##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556899
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.874645133139044	33.0	33.0	33.0	33.0	33.0
2	31.9909444980149	33.0	33.0	33.0	33.0	33.0
3	31.71193519830346	33.0	33.0	33.0	27.0	33.0
4	32.00152451342164	33.0	33.0	33.0	33.0	33.0
5	32.091257122027514	33.0	33.0	33.0	33.0	33.0
6	35.26244076574029	37.0	37.0	37.0	33.0	37.0
7	35.39736828401559	37.0	37.0	37.0	33.0	37.0
8	35.546411467788595	37.0	37.0	37.0	33.0	37.0
9	35.646555299973606	37.0	37.0	37.0	33.0	37.0
10	35.656961136579525	37.0	37.0	37.0	33.0	37.0
11	35.67186150450979	37.0	37.0	37.0	33.0	37.0
12	35.65789487860456	37.0	37.0	37.0	33.0	37.0
13	35.67389418907199	37.0	37.0	37.0	33.0	37.0
14	35.644323297402224	37.0	37.0	37.0	33.0	37.0
15	35.66547075861153	37.0	37.0	37.0	33.0	37.0
16	35.640033471060285	37.0	37.0	37.0	33.0	37.0
17	35.645558709927656	37.0	37.0	37.0	33.0	37.0
18	35.633057340738624	37.0	37.0	37.0	33.0	37.0
19	35.627763741719775	37.0	37.0	37.0	33.0	37.0
20	35.6210533687437	37.0	37.0	37.0	33.0	37.0
21	35.614984045580975	37.0	37.0	37.0	33.0	37.0
22	35.52798622371382	37.0	37.0	37.0	33.0	37.0
23	35.57606136839894	37.0	37.0	37.0	33.0	37.0
24	35.56968498776259	37.0	37.0	37.0	33.0	37.0
25	35.59555323317154	37.0	37.0	37.0	33.0	37.0
26	35.48461570230868	37.0	37.0	37.0	33.0	37.0
27	35.48388307395057	37.0	37.0	37.0	33.0	37.0
28	35.52316667833844	37.0	37.0	37.0	33.0	37.0
29	35.523409092133406	37.0	37.0	37.0	33.0	37.0
30	35.517190729378214	37.0	37.0	37.0	33.0	37.0
31	35.51276084173252	37.0	37.0	37.0	33.0	37.0
32	35.49585113279068	37.0	37.0	37.0	33.0	37.0
33	35.492841610417685	37.0	37.0	37.0	33.0	37.0
34	35.470650872061185	37.0	37.0	37.0	33.0	37.0
35	35.39613646280564	37.0	37.0	37.0	33.0	37.0
36	35.41885153322236	37.0	37.0	37.0	33.0	37.0
37	35.425897694195896	37.0	37.0	37.0	33.0	37.0
38	35.41344839908134	37.0	37.0	37.0	33.0	37.0
39	35.300383013796036	37.0	37.0	37.0	33.0	37.0
40	35.079621259869384	37.0	37.0	37.0	33.0	37.0
41	35.247130987845196	37.0	37.0	37.0	33.0	37.0
42	35.2799574069984	37.0	37.0	37.0	33.0	37.0
43	34.947773294618955	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	18.0
16	29.0
17	29.0
18	11.0
19	17.0
20	43.0
21	89.0
22	256.0
23	587.0
24	1148.0
25	2107.0
26	3428.0
27	5350.0
28	7612.0
29	10111.0
30	13106.0
31	17374.0
32	22365.0
33	29191.0
34	42337.0
35	85792.0
36	315897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2230943133315	21.227727110301867	13.97973420674126	23.569444369625373
2	15.577330898421437	22.424712560087197	38.750294038955	23.247662502536368
3	17.6865104803564	27.728905959608475	30.774161921641085	23.810421638394036
4	12.02049204613404	17.954422615231845	40.97726876866361	29.047816569970497
5	13.353229221097543	37.34626925169555	35.80505621306557	13.495445314141344
6	28.436215543572533	40.932556890926364	17.426140107990857	13.205087457510247
7	25.64594298068411	32.78637598559164	24.048884986326065	17.518796047398183
8	24.796596869450298	34.697494518754745	21.54555853036188	18.960350081433077
9	26.049068143415592	15.280688239698762	20.931623148901327	37.73862046798432
10	14.640895386775698	28.59171950389568	34.256480977699724	22.510904131628894
11	32.80666691805875	24.516833393487865	24.71902445506277	17.957475233390614
12	22.872190469007844	26.29184106992471	30.676119009012403	20.159849452055038
13	27.955338400679473	22.60499659722858	26.754761635413242	22.684903366678697
14	21.619180497720414	21.654734520981364	29.075649264947508	27.650435716350717
15	23.186610139360997	29.590643904909147	25.274062262636495	21.94868369309336
16	21.91959403769804	29.121618103103074	26.13418232031302	22.824605538885866
17	21.85656645100817	28.080495745188983	27.173149888938568	22.889787914864275
18	22.30997003047231	27.421489354443086	29.18069524276395	21.087845372320654
19	24.204029815101123	26.546465337520807	28.91781094956177	20.3316938978163
20	24.11047604682357	26.56244669141083	28.30980123864471	21.01727602312089
21	22.471579227112993	27.089113106685414	28.70251158648157	21.736796079720023
22	21.98316032171004	27.389347080888992	28.90991005550378	21.717582541897183
23	22.112447679022587	26.95300224995915	29.06487531850479	21.869674752513472
24	21.84130336021433	27.405508000553063	28.57932946548656	22.173859173746045
25	22.370842827873634	27.660670965471297	28.488828315367776	21.47965789128729
26	22.503721500667087	27.490801743224537	28.528871482979856	21.47660527312852
27	22.33205662067987	27.26921757805275	28.385039297969648	22.013686503297723
28	22.14818126805758	27.21462958274301	28.749198687733323	21.887990461466085
29	22.63157233178727	27.425798933020168	28.346971353872068	21.59565738132049
30	22.8438190767087	26.951027026444653	28.40299587537417	21.802158021472476
31	22.14566734722095	27.549519751337314	28.647564459623737	21.657248441817995
32	21.432432092713398	27.35882089930131	28.818690642288818	22.390056365696474
33	21.237603227874356	27.393836225240126	29.44573432525467	21.922826221630853
34	22.528681143259373	26.767869936918544	28.743093451415785	21.960355468406302
35	21.945990206482684	27.490263045902395	28.45165820014042	22.1120885474745
36	22.18840400144371	27.414845420803413	28.727830360621944	21.668920217130935
37	22.294527373904423	26.846160614402255	28.762127423464577	22.097184588228746
38	22.592426993045418	27.060382582838184	28.334940447011036	22.012249977105363
39	22.042955724467095	27.2805302218176	28.55508808599046	22.121425967724846
40	21.87757564657146	27.458659469670444	28.834133298856706	21.829631584901392
41	21.74954524967723	26.699455377007318	28.891235215003082	22.659764158312367
42	21.656530178721813	27.380548357960777	28.817792813418592	22.145128649898815
43	22.149438228475898	27.03470467714972	28.720647729660136	22.095209364714247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	65.0
2	75.0
3	297.5
4	520.0
5	520.0
6	576.5
7	633.0
8	585.0
9	537.0
10	725.5
11	914.0
12	914.0
13	1477.0
14	2040.0
15	3517.5
16	4995.0
17	5972.5
18	6950.0
19	6950.0
20	6887.5
21	6825.0
22	7795.5
23	8766.0
24	10829.5
25	12893.0
26	12893.0
27	15077.5
28	17262.0
29	19347.5
30	21433.0
31	23814.0
32	26195.0
33	26195.0
34	28605.0
35	31015.0
36	32952.5
37	34890.0
38	35649.5
39	36409.0
40	36409.0
41	37112.0
42	37815.0
43	37643.0
44	37471.0
45	37333.0
46	37195.0
47	37195.0
48	36870.0
49	36545.0
50	36401.0
51	36257.0
52	35591.0
53	34925.0
54	34925.0
55	32888.5
56	30852.0
57	27591.0
58	24330.0
59	22581.0
60	20832.0
61	20832.0
62	18498.0
63	16164.0
64	13967.5
65	11771.0
66	9721.5
67	7672.0
68	7672.0
69	6469.0
70	5266.0
71	4581.5
72	3897.0
73	2742.5
74	1588.0
75	1588.0
76	1131.5
77	675.0
78	538.0
79	401.0
80	335.5
81	270.0
82	270.0
83	227.5
84	185.0
85	170.5
86	156.0
87	140.5
88	125.0
89	125.0
90	98.5
91	72.0
92	49.0
93	26.0
94	14.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	556899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.548764076295136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.31716037920131	56.7599515290248
2	6.204702512691128	7.886023522852083
3	1.42214867885664	2.711273726222263
4	0.690643472024569	1.755581562580907
5	0.42588508912224327	1.3532235526120682
6	0.29251263670932803	1.1153289923745668
7	0.22386862710181288	0.9958602197444039
8	0.16546533746310454	0.8412094154597911
9	0.12641879480396206	0.7230382349225896
>10	0.9519804322811101	12.200827192200087
>50	0.11623147192730292	5.049836691456299
>100	0.06097857702335086	6.6893914287963225
>500	8.588531975119742E-4	0.35185300180293183
>1k	0.0011451375966826324	1.5666009299509027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3619	0.6498485362695929	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2516	0.451787487497733	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1343	0.2411568345427088	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1133	0.20344802199321602	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.13251954124536047	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	609	0.10935555639352916	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	587	0.10540510936453468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.386973221356117E-4	0.0	0.0	0.0	0.0
3	5.386973221356117E-4	0.0	0.0	0.0	0.0
4	5.386973221356117E-4	0.0	0.0	0.0	0.0
5	5.386973221356117E-4	0.0	1.7956577404520387E-4	0.0	0.0
6	5.386973221356117E-4	0.0	3.5913154809040775E-4	0.0	0.0
7	5.386973221356117E-4	0.0	0.001436526192361631	0.0	0.0
8	5.386973221356117E-4	0.0	0.0017956577404520389	0.0	0.0
9	5.386973221356117E-4	0.0	0.002873052384723262	5.386973221356117E-4	0.0
10	7.182630961808155E-4	0.0	0.003950447028994486	7.182630961808155E-4	0.0
11	7.182630961808155E-4	0.0	0.004668710125175301	8.978288702260194E-4	0.0
12	7.182630961808155E-4	0.0	0.004848275899220505	8.978288702260194E-4	0.0
13	7.182630961808155E-4	0.0	0.005207407447310913	8.978288702260194E-4	0.0
14	7.182630961808155E-4	0.0	0.005925670543491728	8.978288702260194E-4	0.0
15	7.182630961808155E-4	0.0	0.0066439336396725435	8.978288702260194E-4	0.0
16	7.182630961808155E-4	0.0	0.007003065187762952	8.978288702260194E-4	0.0
17	7.182630961808155E-4	0.0	0.007003065187762952	8.978288702260194E-4	0.0
18	7.182630961808155E-4	0.0	0.007003065187762952	8.978288702260194E-4	0.0
19	7.182630961808155E-4	0.0	0.007003065187762952	0.0010773946442712234	0.0
20	7.182630961808155E-4	0.0	0.00736219673585336	0.001616091966406835	0.0
21	7.182630961808155E-4	0.0	0.00736219673585336	0.001975223514497243	0.0
22	7.182630961808155E-4	0.0	0.00736219673585336	0.0023343550625876505	0.0
23	7.182630961808155E-4	0.0	0.007541762509898563	0.00323218393281367	0.0
24	7.182630961808155E-4	0.0	0.007541762509898563	0.005207407447310913	0.0
25	7.182630961808155E-4	0.0	0.007541762509898563	0.005925670543491728	0.0
26	7.182630961808155E-4	0.0	0.007541762509898563	0.007003065187762952	0.0
27	7.182630961808155E-4	0.0	0.007541762509898563	0.008798722928214991	0.0
28	7.182630961808155E-4	0.0	0.007541762509898563	0.020111366693062835	0.0
29	7.182630961808155E-4	0.0	0.007541762509898563	0.044532311963210565	0.0
30	7.182630961808155E-4	0.0	0.007541762509898563	0.07882937480584451	0.0
31	7.182630961808155E-4	0.0	0.007541762509898563	0.13826564601480698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCCGA	45	1.3220156E-4	24.666666	10
CTTCGCA	40	0.0019299441	23.125	9
TGCGTTA	40	0.0019299441	23.125	37
GTATCAA	1455	0.0	22.632303	1
TTTGCCG	50	2.6996783E-4	22.2	9
GGTATCA	520	0.0	22.057692	1
CCGGCTT	45	0.0038233683	20.555555	25
CGCTCTC	45	0.0038233683	20.555555	29
GCGGCTT	45	0.0038233683	20.555555	36
ATGCGTT	45	0.0038233683	20.555555	36
TGCGTTT	45	0.0038233683	20.555555	24
TAGTACC	90	9.456198E-8	20.555555	4
TAGTACA	65	6.893968E-5	19.923077	4
GCTTTAT	245	0.0	19.632652	1
CTCGTCT	100	2.8683098E-7	18.5	13
CTATCGG	100	2.8683098E-7	18.5	12
ATCGGTC	100	2.8683098E-7	18.5	14
ATAGTAC	120	5.156835E-9	18.5	3
TGCCGAG	60	9.228666E-4	18.5	11
TAGGACC	115	6.387381E-8	17.695652	4
>>END_MODULE