FastQCFastQC Report
Fri 10 Feb 2017
ERR1633223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413230
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15210.368075889940227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.282167316022554No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8550.20690656535101515No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7830.1894828545846139No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7330.1773830554412797No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6610.15995934467487838No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6570.15899136074341166No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6430.1556034169832781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6380.15439343706894468No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6150.14882752946301092No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5700.13793771023401014No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5560.13454976647387654No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4850.11736805169034195No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4740.11470609587880842No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4670.11301212399874162No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4640.11228613605014158No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4600.11131815211867482No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4550.11010817220434141No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4480.10841420032427461No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4470.10817220434140794No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4430.1072042204099412No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4420.1069622244270745No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4410.10672022844420784No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4300.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATG200.00184057237.02
GGTATCA3350.029.8208961
TGCGGTA405.9310645E-527.75000236
TCGGAGC405.9310645E-527.7500023
CGAATGT400.001929105523.12500215
GTATCAA8950.021.703911
GCCGCTC603.718856E-521.58333227
GTCGCCC1400.021.14285937
GCACCGC1750.021.14285910
GCACCCT450.003821723920.5555556
TATACGG450.003821723920.5555552
AGGTCGC1450.020.41379435
AACTTTG2100.020.26190437
GTATTAT555.135434E-420.1818181
GTATTAG2200.020.1818181
AGCTTCG555.135434E-420.18181821
CGCTCTC555.135434E-420.18181829
CCACATA555.135434E-420.18181813
AGGCACC1900.019.4736848
TATTAGA1900.019.4736842
Produced by FastQC (version 0.11.5)