##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845944
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870398040532233	33.0	33.0	33.0	33.0	33.0
2	31.988800677113378	33.0	33.0	33.0	33.0	33.0
3	31.699090010686287	33.0	33.0	33.0	27.0	33.0
4	31.98367977076497	33.0	33.0	33.0	33.0	33.0
5	32.08870563536121	33.0	33.0	33.0	33.0	33.0
6	35.25517291924761	37.0	37.0	37.0	33.0	37.0
7	35.39073153778501	37.0	37.0	37.0	33.0	37.0
8	35.52838485762651	37.0	37.0	37.0	33.0	37.0
9	35.6420271318196	37.0	37.0	37.0	33.0	37.0
10	35.658644071002335	37.0	37.0	37.0	33.0	37.0
11	35.66917550097879	37.0	37.0	37.0	33.0	37.0
12	35.65639096677794	37.0	37.0	37.0	33.0	37.0
13	35.66253794577419	37.0	37.0	37.0	33.0	37.0
14	35.63694759936828	37.0	37.0	37.0	33.0	37.0
15	35.65411540243799	37.0	37.0	37.0	33.0	37.0
16	35.641640581409646	37.0	37.0	37.0	33.0	37.0
17	35.63932127894991	37.0	37.0	37.0	33.0	37.0
18	35.61940270277938	37.0	37.0	37.0	33.0	37.0
19	35.614078473279555	37.0	37.0	37.0	33.0	37.0
20	35.62066992614168	37.0	37.0	37.0	33.0	37.0
21	35.61843573569882	37.0	37.0	37.0	33.0	37.0
22	35.52160426694911	37.0	37.0	37.0	33.0	37.0
23	35.57625682078246	37.0	37.0	37.0	33.0	37.0
24	35.565020852444135	37.0	37.0	37.0	33.0	37.0
25	35.577288803987024	37.0	37.0	37.0	33.0	37.0
26	35.47277833993739	37.0	37.0	37.0	33.0	37.0
27	35.48868601231287	37.0	37.0	37.0	33.0	37.0
28	35.50940014941887	37.0	37.0	37.0	33.0	37.0
29	35.51772694173609	37.0	37.0	37.0	33.0	37.0
30	35.516557833615465	37.0	37.0	37.0	33.0	37.0
31	35.51863953169477	37.0	37.0	37.0	33.0	37.0
32	35.49989360997891	37.0	37.0	37.0	33.0	37.0
33	35.50265502208184	37.0	37.0	37.0	33.0	37.0
34	35.47854113274638	37.0	37.0	37.0	33.0	37.0
35	35.428140633422544	37.0	37.0	37.0	33.0	37.0
36	35.455443859167985	37.0	37.0	37.0	33.0	37.0
37	35.44460271601903	37.0	37.0	37.0	33.0	37.0
38	35.44228341355929	37.0	37.0	37.0	33.0	37.0
39	35.35153154345914	37.0	37.0	37.0	33.0	37.0
40	35.1485866676754	37.0	37.0	37.0	33.0	37.0
41	35.313653149617465	37.0	37.0	37.0	33.0	37.0
42	35.35342765005721	37.0	37.0	37.0	33.0	37.0
43	35.03278349394286	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	34.0
16	53.0
17	45.0
18	24.0
19	25.0
20	45.0
21	126.0
22	370.0
23	819.0
24	1849.0
25	3217.0
26	5427.0
27	8161.0
28	11348.0
29	15339.0
30	20148.0
31	26060.0
32	33083.0
33	44317.0
34	63256.0
35	130064.0
36	482130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74335889846136	21.131067777536103	13.737670578667146	24.38790274533539
2	15.800691298714808	21.8509735869041	38.828693152265394	23.519641962115696
3	17.401270060429532	27.441650983989486	30.329549000879492	24.827529954701493
4	12.34337024673028	18.2032143971705	40.118967685804265	29.33444767029496
5	13.412117114135214	37.43084648629223	35.964791995687655	13.192244403884889
6	28.93394834646265	40.49972575016786	17.637810540650445	12.928515362719045
7	26.072884257113948	32.55617393113492	24.161883056088822	17.209058755662312
8	24.21909724520772	36.02354292955562	21.022431744890916	18.734928080345746
9	25.72250645432795	15.501144283782379	20.727258541936582	38.04909071995309
10	14.580870601363682	28.31712264641631	34.488689558646904	22.613317193573096
11	33.392872341431584	23.887515012814088	24.7424179378304	17.97719470792393
12	22.415195332078717	26.168635276093926	30.863745117880143	20.55242427394721
13	27.465174999763576	22.60338745827147	26.554239996973795	23.37719754499116
14	21.64398589031898	21.242895510813955	28.21286042574922	28.900258173117845
15	23.51018507135224	29.135143697455153	24.76334130864454	22.591329922548063
16	22.6349498311945	28.85711110900958	25.541406996207787	22.966532063588133
17	22.039402135365936	28.032588445570866	26.91608427981048	23.011925139252718
18	22.53659816725457	27.824891482178487	28.065569352108415	21.572940998458527
19	24.059275791305335	27.062666086644033	27.726066973700387	21.15199114835025
20	23.51834163963572	26.909228034006976	27.836712595632807	21.735717730724495
21	22.54700074709437	27.50524857437372	27.96674484363031	21.9810058349016
22	22.459169874128783	27.147187047842408	27.793920164928178	22.59972291310063
23	22.340840528451057	27.217877306299236	28.274212004577137	22.167070160672576
24	22.535652478178225	27.73067720794757	26.938307973104603	22.7953623407696
25	22.645588833303385	27.3954304303831	27.97419214510653	21.984788591206982
26	22.660010591717654	27.59260660280113	27.728431196391252	22.018951609089964
27	22.50787286156058	27.507494585930036	27.31020020237746	22.674432350131923
28	22.269559214321514	27.3026346897667	28.07857257690816	22.349233519003622
29	23.46632874043672	27.20960252688121	27.22142364033553	22.10264509234654
30	23.050816602517425	26.971407090776694	27.74923635607085	22.228539950635028
31	22.83307169268888	27.128036844046417	27.670980585003264	22.367910878261448
32	22.017533075475445	26.966915067664054	28.103396915162232	22.91215494169827
33	21.97320390002175	27.088317902839904	28.39431451727301	22.544163679865335
34	22.668167160001136	26.839010620088327	28.137559933045214	22.355262286865322
35	22.414604276406003	27.582676867499504	27.67168985181052	22.331029004283973
36	23.357574496656987	27.15487077158772	27.332778529075206	22.154776202680083
37	22.729282316559964	26.745269190395582	27.549341327558324	22.97610716548613
38	22.70800431234219	27.29483275488685	27.47758716889061	22.51957576388035
39	22.23705115232214	27.204164814692227	27.859527344599645	22.699256688385994
40	22.454796062150688	27.630670588124033	27.994051615709786	21.92048173401549
41	22.373112168181343	26.863480324938767	27.85172540971979	22.911682097160096
42	21.94672460588408	27.735169231060215	27.758693246834305	22.559412916221405
43	22.799617941613157	26.627767322659658	27.70798067011528	22.86463406561191
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	35.5
2	51.0
3	179.5
4	308.0
5	308.0
6	385.5
7	463.0
8	455.0
9	447.0
10	606.0
11	765.0
12	765.0
13	1247.0
14	1729.0
15	3072.0
16	4415.0
17	5195.5
18	5976.0
19	5976.0
20	5941.0
21	5906.0
22	7027.5
23	8149.0
24	10597.5
25	13046.0
26	13046.0
27	16520.0
28	19994.0
29	23732.0
30	27470.0
31	31542.0
32	35614.0
33	35614.0
34	42090.0
35	48566.0
36	52569.5
37	56573.0
38	59908.5
39	63244.0
40	63244.0
41	65801.0
42	68358.0
43	69394.0
44	70430.0
45	68547.5
46	66665.0
47	66665.0
48	63998.5
49	61332.0
50	60351.0
51	59370.0
52	55705.5
53	52041.0
54	52041.0
55	49606.5
56	47172.0
57	41121.0
58	35070.0
59	32285.0
60	29500.0
61	29500.0
62	25869.5
63	22239.0
64	18469.0
65	14699.0
66	12373.5
67	10048.0
68	10048.0
69	8230.5
70	6413.0
71	5324.5
72	4236.0
73	3329.0
74	2422.0
75	2422.0
76	1897.0
77	1372.0
78	1058.0
79	744.0
80	620.0
81	496.0
82	496.0
83	398.0
84	300.0
85	236.0
86	172.0
87	130.0
88	88.0
89	88.0
90	61.0
91	34.0
92	20.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	845944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.202125048573066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.65465863950233	53.901039127357365
2	7.895957847574167	9.822907148261404
3	2.064964600647121	3.853355589309869
4	0.9551654176168937	2.3765327499471414
5	0.5221475739947087	1.6239344345713966
6	0.34249449698052187	1.2782331317778333
7	0.23386919823844074	1.018301277968591
8	0.17157750400505567	0.8537988287715615
9	0.14290795814972043	0.800026081493864
>10	0.8474587824540306	10.179589438349296
>50	0.0961836053940125	4.197721807117985
>100	0.06782450899886755	7.661821746063207
>500	0.00421508247091213	1.6540429907816874
>1k	5.747839733061996E-4	0.7786956482288021
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3084	0.3645631389311822	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2287	0.27034886470026387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1163	0.13747954947372404	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	940	0.1111184664705938	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	864	0.10213442024531175	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	851	0.10059767549625033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	1.1821113454318489E-4	0.0	1.1821113454318489E-4	1.1821113454318489E-4	0.0
4	1.1821113454318489E-4	0.0	1.1821113454318489E-4	2.3642226908636977E-4	0.0
5	1.1821113454318489E-4	0.0	2.3642226908636977E-4	2.3642226908636977E-4	0.0
6	1.1821113454318489E-4	0.0	0.001300322479975034	2.3642226908636977E-4	0.0
7	1.1821113454318489E-4	0.0	0.002955278363579622	3.5463340362955467E-4	0.0
8	1.1821113454318489E-4	0.0	0.0036645451708387316	3.5463340362955467E-4	0.0
9	1.1821113454318489E-4	0.0	0.0039009674399251015	8.274779418022942E-4	0.0
10	2.3642226908636977E-4	0.0	0.004373811978097841	9.456890763454791E-4	0.0
11	2.3642226908636977E-4	0.0	0.006501612399875169	9.456890763454791E-4	0.0
12	2.3642226908636977E-4	0.0	0.007210879207134278	9.456890763454791E-4	0.0
13	2.3642226908636977E-4	0.0	0.007801934879850203	9.456890763454791E-4	0.0
14	2.3642226908636977E-4	0.0	0.008511201687109312	9.456890763454791E-4	0.0
15	2.3642226908636977E-4	0.0	0.009575101897997975	9.456890763454791E-4	0.0
16	2.3642226908636977E-4	0.0	0.010402579839800271	9.456890763454791E-4	0.0
17	2.3642226908636977E-4	0.0	0.010520790974343455	0.001063900210888664	0.0
18	2.3642226908636977E-4	0.0	0.01087542437797301	0.001300322479975034	0.0
19	3.5463340362955467E-4	0.0	0.01111184664705938	0.0015367447490614037	0.0
20	3.5463340362955467E-4	0.0	0.01111184664705938	0.0017731670181477734	0.0
21	3.5463340362955467E-4	0.0	0.01134826891614575	0.0017731670181477734	0.0
22	3.5463340362955467E-4	0.0	0.01134826891614575	0.002009589287234143	0.0
23	3.5463340362955467E-4	0.0	0.011466480050688935	0.0027188560944932526	0.0
24	3.5463340362955467E-4	0.0	0.011466480050688935	0.0028370672290364374	0.0
25	3.5463340362955467E-4	0.0	0.011466480050688935	0.0028370672290364374	0.0
26	3.5463340362955467E-4	0.0	0.011584691185232119	0.003191700632665992	0.0
27	3.5463340362955467E-4	0.0	0.011584691185232119	0.004610234247184211	0.0
28	3.5463340362955467E-4	0.0	0.011584691185232119	0.016549558836045884	0.0
29	3.5463340362955467E-4	0.0	0.011584691185232119	0.035108706959325914	0.0
30	3.5463340362955467E-4	0.0	0.011584691185232119	0.06312474584606073	0.0
31	3.5463340362955467E-4	0.0	0.011584691185232119	0.12270315765582591	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCG	45	1.3228088E-4	24.666666	21
TTTCGCC	60	1.3367098E-6	24.666666	29
ATTTCGC	60	1.3367098E-6	24.666666	28
GGTATCA	535	0.0	23.859812	1
GTATCAA	1420	0.0	22.017605	1
TTCGCCC	70	5.099295E-6	21.142859	30
CGCTTGA	50	0.0070333853	18.499998	18
TAATACT	355	0.0	18.239437	4
AGGTCGC	220	0.0	17.65909	35
TATACTT	210	0.0	17.619047	5
GTCGCCC	235	0.0	17.319147	37
TGCACCG	75	2.0664532E-4	17.266666	5
GAGTCGC	65	0.0015794674	17.076923	22
CATTTCG	90	4.443724E-5	16.444445	27
ACTGGTC	125	1.6560807E-7	16.28	8
GCACCGT	80	3.3812446E-4	16.1875	6
ATCCGTA	70	0.0025917345	15.857143	12
TACTAGG	70	0.0025917345	15.857143	6
TAACGGC	175	1.3096724E-10	15.857142	36
GCGTAGG	105	9.337189E-6	15.857142	26
>>END_MODULE