FastQCFastQC Report
Fri 10 Feb 2017
ERR1633218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845944
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30840.3645631389311822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.27034886470026387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11630.13747954947372404No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9400.1111184664705938No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8640.10213442024531175No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8510.10059767549625033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG451.3228088E-424.66666621
TTTCGCC601.3367098E-624.66666629
ATTTCGC601.3367098E-624.66666628
GGTATCA5350.023.8598121
GTATCAA14200.022.0176051
TTCGCCC705.099295E-621.14285930
CGCTTGA500.007033385318.49999818
TAATACT3550.018.2394374
AGGTCGC2200.017.6590935
TATACTT2100.017.6190475
GTCGCCC2350.017.31914737
TGCACCG752.0664532E-417.2666665
GAGTCGC650.001579467417.07692322
CATTTCG904.443724E-516.44444527
ACTGGTC1251.6560807E-716.288
GCACCGT803.3812446E-416.18756
ATCCGTA700.002591734515.85714312
TACTAGG700.002591734515.8571436
TAACGGC1751.3096724E-1015.85714236
GCGTAGG1059.337189E-615.85714226
Produced by FastQC (version 0.11.5)