FastQCFastQC Report
Fri 10 Feb 2017
ERR1633225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633797
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42470.6700883721443933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.5116780294005809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16790.2649113201861164No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10850.1711904600368888No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10170.16046147267973815No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9790.15446586209780103No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8970.14152796557888409No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.13821460183623463No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8740.13789904338455372No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8580.13537457577110654No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7870.12417225073643454No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7710.12164778312298732No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7430.11722996479945473No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6850.1080787697007086No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6820.10760543202318724No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6560.10350317215133552No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6510.10271427602213327No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6460.10192537989293102No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6410.10113648376372877No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6360.10034758763452652No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6340.10003202918284562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA200.001841313237.033
GTTACGG551.900913E-523.54545417
CGCCGTT551.900913E-523.54545425
GGTATCA7900.023.1835441
ATCCGTA400.001930236723.12512
TCGACTA400.001930236723.12519
GGACCGT753.7352947E-722.26
CTCTATA502.7002487E-422.21
AAGGGTT855.1715688E-821.7647065
TACGGCC603.722892E-521.58333219
CCGGCAG957.1304385E-921.42105316
AACGATT705.0959643E-621.14285922
TGCGGTA806.9499765E-720.812536
GGTCGTG450.003823943720.55555511
TTTACCG450.003823943720.55555530
GTCAAGC450.003823943720.55555530
TTGCGGT909.4614734E-820.55555535
CGCACTA450.003823943720.55555512
GTATCAA18550.020.5444741
CGCTCTC1052.2546374E-819.3809529
Produced by FastQC (version 0.11.5)