##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413230
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.806417733465626	33.0	33.0	33.0	27.0	33.0
2	31.92559591510781	33.0	33.0	33.0	33.0	33.0
3	31.657077172518935	33.0	33.0	33.0	27.0	33.0
4	31.94048108801394	33.0	33.0	33.0	33.0	33.0
5	32.0334293250732	33.0	33.0	33.0	33.0	33.0
6	35.1956610120272	37.0	37.0	37.0	33.0	37.0
7	35.33271301696392	37.0	37.0	37.0	33.0	37.0
8	35.459344674878395	37.0	37.0	37.0	33.0	37.0
9	35.57297388863345	37.0	37.0	37.0	33.0	37.0
10	35.58240931200542	37.0	37.0	37.0	33.0	37.0
11	35.60403649299422	37.0	37.0	37.0	33.0	37.0
12	35.57783074800958	37.0	37.0	37.0	33.0	37.0
13	35.5851341867725	37.0	37.0	37.0	33.0	37.0
14	35.56202599036856	37.0	37.0	37.0	33.0	37.0
15	35.574609781477626	37.0	37.0	37.0	33.0	37.0
16	35.56189047261815	37.0	37.0	37.0	33.0	37.0
17	35.56473150545701	37.0	37.0	37.0	33.0	37.0
18	35.54991651138591	37.0	37.0	37.0	33.0	37.0
19	35.53443844832176	37.0	37.0	37.0	33.0	37.0
20	35.533864917842365	37.0	37.0	37.0	33.0	37.0
21	35.529760665972944	37.0	37.0	37.0	33.0	37.0
22	35.45059652009777	37.0	37.0	37.0	33.0	37.0
23	35.494022699223194	37.0	37.0	37.0	33.0	37.0
24	35.48560365897926	37.0	37.0	37.0	33.0	37.0
25	35.50612491832636	37.0	37.0	37.0	33.0	37.0
26	35.39702344941074	37.0	37.0	37.0	33.0	37.0
27	35.41010575224451	37.0	37.0	37.0	33.0	37.0
28	35.44021247247296	37.0	37.0	37.0	33.0	37.0
29	35.43153691648719	37.0	37.0	37.0	33.0	37.0
30	35.43040921520703	37.0	37.0	37.0	33.0	37.0
31	35.41769474626721	37.0	37.0	37.0	33.0	37.0
32	35.420509159547954	37.0	37.0	37.0	33.0	37.0
33	35.42279844154587	37.0	37.0	37.0	33.0	37.0
34	35.405014156765	37.0	37.0	37.0	33.0	37.0
35	35.33528059434214	37.0	37.0	37.0	33.0	37.0
36	35.361401640732765	37.0	37.0	37.0	33.0	37.0
37	35.365878566415795	37.0	37.0	37.0	33.0	37.0
38	35.351230549572875	37.0	37.0	37.0	33.0	37.0
39	35.26237688454372	37.0	37.0	37.0	33.0	37.0
40	35.05389492534424	37.0	37.0	37.0	27.0	37.0
41	35.23028337729594	37.0	37.0	37.0	33.0	37.0
42	35.279004428526484	37.0	37.0	37.0	33.0	37.0
43	34.94443772233381	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	17.0
17	21.0
18	16.0
19	9.0
20	28.0
21	82.0
22	206.0
23	451.0
24	951.0
25	1811.0
26	2829.0
27	4296.0
28	6093.0
29	8046.0
30	10462.0
31	13202.0
32	16771.0
33	22282.0
34	32226.0
35	64505.0
36	228913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38501560874089	21.250877235437894	13.70641047358614	24.657696682235077
2	16.29891343803693	21.343077704910097	38.67894392953077	23.6790649275222
3	17.078140502867655	27.745565423613964	29.948696851632263	25.227597221886118
4	12.149166323839024	18.137598915857996	40.49730174479104	29.21593301551194
5	13.32671877646831	37.70224814268083	35.9017980301527	13.069235050698158
6	28.752994700287974	40.46076035137817	17.7148319337899	13.071413014543959
7	26.53655349321201	32.2053093918641	23.843138203905813	17.414998911018074
8	24.336084021005252	35.671175858480744	20.598940057595044	19.393800062918956
9	25.81322750042349	15.404254289378796	20.4416426687317	38.34087554146601
10	15.308181884180721	27.839217868983372	33.84870411151175	23.003896135324155
11	33.38213585654478	24.147569150352105	24.157732981632503	18.31256201147061
12	23.056167267623355	26.429833264767804	29.494712387774364	21.019287079834477
13	27.102824093120052	22.490622655663916	26.252208213343657	24.15434503787237
14	21.92048012003001	21.069622244270743	27.908428720083243	29.101468915616003
15	24.049560777291095	28.78469617404351	24.784744573240083	22.380998475425308
16	23.008736054981487	28.357815260266676	25.148222539505845	23.48522614524599
17	22.2670183674951	27.959731868450984	26.040461728335313	23.732788035718606
18	22.381966459356775	27.71967185344723	27.863659463252908	22.034702223943082
19	24.854923408271425	26.213730852067858	27.2833530963386	21.64799264332212
20	23.733030031701475	26.623672047044018	27.645863078672896	21.99743484258161
21	22.97582460131162	27.350869975558407	27.502117464850084	22.17118795827989
22	22.543377779928853	27.005299712024783	27.43411659366454	23.017205914381822
23	22.50707838249885	27.04547104518065	28.067178084843793	22.380272487476706
24	22.899353870725747	27.887375069573846	25.874452484088767	23.338818575611644
25	22.89306197517121	27.232533939936598	27.581492147230357	22.292911937661835
26	22.945575103453283	27.386685381022673	27.802676475570504	21.865063039953537
27	23.031725673353822	27.270043317280933	26.363284369479466	23.33494663988578
28	22.17505989400576	26.932216925199043	28.219393558066937	22.673329622728264
29	24.310916438787117	26.62052609926675	26.802023086416764	22.266534375529364
30	23.406577450814318	26.439513104082472	27.50332744476442	22.650582000338794
31	22.872250320644678	26.772741572489895	27.56600440432689	22.789003702538537
32	21.770926602618395	26.712000580790356	27.777750889335234	23.739321927256007
33	22.02962030830288	26.886721680420106	28.226169445587203	22.85748856568981
34	23.203300825206302	26.552767224064084	27.902862812477313	22.341069138252305
35	23.059071219417756	27.331510296929075	27.09290225782252	22.516516225830653
36	24.043752873702296	26.907049342980905	26.54937928030395	22.49981850301285
37	22.808363381167872	26.389661931611936	27.164049076785325	23.637925610434866
38	23.355032306463713	27.129927643201125	26.867845993756507	22.64719405657866
39	22.286620042107298	27.06555671175859	27.60327178568836	23.044551460445756
40	22.681073494179994	27.542530793988817	27.736127580282165	22.040268131549016
41	22.39987416208891	26.353846526147667	27.71216997797837	23.534109333785057
42	22.132468601021223	27.6661907412337	27.290370979841732	22.91096967790335
43	23.245166130242236	25.851462865716428	27.57157031193282	23.331800692108512
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	22.5
2	29.0
3	83.0
4	137.0
5	137.0
6	183.0
7	229.0
8	206.0
9	183.0
10	254.5
11	326.0
12	326.0
13	522.0
14	718.0
15	1377.0
16	2036.0
17	2276.5
18	2517.0
19	2517.0
20	2389.5
21	2262.0
22	2564.5
23	2867.0
24	3797.5
25	4728.0
26	4728.0
27	6293.0
28	7858.0
29	9543.0
30	11228.0
31	13510.0
32	15792.0
33	15792.0
34	19686.0
35	23580.0
36	25695.5
37	27811.0
38	29932.5
39	32054.0
40	32054.0
41	33635.5
42	35217.0
43	35896.0
44	36575.0
45	35062.5
46	33550.0
47	33550.0
48	31727.0
49	29904.0
50	29529.5
51	29155.0
52	27646.5
53	26138.0
54	26138.0
55	25792.0
56	25446.0
57	21289.5
58	17133.0
59	15536.5
60	13940.0
61	13940.0
62	12439.5
63	10939.0
64	8733.0
65	6527.0
66	5424.5
67	4322.0
68	4322.0
69	3593.0
70	2864.0
71	2484.0
72	2104.0
73	2165.5
74	2227.0
75	2227.0
76	1896.0
77	1565.0
78	1144.0
79	723.0
80	463.0
81	203.0
82	203.0
83	163.5
84	124.0
85	114.0
86	104.0
87	81.0
88	58.0
89	58.0
90	44.0
91	30.0
92	19.0
93	8.0
94	5.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413230.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.2007739311056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78468192438604	45.30227561817782
2	6.691302507355681	6.985823389822281
3	1.9326257881518902	3.0265368558222487
4	0.9664536338830401	2.017985106288963
5	0.6007407619398856	1.5679566352612047
6	0.42429735162073445	1.3289190078912472
7	0.3033851291025078	1.108585697683751
8	0.23237752692764074	0.9704229399855342
9	0.19081186342357154	0.896447425135217
>10	1.5486127990949938	15.87231283092434
>50	0.19935069966653504	7.307999610211802
>100	0.11970467544987522	11.30272450318312
>500	0.004712782498026583	1.650979108063336
>1k	9.425564996053166E-4	0.6610312715491259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1521	0.368075889940227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1166	0.282167316022554	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	855	0.20690656535101515	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	783	0.1894828545846139	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	733	0.1773830554412797	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	661	0.15995934467487838	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	657	0.15899136074341166	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	643	0.1556034169832781	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.15439343706894468	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	615	0.14882752946301092	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	570	0.13793771023401014	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	556	0.13454976647387654	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	485	0.11736805169034195	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	474	0.11470609587880842	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	467	0.11301212399874162	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	464	0.11228613605014158	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	460	0.11131815211867482	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	455	0.11010817220434141	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	448	0.10841420032427461	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	447	0.10817220434140794	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	443	0.1072042204099412	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	442	0.1069622244270745	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	441	0.10672022844420784	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	430	0.10405827263267431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4199598286668442E-4	0.0	0.0
2	0.0	0.0	2.4199598286668442E-4	0.0	0.0
3	0.0	0.0	2.4199598286668442E-4	0.0	0.0
4	0.0	0.0	2.4199598286668442E-4	0.0	0.0
5	0.0	0.0	2.4199598286668442E-4	0.0	0.0
6	0.0	0.0	4.8399196573336884E-4	0.0	0.0
7	0.0	0.0	0.001209979914333422	0.0	0.0
8	0.0	0.0	0.001209979914333422	0.0	0.0
9	0.0	0.0	0.001693971880066791	4.8399196573336884E-4	0.0
10	2.4199598286668442E-4	0.0	0.001693971880066791	4.8399196573336884E-4	0.0
11	2.4199598286668442E-4	0.0	0.002903951794400213	4.8399196573336884E-4	0.0
12	2.4199598286668442E-4	0.0	0.003387943760133582	4.8399196573336884E-4	0.0
13	2.4199598286668442E-4	0.0	0.003387943760133582	4.8399196573336884E-4	0.0
14	2.4199598286668442E-4	0.0	0.004113931708733635	4.8399196573336884E-4	0.0
15	2.4199598286668442E-4	0.0	0.004597923674467004	4.8399196573336884E-4	0.0
16	4.8399196573336884E-4	0.0	0.004597923674467004	4.8399196573336884E-4	0.0
17	4.8399196573336884E-4	0.0	0.005081915640200373	4.8399196573336884E-4	0.0
18	4.8399196573336884E-4	0.0	0.005081915640200373	4.8399196573336884E-4	0.0
19	4.8399196573336884E-4	0.0	0.005323911623067057	4.8399196573336884E-4	0.0
20	4.8399196573336884E-4	0.0	0.005323911623067057	4.8399196573336884E-4	0.0
21	4.8399196573336884E-4	0.0	0.005565907605933742	7.259879486000532E-4	0.0
22	4.8399196573336884E-4	0.0	0.005807903588800426	9.679839314667377E-4	0.0
23	4.8399196573336884E-4	0.0	0.005807903588800426	0.001693971880066791	0.0
24	4.8399196573336884E-4	0.0	0.005807903588800426	0.001693971880066791	0.0
25	4.8399196573336884E-4	0.0	0.005807903588800426	0.0019359678629334754	0.0
26	4.8399196573336884E-4	0.0	0.005807903588800426	0.0021779638458001597	0.0
27	4.8399196573336884E-4	0.0	0.005807903588800426	0.004113931708733635	0.0
28	7.259879486000532E-4	0.0	0.005807903588800426	0.013793771023401012	0.0
29	7.259879486000532E-4	0.0	0.005807903588800426	0.030007501875468866	0.0
30	7.259879486000532E-4	0.0	0.005807903588800426	0.054691092127870676	0.0
31	7.259879486000532E-4	0.0	0.005807903588800426	0.10139631682114077	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATG	20	0.001840572	37.0	2
GGTATCA	335	0.0	29.820896	1
TGCGGTA	40	5.9310645E-5	27.750002	36
TCGGAGC	40	5.9310645E-5	27.750002	3
CGAATGT	40	0.0019291055	23.125002	15
GTATCAA	895	0.0	21.70391	1
GCCGCTC	60	3.718856E-5	21.583332	27
GTCGCCC	140	0.0	21.142859	37
GCACCGC	175	0.0	21.142859	10
GCACCCT	45	0.0038217239	20.555555	6
TATACGG	45	0.0038217239	20.555555	2
AGGTCGC	145	0.0	20.413794	35
AACTTTG	210	0.0	20.261904	37
GTATTAT	55	5.135434E-4	20.181818	1
GTATTAG	220	0.0	20.181818	1
AGCTTCG	55	5.135434E-4	20.181818	21
CGCTCTC	55	5.135434E-4	20.181818	29
CCACATA	55	5.135434E-4	20.181818	13
AGGCACC	190	0.0	19.473684	8
TATTAGA	190	0.0	19.473684	2
>>END_MODULE