FastQCFastQC Report
Fri 10 Feb 2017
ERR1633220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556899
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36190.6498485362695929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.451787487497733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13430.2411568345427088No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11330.20344802199321602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.13251954124536047No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA6090.10935555639352916No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC5870.10540510936453468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGA451.3220156E-424.66666610
CTTCGCA400.001929944123.1259
TGCGTTA400.001929944123.12537
GTATCAA14550.022.6323031
TTTGCCG502.6996783E-422.29
GGTATCA5200.022.0576921
CCGGCTT450.003823368320.55555525
CGCTCTC450.003823368320.55555529
GCGGCTT450.003823368320.55555536
ATGCGTT450.003823368320.55555536
TGCGTTT450.003823368320.55555524
TAGTACC909.456198E-820.5555554
TAGTACA656.893968E-519.9230774
GCTTTAT2450.019.6326521
CTCGTCT1002.8683098E-718.513
CTATCGG1002.8683098E-718.512
ATCGGTC1002.8683098E-718.514
ATAGTAC1205.156835E-918.53
TGCCGAG609.228666E-418.511
TAGGACC1156.387381E-817.6956524
Produced by FastQC (version 0.11.5)