FastQCFastQC Report
Fri 10 Feb 2017
ERR1633216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620744
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56930.9171252561442398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.6624308893843517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22470.36198497287126413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11080.1784954828399469No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7830.1261389558336448No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7400.11921178456819558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.025.2369941
GCCGCTC1400.025.10714127
GGCCGTT451.3222545E-424.66666614
CCGCTCT1500.024.66666628
GTATCAA23350.023.0556761
GGTACCG502.700163E-422.1999996
CGGCCGT603.722732E-521.58333413
GCCGGCA1550.021.48387115
CGCTCTC1650.021.3030329
TTCGCCG1650.021.3030324
CCGGCAG1750.021.14285716
AGCTTCG1750.021.14285721
CGAGTCG1151.3460522E-1020.91304421
GCTTCGC1800.020.55555522
CTCGGTA450.003823855720.55555527
TCGGTAG450.003823855720.55555528
CGCCGTC1101.7498678E-920.18181826
GAGTCGC1202.382876E-1020.04166822
AGTCGCC1254.110916E-1019.2423
TCGCCGT1205.160473E-918.525
Produced by FastQC (version 0.11.5)