
42	22.287778262433328	27.572947037351636	27.65004553211353	22.489229168101502
43	22.81158887945086	26.888052220360127	27.511918700306865	22.78844019988215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	25.0
2	35.0
3	98.0
4	161.0
5	161.0
6	199.5
7	238.0
8	224.0
9	210.0
10	286.5
11	363.0
12	363.0
13	618.0
14	873.0
15	1563.5
16	2254.0
17	2694.0
18	3134.0
19	3134.0
20	3432.5
21	3731.0
22	4550.0
23	5369.0
24	6878.5
25	8388.0
26	8388.0
27	10612.5
28	12837.0
29	15297.5
30	17758.0
31	20466.0
32	23174.0
33	23174.0
34	26598.0
35	30022.0
36	32285.0
37	34548.0
38	36704.5
39	38861.0
40	38861.0
41	40174.0
42	41487.0
43	41543.0
44	41599.0
45	41424.5
46	41250.0
47	41250.0
48	39635.5
49	38021.0
50	37101.0
51	36181.0
52	33930.0
53	31679.0
54	31679.0
55	29961.5
56	28244.0
57	25140.5
58	22037.0
59	20174.0
60	18311.0
61	18311.0
62	16164.5
63	14018.0
64	11818.5
65	9619.0
66	8177.5
67	6736.0
68	6736.0
69	5602.0
70	4468.0
71	3699.5
72	2931.0
73	2337.5
74	1744.0
75	1744.0
76	1386.5
77	1029.0
78	804.5
79	580.0
80	465.5
81	351.0
82	351.0
83	288.0
84	225.0
85	174.0
86	123.0
87	99.0
88	75.0
89	75.0
90	49.5
91	24.0
92	14.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	522708.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.01815844690303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.66621210086603	65.62909736168614
2	7.089316537461474	10.494763095001467
3	1.7297762575015072	3.841045593163124
4	0.7595468448055623	2.248810348266535
5	0.40841764553953874	1.5115161000028328
6	0.25289976495598215	1.1231504924217852
7	0.1854299221456883	0.9607626950723517
8	0.13433967945198716	0.7954860543506679
9	0.11218323533599751	0.7473236839367466
>10	0.6043398871390029	8.32789767955142
>50	0.04113585846888404	2.0022452439975873
>100	0.015621206026957863	1.7374626953265173
>500	2.6035343378247376E-4	0.11119298748924004
>1k	5.207068675649475E-4	0.46924596973362137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1358	0.25980088309342886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1077	0.20604237930163688	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	577	0.1103866786044981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.913114013942775E-4	0.0	0.0
5	0.0	0.0	1.913114013942775E-4	0.0	0.0
6	0.0	0.0	1.913114013942775E-4	1.913114013942775E-4	0.0
7	0.0	0.0	0.00153049121115422	1.913114013942775E-4	0.0
8	0.0	0.0	0.0022957368167313297	1.913114013942775E-4	0.0
9	0.0	0.0	0.002678359619519885	9.565570069713875E-4	0.0
10	1.913114013942775E-4	0.0	0.0036349166264912722	0.0011478684083656649	0.0
11	1.913114013942775E-4	0.0	0.00612196484461688	0.0011478684083656649	0.0
12	1.913114013942775E-4	0.0	0.00688721045019399	0.0011478684083656649	0.0
13	1.913114013942775E-4	0.0	0.007843767457165376	0.0011478684083656649	0.0
14	1.913114013942775E-4	0.0	0.008991635865531042	0.0011478684083656649	0.0
15	1.913114013942775E-4	0.0	0.009756881471108153	0.0011478684083656649	0.0
16	1.913114013942775E-4	0.0	0.010522127076685261	0.0011478684083656649	0.0
17	1.913114013942775E-4	0.0	0.01071343847807954	0.0013391798097599425	0.0
18	1.913114013942775E-4	0.0	0.011096061280868095	0.0013391798097599425	0.0
19	1.913114013942775E-4	0.0	0.011096061280868095	0.0013391798097599425	0.0
20	1.913114013942775E-4	0.0	0.011287372682262372	0.0013391798097599425	0.0
21	1.913114013942775E-4	0.0	0.01147868408365665	0.0013391798097599425	0.0
22	1.913114013942775E-4	0.0	0.011669995485050927	0.0013391798097599425	0.0
23	1.913114013942775E-4	0.0	0.011669995485050927	0.00153049121115422	0.0
24	1.913114013942775E-4	0.0	0.011669995485050927	0.0022957368167313297	0.0
25	1.913114013942775E-4	0.0	0.011861306886445204	0.0022957368167313297	0.0
26	1.913114013942775E-4	0.0	0.011861306886445204	0.0024870482181256074	0.0
27	1.913114013942775E-4	0.0	0.011861306886445204	0.0032522938237027174	0.0
28	1.913114013942775E-4	0.0	0.011861306886445204	0.010904749879473817	0.0
29	1.913114013942775E-4	0.0	0.011861306886445204	0.02448785937846752	0.0
30	1.913114013942775E-4	0.0	0.011861306886445204	0.04094063989837538	0.0
31	1.913114013942775E-4	0.0	0.011861306886445204	0.08895980164833904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	260	0.0	27.038462	1
ATACTGC	90	9.45347E-8	20.555555	6
GTATCAA	610	0.0	19.409836	1
CCTAATA	60	9.2276325E-4	18.5	2
CTAACCG	85	2.7185188E-5	17.411764	6
AAAGCGT	75	2.0645499E-4	17.266666	35
GGCGTCA	65	0.0015783764	17.076923	25
GGCCGTT	65	0.0015783764	17.076923	14
CCGTTAA	65	0.0015783764	17.076923	16
ACCGACC	90	4.4385E-5	16.444445	8
CCGTGCA	90	4.4385E-5	16.444445	10
TAACCGT	90	4.4385E-5	16.444445	7
TATACTG	125	1.6525155E-7	16.279999	5
TCTATAC	115	1.2389519E-6	16.086956	3
GCCGTTA	70	0.0025899594	15.857142	15
AACCGTG	95	7.0479065E-5	15.578948	8
ATAAGAC	95	7.0479065E-5	15.578948	3
ATAACAC	85	5.357205E-4	15.235294	3
TCCGGTC	85	5.357205E-4	15.235294	23
TAAAGAC	100	1.091553E-4	14.8	3
>>END_MODULE