FastQCFastQC Report
Fri 10 Feb 2017
ERR1633221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286318
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54381.899286806976858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45471.5880943566244525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24290.8483574207699132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.364978799796031No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9210.3216703106336311No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6850.23924447642132174No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6710.23435480829008307No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6450.22527399604635404No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.22492473403697985No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6080.21235130169950894No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5970.20850941959639283No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5190.18126698286520582No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5070.17707583875271551No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4860.1697413365558575No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4400.15367528412464462No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3980.13900627973092855No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3840.13411661159968985No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3820.13341808758094148No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3810.1330688255715673No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3560.12433727533721246No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3530.1232894893090899No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3530.1232894893090899No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG3520.12294022729971571No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3440.12014613122472216No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3400.11874908318722538No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3240.11316089103723832No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3220.11246236701848993No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3220.11246236701848993No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3210.11211310500911574No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3130.1093190089341222No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.10792196089662544No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3090.10792196089662544No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3050.10652491285912866No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2990.1044293408028835No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2990.1044293408028835No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2960.10338155477476092No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2920.10198450673726417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC200.001839628937.09
GGAATGT250.005489538429.59999828
GGCTTAA250.005489538429.5999981
GTTTAGG405.9247992E-527.751
TTTAGGA358.8530674E-426.4285722
GTATTAT358.8530674E-426.4285721
ATAATAT706.548362E-926.4285723
TTGTTAG358.8530674E-426.42857218
TCGACTA358.8530674E-426.42857219
TGTGCAA451.3198244E-424.66666610
TAGGACC601.3315548E-624.6666664
GTCCGGC1300.024.19230815
TGTCCGG1300.024.19230814
GGCAGCT1700.023.94117718
CCGCTCT1550.023.87096828
CGCTCTC1550.023.87096829
ACTAGTG551.8961966E-523.5454548
TAGGGGT551.8961966E-523.5454544
GCAGCTT1650.023.54545419
GGCGAGT1103.6379788E-1223.54545419
Produced by FastQC (version 0.11.5)