FastQCFastQC Report
Fri 10 Feb 2017
ERR1633217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514859
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139362.7067604917074384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106392.066390992485321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54601.06048452100478No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA34360.6673671820828615No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27720.5383998337408883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.5168405330391428No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT25570.496640827877147No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24710.47993722553165036No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22800.44283969008990814No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC22340.43390520511441No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19500.37874447178742143No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16060.31193006240543525No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15350.29813987907368816No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA14650.28454392367619097No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14510.2818247325966915No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14440.2804651370569418No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.2777459459774424No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC13930.2705595124101939No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13630.2647326743826951No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13230.2569635570126967No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA13030.2530789983276975No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12520.24317337368094954No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12170.23637539598220092No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12110.23521002837670119No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12100.2350158004424512No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12060.2342388887054514No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11920.23151969762595198No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11810.2293831903492024No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10590.20568738237070733No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10420.20238550748845802No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10300.2000547722774585No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT10290.19986054434320855No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10240.1988894046719587No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10200.1981124929349589No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10170.197529809132209No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10080.1957817577239594No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9840.19112028730196035No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9580.1860703610114614No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9570.18587613307721143No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9540.18529344927446156No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9510.18471076547171167No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9330.18121466265521238No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9180.178301243641463No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9160.1779127877729631No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9070.17616473636471344No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8920.17325131735096405No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8880.1724744056139642No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8750.16994944246871474No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8710.1691725307317149No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8690.16878407486321498No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8640.1678129351919652No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8580.1666475675864654No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8460.1643168323754659No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.16276300890146622No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8340.16198609716446638No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8310.1614034133617165No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8260.1604322736904667No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8220.15965536195346686No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8170.15868422228221707No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG7990.15518811946571778No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7940.154216979794468No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG7930.15402275186021805No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7870.15285738425471826No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7760.1507208769779687No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7710.14974973730671892No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7650.14858436970121916No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7620.1480016858984693No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7570.1470305462272195No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7540.1464478624244696No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.1460594065559697No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7450.14469981101621998No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7410.14392289927922014No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7390.14353444341072022No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7240.1406210243969708No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7210.14003834059422093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.13673646571197162No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6910.13421150256672215No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6860.13324036289547236No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6850.1330461349612224No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6610.12838466453922334No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC6550.1272192969337236No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6520.12663661313097374No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA6510.12644238519672377No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6490.12605392932822385No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6490.12605392932822385No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6470.12566547345972393No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6470.12566547345972393No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6360.12352896618297436No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6330.1229462823802245No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC6250.12139245890622481No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6230.1210040030377249No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6200.12042131923497501No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6140.11925595162947525No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA6130.11906172369522529No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG6100.11847903989247541No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6050.1175079002212256No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA6030.1171194443527257No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5980.1161483046814759No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5950.11556562087872602No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5790.11245797393072665No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5690.11051569458822706No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5670.11012723871972716No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5670.11012723871972716No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5500.10682536383747783No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5500.10682536383747783No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5420.10527154036347815No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA5410.1050773124292282No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5380.1044946286264783No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG5210.10119275374422901No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT5190.10080429787572909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC551.2732926E-1137.09
TAGTGCA554.984031E-1033.63636410
TATTATA451.0780059E-732.888892
GTTATAG401.5977967E-632.3753
GATTCAA303.5967433E-430.83333236
AGATCTT551.8515493E-830.2727288
ATAGATC551.8515493E-830.2727286
TGTCAAC250.005493560329.62
ATGCTGC250.005493560329.635
AGTGGGC250.005493560329.626
TCGATTC250.005493560329.634
AGGCCAG250.005493560329.624
GGGGTGG250.005493560329.617
TCTCAAC250.005493560329.62
ATTATAG502.715842E-729.63
GTGACAT502.715842E-729.616
CCGCCGG250.005493560329.635
ATAGTTA454.002608E-628.7777776
TTATAGT405.9338567E-527.7500024
TATAGTT405.9338567E-527.7500025
Produced by FastQC (version 0.11.5)