FastQCFastQC Report
Fri 10 Feb 2017
ERR1633224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350529
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12720.36288010407127513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.28100385417469026No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT9320.26588384983838714No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8820.25161969480413887No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8370.23878195527331547No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7320.20882722970139417No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6530.18628986474728196No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC6460.1842928830424872No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA6450.18400759994180227No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6200.17687552242467813No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT6190.17659023932399318No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5950.169743444907554No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG5720.1631819335917998No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5670.161755518088375No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5310.15148532646371626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5270.1503441940609764No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4490.12809211220754915No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG4470.12752154600617924No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT4460.12723626290549428No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4410.12580984740206944No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG4360.12438343189864463No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC4320.12324229949590476No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4270.12181588399247995No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA4240.12096003469042504No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA4210.12010418538837014No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT4080.11639550507946561No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC3990.11382795717330094No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA3970.113257390971931No Hit
ACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGG3610.10298719934727227No Hit
TTTTAAAGCTGATATTTTAGCAATATTTTGATAAAACTTTATT3610.10298719934727227No Hit
ATGTAAACAGGTTGGGGTACTTCATCCAAAATAAGAAGAAAAT3590.10241663314590234No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG3550.10127550074316247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG250.005491197629.5999981
ACGTTGA405.9285332E-527.75000231
GGTATCA2500.027.3800011
CCGAATT358.857048E-426.42857214
GGCAGCG400.001928524123.12500237
CGAATTA400.001928524123.12500215
TCGGCCT1201.0913936E-1121.58333423
CTCGGCC1251.8189894E-1120.72000122
GGCCGTT450.003820583720.55555514
CATACTT555.1332964E-420.1818185
TTAACGG555.1332964E-420.18181835
GTATGTA656.884652E-519.9230771
GTATCAA5550.019.6666661
CCTCGGC1254.0927262E-1019.24000221
TAATATA902.1435444E-618.54
CTAGAAC801.6114516E-518.53
ATAATAT801.6114516E-518.53
AACGGCC609.2193653E-418.537
TTATTCT609.2193653E-418.54
TTCCTCG1306.91216E-1018.519
Produced by FastQC (version 0.11.5)