FastQCFastQC Report
Fri 10 Feb 2017
ERR1633222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648205
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43060.664296017463611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.4879629129673483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15980.2465269474934627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.12310920156431993No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7850.12110366319297136No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6880.1061392614990628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCC250.005494596429.630
AACAACG400.00193028423.1252
TCGCTCA400.00193028423.1259
GTATCAA17750.022.3042261
GGTATCA7450.022.100671
CCGTAAA450.003824035220.55555526
CGCCGTA450.003824035220.55555524
CCACGGA450.003824035220.5555553
TGTCGAC450.003824035220.55555536
TAAGGAC656.8961956E-519.9230773
TATACTG1355.638867E-1119.1851865
ACGCCGT500.00703165218.523
CGAGTCG500.00703165218.521
CACGCCG500.00703165218.522
CCGTCCG500.00703165218.528
ATACCAT801.6148992E-518.56
TGCGTTA500.00703165218.537
ACGGTAC1156.392111E-817.6956523
GCTTCGC1502.5102054E-1017.26666622
TCCACGG650.00157892917.0769232
Produced by FastQC (version 0.11.5)