##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633797
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846616503391463	33.0	33.0	33.0	27.0	33.0
2	31.957185029275934	33.0	33.0	33.0	33.0	33.0
3	31.678800940995302	33.0	33.0	33.0	27.0	33.0
4	31.969043715890102	33.0	33.0	33.0	33.0	33.0
5	32.069943530815074	33.0	33.0	33.0	33.0	33.0
6	35.210633688704746	37.0	37.0	37.0	33.0	37.0
7	35.35607457908447	37.0	37.0	37.0	33.0	37.0
8	35.51432714260244	37.0	37.0	37.0	33.0	37.0
9	35.627980252352096	37.0	37.0	37.0	33.0	37.0
10	35.62123361265515	37.0	37.0	37.0	33.0	37.0
11	35.63997620689274	37.0	37.0	37.0	33.0	37.0
12	35.62450595379909	37.0	37.0	37.0	33.0	37.0
13	35.639390845964876	37.0	37.0	37.0	33.0	37.0
14	35.604322835229574	37.0	37.0	37.0	33.0	37.0
15	35.63751958434641	37.0	37.0	37.0	33.0	37.0
16	35.61377381085742	37.0	37.0	37.0	33.0	37.0
17	35.6193733955825	37.0	37.0	37.0	33.0	37.0
18	35.6020271474936	37.0	37.0	37.0	33.0	37.0
19	35.590561331151775	37.0	37.0	37.0	33.0	37.0
20	35.59381947216538	37.0	37.0	37.0	33.0	37.0
21	35.598797406740644	37.0	37.0	37.0	33.0	37.0
22	35.495554570311945	37.0	37.0	37.0	33.0	37.0
23	35.552781095524274	37.0	37.0	37.0	33.0	37.0
24	35.530510557796894	37.0	37.0	37.0	33.0	37.0
25	35.5582386789461	37.0	37.0	37.0	33.0	37.0
26	35.450518068088044	37.0	37.0	37.0	33.0	37.0
27	35.462398843793835	37.0	37.0	37.0	33.0	37.0
28	35.48278234198016	37.0	37.0	37.0	33.0	37.0
29	35.482888054061476	37.0	37.0	37.0	33.0	37.0
30	35.477509360252576	37.0	37.0	37.0	33.0	37.0
31	35.472110155144314	37.0	37.0	37.0	33.0	37.0
32	35.46012051177269	37.0	37.0	37.0	33.0	37.0
33	35.45270173257368	37.0	37.0	37.0	33.0	37.0
34	35.42773159229217	37.0	37.0	37.0	33.0	37.0
35	35.35339864341422	37.0	37.0	37.0	33.0	37.0
36	35.37355020613856	37.0	37.0	37.0	33.0	37.0
37	35.369206544051174	37.0	37.0	37.0	33.0	37.0
38	35.35110768905501	37.0	37.0	37.0	33.0	37.0
39	35.24099672292548	37.0	37.0	37.0	33.0	37.0
40	35.03284963481998	37.0	37.0	37.0	27.0	37.0
41	35.18861244215419	37.0	37.0	37.0	33.0	37.0
42	35.21988112913125	37.0	37.0	37.0	33.0	37.0
43	34.880296056939365	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	18.0
16	39.0
17	14.0
18	16.0
19	19.0
20	39.0
21	133.0
22	276.0
23	684.0
24	1389.0
25	2553.0
26	4123.0
27	6421.0
28	8848.0
29	11987.0
30	15816.0
31	20211.0
32	25665.0
33	34066.0
34	48360.0
35	97684.0
36	355436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28924561018749	20.85636252617163	13.646956359843927	24.207435503796955
2	15.967730992731111	21.71184148867855	38.97572882168897	23.34469869690137
3	17.415039831365565	27.97031226086586	30.176065838115356	24.43858206965322
4	12.073266361311271	17.95622257599831	40.36513268443997	29.605378378250453
5	13.519944083042363	37.6781524683771	35.280381573279776	13.521521875300765
6	28.756052805551302	40.566143418160706	17.355872621675395	13.321931154612596
7	26.216753944875094	32.74912945312142	23.55359839191413	17.48051821008935
8	24.54082300799783	35.51405260674948	20.68596096226394	19.25916342298875
9	26.251465374559995	15.005751052781886	20.451658811890873	38.29112476076725
10	15.417081494547938	27.796439553989682	33.64152875447501	23.144950196987363
11	33.312401289371834	23.88966814295429	24.282065077619492	18.515865490054388
12	23.195439549256307	26.255252075980163	29.821693696877706	20.72761467788582
13	27.415402723584997	22.556591463828322	26.451529432925685	23.576476379660996
14	21.84721606444966	21.13326506752162	28.546048655957662	28.473470212071057
15	24.17035738572445	28.348666844431257	25.483080544717	21.99789522512729
16	22.466499525873427	28.538475253117323	25.466198167552072	23.528827053457178
17	21.92468566433732	27.664693900412907	26.65979801103508	23.750822424214693
18	22.387767692179043	27.276872563297083	28.55220204576544	21.783157698758433
19	24.53451183896421	26.199556009258483	28.170691877683236	21.09524027409407
20	23.64148142070726	26.17399577467233	28.46495013387567	21.719572670744732
21	22.756971080645698	27.120197792037516	28.364444767015307	21.758386360301486
22	22.46492173361502	26.937647227740115	28.107580187347054	22.489850851297813
23	22.365678600561377	26.763774520863937	28.732859259352754	22.13768761922193
24	22.89518568248193	27.373749007963116	27.072390686607857	22.658674622947096
25	22.543180229631883	27.064028387638313	28.47094574445761	21.921845638272192
26	22.830022862209827	27.17841832637264	28.40641404108887	21.58514477032867
27	22.908123579000847	27.05976834854062	27.39189361893477	22.640214453523765
28	21.87940302652111	26.77213681983348	28.882276186223667	22.466183967421745
29	23.890930376761013	26.480087472802804	27.627458003114562	22.001524147321618
30	23.137219014921182	26.26487660875643	28.56308881234843	22.034815563973954
31	22.414116822894396	26.786021391707436	28.37974935192183	22.420112433476334
32	21.38539627041466	26.56229044946568	28.720710258963045	23.331603021156617
33	21.699850267514677	26.76125005325049	29.027433073996878	22.511466605237953
34	22.509415475302028	26.745629909892283	28.77703744258808	21.967917172217604
35	22.610236400614074	27.232221042384236	27.938756415697767	22.21878614130392
36	23.443941829955016	26.9758298003935	27.57081526103784	22.00941310861364
37	22.48874639671693	26.587219567148473	27.809219671282758	23.11481436485184
38	22.954037333720418	26.90735361637875	27.694987511774276	22.44362153812656
39	22.042073408362615	26.94474729290293	28.16832518929563	22.844854109438828
40	22.374356457982604	27.53799718206303	28.38590274172961	21.701743618224764
41	22.168296789034976	26.3852621580727	28.327524428168644	23.11891662472369
42	21.7779509843057	27.74200572107473	27.963527754154722	22.516515540464848
43	22.78726469200706	26.034992276706898	28.046046289269277	23.131696742016768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	41.5
2	58.0
3	266.5
4	475.0
5	475.0
6	581.0
7	687.0
8	651.5
9	616.0
10	853.0
11	1090.0
12	1090.0
13	1768.5
14	2447.0
15	4200.0
16	5953.0
17	6497.5
18	7042.0
19	7042.0
20	6488.5
21	5935.0
22	5921.0
23	5907.0
24	7497.0
25	9087.0
26	9087.0
27	11338.5
28	13590.0
29	15659.5
30	17729.0
31	21242.0
32	24755.0
33	24755.0
34	29775.0
35	34795.0
36	37503.5
37	40212.0
38	42883.5
39	45555.0
40	45555.0
41	47175.5
42	48796.0
43	49964.5
44	51133.0
45	49456.0
46	47779.0
47	47779.0
48	45988.0
49	44197.0
50	44610.5
51	45024.0
52	42867.5
53	40711.0
54	40711.0
55	39801.5
56	38892.0
57	33342.5
58	27793.0
59	24999.5
60	22206.0
61	22206.0
62	19789.0
63	17372.0
64	13992.5
65	10613.0
66	8866.5
67	7120.0
68	7120.0
69	5907.5
70	4695.0
71	4092.5
72	3490.0
73	3498.5
74	3507.0
75	3507.0
76	3036.5
77	2566.0
78	1875.5
79	1185.0
80	756.5
81	328.0
82	328.0
83	249.5
84	171.0
85	157.0
86	143.0
87	105.5
88	68.0
89	68.0
90	55.5
91	43.0
92	24.0
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	633797.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.13882894782177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14762371680769	47.18070293613387
2	7.313256111642469	7.918622433596474
3	1.8884504879769888	3.0671549383505026
4	0.8470596037872405	1.8343525999218835
5	0.5123975955432407	1.3870302889195334
6	0.34583879500364384	1.1233984419733842
7	0.25269653701868633	0.95764862153531
8	0.18593697224925437	0.8053127948542613
9	0.14949141733169363	0.72839612448792
>10	1.0696728165671263	11.664186011693465
>50	0.15355135728956756	5.875064528941127
>100	0.12544464822016663	12.981715367544222
>500	0.007100640465292402	2.671080798415322
>1k	0.001479300096935917	1.8053341136327112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4247	0.6700883721443933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3243	0.5116780294005809	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.2649113201861164	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1085	0.1711904600368888	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1017	0.16046147267973815	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	979	0.15446586209780103	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	897	0.14152796557888409	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	876	0.13821460183623463	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	874	0.13789904338455372	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	858	0.13537457577110654	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	787	0.12417225073643454	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	771	0.12164778312298732	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	743	0.11722996479945473	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	685	0.1080787697007086	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	682	0.10760543202318724	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	656	0.10350317215133552	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	651	0.10271427602213327	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	646	0.10192537989293102	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	641	0.10113648376372877	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	636	0.10034758763452652	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	634	0.10003202918284562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.577792258404505E-4	1.577792258404505E-4	0.0
2	3.15558451680901E-4	0.0	1.577792258404505E-4	1.577792258404505E-4	0.0
3	3.15558451680901E-4	0.0	4.7333767752135147E-4	1.577792258404505E-4	0.0
4	3.15558451680901E-4	0.0	4.7333767752135147E-4	3.15558451680901E-4	0.0
5	3.15558451680901E-4	0.0	4.7333767752135147E-4	4.7333767752135147E-4	0.0
6	3.15558451680901E-4	0.0	0.001262233806723604	4.7333767752135147E-4	1.577792258404505E-4
7	3.15558451680901E-4	0.0	0.0036289221943303614	6.31116903361802E-4	1.577792258404505E-4
8	3.15558451680901E-4	0.0	0.004575597549373064	6.31116903361802E-4	1.577792258404505E-4
9	3.15558451680901E-4	0.0	0.005364493678575316	0.001577792258404505	1.577792258404505E-4
10	6.31116903361802E-4	0.0	0.006153389807777569	0.0017355714842449553	1.577792258404505E-4
11	7.888961292022525E-4	0.0	0.00994009122794838	0.0018933507100854059	1.577792258404505E-4
12	9.466753550427029E-4	0.0	0.010886766582991083	0.0018933507100854059	1.577792258404505E-4
13	9.466753550427029E-4	0.0	0.012306779615555138	0.0018933507100854059	1.577792258404505E-4
14	9.466753550427029E-4	0.0	0.012780117293076489	0.0018933507100854059	1.577792258404505E-4
15	9.466753550427029E-4	0.0	0.014515688777321446	0.0018933507100854059	1.577792258404505E-4
16	9.466753550427029E-4	0.0	0.014673468003161896	0.0022089091617663067	1.577792258404505E-4
17	9.466753550427029E-4	0.0	0.015146805680683247	0.002524467613447208	1.577792258404505E-4
18	9.466753550427029E-4	0.0	0.015304584906523698	0.002682246839287658	1.577792258404505E-4
19	9.466753550427029E-4	0.0	0.015304584906523698	0.002840026065128109	1.577792258404505E-4
20	9.466753550427029E-4	0.0	0.0156201433582046	0.0029978052909685592	1.577792258404505E-4
21	9.466753550427029E-4	0.0	0.01640903948740685	0.00315558451680901	3.15558451680901E-4
22	9.466753550427029E-4	0.0	0.01640903948740685	0.0037867014201708117	3.15558451680901E-4
23	9.466753550427029E-4	0.0	0.016566818713247302	0.003944480646011262	3.15558451680901E-4
24	9.466753550427029E-4	0.0	0.016724597939087753	0.004575597549373064	3.15558451680901E-4
25	0.0011044545808831534	0.0	0.016882377164928204	0.004575597549373064	3.15558451680901E-4
26	0.0011044545808831534	0.0	0.016882377164928204	0.005522272904415767	3.15558451680901E-4
27	0.0011044545808831534	0.0	0.016882377164928204	0.006942285936979821	3.15558451680901E-4
28	0.0011044545808831534	0.0	0.016882377164928204	0.020353520133418113	3.15558451680901E-4
29	0.0011044545808831534	0.0	0.016882377164928204	0.046544871622932894	3.15558451680901E-4
30	0.0011044545808831534	0.0	0.016882377164928204	0.07888961292022524	3.15558451680901E-4
31	0.0011044545808831534	0.0	0.016882377164928204	0.1434213162889695	3.15558451680901E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGATA	20	0.0018413132	37.0	33
GTTACGG	55	1.900913E-5	23.545454	17
CGCCGTT	55	1.900913E-5	23.545454	25
GGTATCA	790	0.0	23.183544	1
ATCCGTA	40	0.0019302367	23.125	12
TCGACTA	40	0.0019302367	23.125	19
GGACCGT	75	3.7352947E-7	22.2	6
CTCTATA	50	2.7002487E-4	22.2	1
AAGGGTT	85	5.1715688E-8	21.764706	5
TACGGCC	60	3.722892E-5	21.583332	19
CCGGCAG	95	7.1304385E-9	21.421053	16
AACGATT	70	5.0959643E-6	21.142859	22
TGCGGTA	80	6.9499765E-7	20.8125	36
GGTCGTG	45	0.0038239437	20.555555	11
TTTACCG	45	0.0038239437	20.555555	30
GTCAAGC	45	0.0038239437	20.555555	30
TTGCGGT	90	9.4614734E-8	20.555555	35
CGCACTA	45	0.0038239437	20.555555	12
GTATCAA	1855	0.0	20.544474	1
CGCTCTC	105	2.2546374E-8	19.38095	29
>>END_MODULE