##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350529
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.034445081576703	33.0	33.0	33.0	27.0	33.0
2	31.38562287285788	33.0	33.0	33.0	27.0	33.0
3	31.138168311323742	33.0	33.0	33.0	27.0	33.0
4	31.457964961529573	33.0	33.0	33.0	27.0	33.0
5	31.58421129207569	33.0	33.0	33.0	27.0	33.0
6	34.22459767950726	37.0	33.0	37.0	27.0	37.0
7	34.40809747552984	37.0	37.0	37.0	27.0	37.0
8	34.534874432643235	37.0	37.0	37.0	27.0	37.0
9	34.650222948743185	37.0	37.0	37.0	27.0	37.0
10	34.68067977257231	37.0	37.0	37.0	27.0	37.0
11	34.639539096622535	37.0	37.0	37.0	27.0	37.0
12	34.72831919755569	37.0	37.0	37.0	27.0	37.0
13	34.75374648031975	37.0	37.0	37.0	27.0	37.0
14	34.724530637978596	37.0	37.0	37.0	27.0	37.0
15	34.738734883561705	37.0	37.0	37.0	27.0	37.0
16	34.70248395995766	37.0	37.0	37.0	27.0	37.0
17	34.70588453451783	37.0	37.0	37.0	27.0	37.0
18	34.689823095949265	37.0	37.0	37.0	27.0	37.0
19	34.67949299487346	37.0	37.0	37.0	27.0	37.0
20	34.6750910766299	37.0	37.0	37.0	27.0	37.0
21	34.69686388287417	37.0	37.0	37.0	27.0	37.0
22	34.58646788140211	37.0	37.0	37.0	27.0	37.0
23	34.629023561531284	37.0	37.0	37.0	27.0	37.0
24	34.60467179605682	37.0	37.0	37.0	27.0	37.0
25	34.615261504754244	37.0	37.0	37.0	27.0	37.0
26	34.48695257739017	37.0	37.0	37.0	27.0	37.0
27	34.51220869029381	37.0	37.0	37.0	27.0	37.0
28	34.513709279403415	37.0	37.0	37.0	27.0	37.0
29	34.539872592567235	37.0	37.0	37.0	27.0	37.0
30	34.5378157014113	37.0	37.0	37.0	27.0	37.0
31	34.54434297875497	37.0	37.0	37.0	27.0	37.0
32	34.51279066781921	37.0	37.0	37.0	27.0	37.0
33	34.49007928017368	37.0	37.0	37.0	27.0	37.0
34	34.47617172901529	37.0	37.0	37.0	27.0	37.0
35	34.421876649292926	37.0	37.0	37.0	27.0	37.0
36	34.450598951869885	37.0	37.0	37.0	27.0	37.0
37	34.458755195718474	37.0	37.0	37.0	27.0	37.0
38	34.43660296295028	37.0	37.0	37.0	27.0	37.0
39	34.35132613849353	37.0	37.0	37.0	27.0	37.0
40	34.129644052275275	37.0	37.0	37.0	27.0	37.0
41	34.324058779730066	37.0	37.0	37.0	27.0	37.0
42	34.35738840438309	37.0	37.0	37.0	27.0	37.0
43	33.905862282435976	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	9.0
16	23.0
17	18.0
18	21.0
19	32.0
20	96.0
21	271.0
22	528.0
23	1156.0
24	1985.0
25	3090.0
26	4785.0
27	6347.0
28	8551.0
29	10905.0
30	13661.0
31	16699.0
32	20726.0
33	26757.0
34	38619.0
35	68646.0
36	127600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54166417043953	22.661463102910172	14.183134633653708	23.613738092996588
2	15.473755381152488	23.534429391006164	38.579689554929836	22.412125672911515
3	17.452478967503403	28.30236585275398	31.953989541521526	22.291165638221088
4	13.085650545318646	19.507087858636517	39.003334959447	28.403926636597827
5	13.403741202582383	38.84186472445932	35.589066810449346	12.16532726250895
6	28.004245012538192	40.93384570178216	18.043300269021962	13.018609016657681
7	24.068764638589105	34.0074573002519	24.633625177945333	17.290152883213658
8	23.137885881054064	37.51187490906601	21.30922120566344	18.041018004216482
9	24.594256110050807	16.529873419888226	20.743504817005153	38.132365653055814
10	13.793152635017359	29.65460775000071	35.04217910643627	21.510060508545656
11	31.746303444222875	24.906070539099474	25.494324292711873	17.853301723965778
12	20.924089019738737	27.29788405524222	31.001714551435118	20.776312373583927
13	25.938795363579047	23.297359134336958	28.302936418955348	22.460909083128644
14	20.7312376436757	22.55619363875742	28.456133444023173	28.2564352735437
15	22.85146164796636	30.187801865180912	25.57990922291736	21.380827263935366
16	22.016438012261467	30.51530686476725	25.433273709165288	22.03498141380599
17	21.524895229781276	29.20585743262326	26.993201703710678	22.276045633884785
18	20.585457979225687	29.286877833217794	28.81673128328897	21.31093290426755
19	23.193230802586946	27.941197447286815	29.22012158765751	19.645450162468727
20	23.039177928217068	27.125573062428497	29.46945901765617	20.36578999169826
21	20.959464124223672	29.29458047693629	27.97086688975805	21.77508850908199
22	20.893278444864762	28.873217337224595	28.206796014024516	22.026708203886123
23	20.988277717392855	28.75625126594376	28.49379081331359	21.761680203349794
24	22.61524724059921	29.055227955461604	27.37662219103127	20.95290261290792
25	22.40385246299165	27.711829834336104	28.59078706754648	21.29353063512577
26	21.696350373292937	28.172847325043005	28.17085034333821	21.959951958325842
27	21.568258261085386	27.986842743396412	28.067007294688885	22.37789170082932
28	20.540383249317458	28.661251993415664	28.966790194249263	21.83157456301761
29	22.174199566940253	28.99874190152598	27.713541532940212	21.113516998593553
30	21.262434777151107	29.216698190449293	28.098673718864912	21.422193313534684
31	22.052954249149142	28.269273013074525	28.59963084366771	21.07814189410862
32	20.945485252290112	28.424467019847143	28.687212755577995	21.942834972284746
33	21.13691021284972	28.05930465097039	28.9034573458972	21.900327790282688
34	21.082991706820266	28.463836087741672	28.306930382364943	22.146241823073126
35	21.717461322743624	28.933126788368433	27.854756667779274	21.494655221108665
36	22.125416156723123	28.762242211058144	27.406291633502505	21.706049998716225
37	22.61296497579373	27.265361781764135	27.73351134998816	22.388161892453976
38	22.342231313243698	28.623309341024566	27.868164973511465	21.166294372220275
39	21.625600164323068	28.449571932707425	28.272125844081376	21.65270205888814
40	21.91088326500803	28.094679755455328	28.29979830484782	21.694638674688825
41	21.181699659657262	28.687212755577995	28.52174855718072	21.609339027584024
42	21.97706894436694	28.210219411232735	28.01052124075326	21.80219040364706
43	21.72402283405938	27.906678192103936	28.284393017410824	22.08490595642586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	19.0
2	22.0
3	80.0
4	138.0
5	138.0
6	176.0
7	214.0
8	203.0
9	192.0
10	281.0
11	370.0
12	370.0
13	608.0
14	846.0
15	1763.5
16	2681.0
17	3022.5
18	3364.0
19	3364.0
20	3483.0
21	3602.0
22	4612.0
23	5622.0
24	6632.5
25	7643.0
26	7643.0
27	8834.5
28	10026.0
29	12972.0
30	15918.0
31	16355.5
32	16793.0
33	16793.0
34	18793.5
35	20794.0
36	21507.5
37	22221.0
38	24817.5
39	27414.0
40	27414.0
41	29215.5
42	31017.0
43	28194.0
44	25371.0
45	25863.5
46	26356.0
47	26356.0
48	25597.5
49	24839.0
50	25067.0
51	25295.0
52	25351.5
53	25408.0
54	25408.0
55	20903.0
56	16398.0
57	13902.0
58	11406.0
59	9898.5
60	8391.0
61	8391.0
62	7354.5
63	6318.0
64	5203.0
65	4088.0
66	3401.0
67	2714.0
68	2714.0
69	2300.0
70	1886.0
71	1589.0
72	1292.0
73	1049.0
74	806.0
75	806.0
76	613.0
77	420.0
78	337.0
79	254.0
80	199.0
81	144.0
82	144.0
83	125.5
84	107.0
85	89.5
86	72.0
87	61.0
88	50.0
89	50.0
90	34.0
91	18.0
92	10.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	350529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.412505831761756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96756101377709	46.1060030464045
2	6.219373340818655	6.519458829911227
3	1.7495314404189115	2.7509198047142025
4	0.874197786108626	1.8327558625012632
5	0.5243536580351734	1.3741344579837071
6	0.36450943278902276	1.1462911663072097
7	0.28604497412091806	1.0494633711981132
8	0.20937098523149753	0.8778926387558055
9	0.16066841607688861	0.7578930865148864
>10	1.3124356984065382	14.448089693753237
>50	0.1848502235019235	6.922159808538467
>100	0.13822133829423108	12.83561383125805
>500	0.008326586644230789	3.009242556309405
>1k	5.551057762820526E-4	0.37008184584990456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1272	0.36288010407127513	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.28100385417469026	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	932	0.26588384983838714	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	882	0.25161969480413887	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	837	0.23878195527331547	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	732	0.20882722970139417	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	653	0.18628986474728196	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	646	0.1842928830424872	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	645	0.18400759994180227	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	620	0.17687552242467813	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	619	0.17659023932399318	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	595	0.169743444907554	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	572	0.1631819335917998	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	567	0.161755518088375	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	531	0.15148532646371626	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	527	0.1503441940609764	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	449	0.12809211220754915	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	447	0.12752154600617924	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	446	0.12723626290549428	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	441	0.12580984740206944	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	436	0.12438343189864463	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	432	0.12324229949590476	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	427	0.12181588399247995	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	424	0.12096003469042504	No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA	421	0.12010418538837014	No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT	408	0.11639550507946561	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	399	0.11382795717330094	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	397	0.113257390971931	No Hit
ACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGG	361	0.10298719934727227	No Hit
TTTTAAAGCTGATATTTTAGCAATATTTTGATAAAACTTTATT	361	0.10298719934727227	No Hit
ATGTAAACAGGTTGGGGTACTTCATCCAAAATAAGAAGAAAAT	359	0.10241663314590234	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	355	0.10127550074316247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	2.852831006849647E-4	0.0	0.0
7	0.0	0.0	5.705662013699294E-4	0.0	0.0
8	0.0	0.0	5.705662013699294E-4	0.0	0.0
9	0.0	0.0	0.0014264155034248236	0.0	0.0
10	0.0	0.0	0.001996981704794753	0.0	0.0
11	0.0	0.0	0.0025675479061646827	0.0	0.0
12	0.0	0.0	0.0028528310068496473	0.0	0.0
13	0.0	0.0	0.0028528310068496473	0.0	0.0
14	0.0	0.0	0.0028528310068496473	0.0	0.0
15	0.0	0.0	0.003138114107534612	0.0	0.0
16	0.0	0.0	0.0037086803089045415	0.0	0.0
17	0.0	0.0	0.0037086803089045415	0.0	0.0
18	0.0	0.0	0.0037086803089045415	0.0	0.0
19	0.0	0.0	0.004279246510274471	0.0	0.0
20	0.0	0.0	0.004564529610959435	0.0	0.0
21	0.0	0.0	0.005135095812329365	2.852831006849647E-4	0.0
22	0.0	0.0	0.00542037891301433	8.558493020548942E-4	0.0
23	0.0	0.0	0.00542037891301433	0.0011411324027398588	0.0
24	0.0	0.0	0.00542037891301433	0.0017116986041097884	0.0
25	0.0	0.0	0.00542037891301433	0.0028528310068496473	0.0
26	0.0	0.0	0.00542037891301433	0.0028528310068496473	0.0
27	0.0	0.0	0.0057056620136992945	0.004279246510274471	0.0
28	0.0	0.0	0.0057056620136992945	0.009699625423288801	0.0
29	0.0	0.0	0.0057056620136992945	0.019684533947262568	0.0
30	0.0	0.0	0.0057056620136992945	0.03309283967945591	0.0
31	0.0	0.0	0.0057056620136992945	0.07560002168151565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTAG	25	0.0054911976	29.599998	1
ACGTTGA	40	5.9285332E-5	27.750002	31
GGTATCA	250	0.0	27.380001	1
CCGAATT	35	8.857048E-4	26.428572	14
GGCAGCG	40	0.0019285241	23.125002	37
CGAATTA	40	0.0019285241	23.125002	15
TCGGCCT	120	1.0913936E-11	21.583334	23
CTCGGCC	125	1.8189894E-11	20.720001	22
GGCCGTT	45	0.0038205837	20.555555	14
CATACTT	55	5.1332964E-4	20.181818	5
TTAACGG	55	5.1332964E-4	20.181818	35
GTATGTA	65	6.884652E-5	19.923077	1
GTATCAA	555	0.0	19.666666	1
CCTCGGC	125	4.0927262E-10	19.240002	21
TAATATA	90	2.1435444E-6	18.5	4
CTAGAAC	80	1.6114516E-5	18.5	3
ATAATAT	80	1.6114516E-5	18.5	3
AACGGCC	60	9.2193653E-4	18.5	37
TTATTCT	60	9.2193653E-4	18.5	4
TTCCTCG	130	6.91216E-10	18.5	19
>>END_MODULE