FastQCFastQC Report
Fri 10 Feb 2017
ERR1633219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418271
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135583.2414391626481396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106932.556476542719911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56441.3493644072861852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.6854407788252115No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20960.5011105240382432No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG17740.4241269416239711No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17240.41217296919939467No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16750.40045807622330976No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14280.34140545244590226No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.33160319505774966No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12290.2938286421960882No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12020.2873734970868169No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11390.2723114918318506No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10860.25964028106179965No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10000.23907944849152823No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9910.23692773345510448No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.23525417731566378No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9530.22784271441242643No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.22545191992751112No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9350.22353928433957887No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9230.22067033095768054No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9160.21899677481823984No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8710.20823819963612109No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8570.20489108735723968No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8520.20369569011478203No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.20082673673288368No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8060.19269803548417175No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7890.18863368485981577No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7780.18600381092640897No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7740.18504749313244284No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7700.18409117533847674No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7630.18241761919903604No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7540.1802659041626123No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7230.17285444125937488No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7100.16974640842898503No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6990.16711653449557823No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6810.16281310442273073No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6800.1625740249742392No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6800.1625740249742392No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6700.1601832304893239No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6610.15803151545290015No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6510.15564072096798487No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6500.15540164151949334No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6300.1506200525496628No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6100.1458384635798322No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6060.1448821457858661No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6040.14440398688888306No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5890.14081779516151013No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5810.1389051595735779No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5770.13794884177961178No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5760.13770976233112026No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5670.1355580472946965No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5660.13531896784620495No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5450.13029829942788287No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5450.13029829942788287No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5440.13005921997939135No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5420.1295810610824083No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5300.12671210770050995No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5280.1262339488035269No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5270.12599486935503537No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5260.12575578990654385No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5150.12312591597313702No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5130.12264775707615397No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5110.12216959817917092No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5070.12121328038520482No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5040.12049604203973022No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4950.11834432700330647No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.11762708865783189No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4870.11643169141537424No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4850.11595353251839119No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4780.11427997637895049No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA4720.11284549968800132No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4660.11141102299705216No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4640.1109328641000691No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4530.10830299016666228No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4530.10830299016666228No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4520.10806391071817076No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4490.10734667237269617No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4440.10615127513023852No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG4400.10519495733627242No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC4400.10519495733627242No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4400.10519495733627242No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4340.10376048064532324No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4330.10352140119683172No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4290.10256508340286562No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4270.10208692450588254No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4260.10184784505739101No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10136968616040797No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4200.10041336836644184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTT303.5955996E-430.8333328
ATCTTGG303.5955996E-430.83333210
TGTCAAC358.8599266E-426.428572
ATAGATC358.8599266E-426.428576
TTATAGA358.8599266E-426.428574
TAGATCT358.8599266E-426.428577
ATGAACT358.8599266E-426.428574
GATCTTG358.8599266E-426.428579
TGGTGAC358.8599266E-426.4285714
CTAGTGG901.4188117E-1024.6666669
CGCTCTC4050.024.20987729
GCTCTCT4200.023.34523830
GACCGTT1600.023.1257
ATAGTTA400.00192914523.12514
GTGACAT400.00192914523.12516
GTTATAG400.00192914523.12511
GCCGCTC4100.023.01219427
ACTAGTG1450.022.9655178
CCGCTCT4350.022.5402328
ATACTAG2250.022.26
Produced by FastQC (version 0.11.5)