##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620744
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900193960795434	33.0	33.0	33.0	33.0	33.0
2	32.02392612735685	33.0	33.0	33.0	33.0	33.0
3	31.73150445272125	33.0	33.0	33.0	27.0	33.0
4	32.01493691441238	33.0	33.0	33.0	33.0	33.0
5	32.115965679893804	33.0	33.0	33.0	33.0	33.0
6	35.28300555462477	37.0	37.0	37.0	33.0	37.0
7	35.42834888456433	37.0	37.0	37.0	33.0	37.0
8	35.56394423466034	37.0	37.0	37.0	33.0	37.0
9	35.66714297681492	37.0	37.0	37.0	33.0	37.0
10	35.67446161380537	37.0	37.0	37.0	33.0	37.0
11	35.69736799711314	37.0	37.0	37.0	33.0	37.0
12	35.68015317103347	37.0	37.0	37.0	33.0	37.0
13	35.696746162669314	37.0	37.0	37.0	33.0	37.0
14	35.678577642313094	37.0	37.0	37.0	33.0	37.0
15	35.7044208240434	37.0	37.0	37.0	33.0	37.0
16	35.67359652288222	37.0	37.0	37.0	33.0	37.0
17	35.67904160169088	37.0	37.0	37.0	33.0	37.0
18	35.65380575567384	37.0	37.0	37.0	33.0	37.0
19	35.661506192568915	37.0	37.0	37.0	33.0	37.0
20	35.65777357493588	37.0	37.0	37.0	33.0	37.0
21	35.65109932597013	37.0	37.0	37.0	33.0	37.0
22	35.55182812882605	37.0	37.0	37.0	33.0	37.0
23	35.61055282048638	37.0	37.0	37.0	33.0	37.0
24	35.60177303364994	37.0	37.0	37.0	33.0	37.0
25	35.63061423066514	37.0	37.0	37.0	33.0	37.0
26	35.51633040351578	37.0	37.0	37.0	33.0	37.0
27	35.53580542059206	37.0	37.0	37.0	33.0	37.0
28	35.54797952134857	37.0	37.0	37.0	33.0	37.0
29	35.55566384854304	37.0	37.0	37.0	33.0	37.0
30	35.55818662765971	37.0	37.0	37.0	33.0	37.0
31	35.539483265243	37.0	37.0	37.0	33.0	37.0
32	35.5202402278556	37.0	37.0	37.0	33.0	37.0
33	35.508794285567	37.0	37.0	37.0	33.0	37.0
34	35.48097444357094	37.0	37.0	37.0	33.0	37.0
35	35.4039378552189	37.0	37.0	37.0	33.0	37.0
36	35.42432307038006	37.0	37.0	37.0	33.0	37.0
37	35.412024924928794	37.0	37.0	37.0	33.0	37.0
38	35.388957444614846	37.0	37.0	37.0	33.0	37.0
39	35.27570947121519	37.0	37.0	37.0	33.0	37.0
40	35.06776384467671	37.0	37.0	37.0	33.0	37.0
41	35.2123902929388	37.0	37.0	37.0	33.0	37.0
42	35.233611601562	37.0	37.0	37.0	33.0	37.0
43	34.90229949866612	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	9.0
15	21.0
16	31.0
17	31.0
18	28.0
19	33.0
20	45.0
21	96.0
22	268.0
23	582.0
24	1253.0
25	2232.0
26	3707.0
27	5808.0
28	8288.0
29	11221.0
30	15025.0
31	19275.0
32	24494.0
33	31845.0
34	46705.0
35	94311.0
36	355436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92114623741832	20.7045094273968	14.12933512043612	23.24500921474875
2	15.612555256273117	22.55261428221618	39.18990759475726	22.644922866753443
3	17.83408297140206	27.924393953062776	30.949634631989998	23.291888443545165
4	11.873332645986107	18.075084092637223	41.04928924000876	29.00229402136791
5	13.320305955434126	37.36661167888856	35.37448610055031	13.938596265127009
6	27.74944260435864	41.228751304885755	17.556996120784092	13.464809969971517
7	25.22231386851907	33.21755828489683	23.86797133762066	17.69215650896344
8	24.713569523023985	34.76376735014757	21.164924671039913	19.357738455788535
9	26.27508280386117	15.125397909605248	20.814377585606948	37.78514170092663
10	14.746658848091968	28.395119405100978	33.94152823063936	22.916693516167697
11	32.915662495328185	24.5292745479618	24.594035544443443	17.96102741226657
12	22.732559638111685	26.448906473522094	30.91435438763806	19.90417950072816
13	28.480017527354274	22.781372030982176	26.568762646114983	22.169847795548566
14	21.763400048973487	21.769521735208073	29.42340159550475	27.04367662031369
15	23.50550307373088	29.11747838078177	25.827394223705745	21.549624321781604
16	21.39174925573183	29.11651179874473	26.470976763367833	23.020762182155607
17	21.638388772183056	27.646179423401595	27.37183122188857	23.343600582526776
18	22.668443029654735	26.439401750157877	29.470603018313508	21.42155220187388
19	24.273130308146353	26.440368332194915	29.348169293621844	19.938332066036885
20	24.253798667405565	25.948055881329502	28.94381580812702	20.854329643137913
21	22.64862809789543	26.885640457257743	29.152758625133714	21.31297281971312
22	22.124579536813886	26.891762143492326	29.40358666374544	21.580071655948345
23	22.050958204992718	26.643511656979367	29.553084685474207	21.752445452553708
24	21.760661399868546	26.888540203368862	29.361701442140397	21.989096954622195
25	22.165337079375718	27.083950871857	29.415668939208434	21.335043109558853
26	22.16791463147449	26.994219839418506	29.123116775997836	21.714748753109173
27	22.256195790857422	26.80799170028224	29.279541968992046	21.656270539868284
28	21.78482595079453	26.976015877720926	29.279541968992046	21.959616202492494
29	22.41278208085781	27.043032232288994	28.91932906318869	21.624856623664506
30	22.75221347286482	26.68555797559058	29.121666902942277	21.440561648602323
31	22.21382727823386	26.963933602257935	29.15936360238681	21.662875517121392
32	21.413819545577564	26.85905945123916	29.473019473406104	22.25410152977717
33	21.235485159743792	27.085884035931073	29.806329179178537	21.872301625146598
34	22.02018867681363	26.595988040158264	29.348169293621844	22.035653989406264
35	21.785470338819223	27.092489013184178	29.087836531645895	22.034204116350704
36	22.053696854097662	27.072674081424868	29.249577925843827	21.62405113863364
37	22.311613160981018	26.82539017694895	29.02839173636797	21.83460492570206
38	22.304041601690873	27.051087082597657	28.68299975513255	21.96187156057892
39	22.085432964313792	26.755474076269763	29.027102960318587	22.131989999097858
40	21.787081308880957	27.064297037103863	29.32642119778846	21.82220045622672
41	21.874073692214505	26.591799517997757	29.20865928627582	22.325467503511913
42	21.530292681040816	27.231032438493163	29.18884435451651	22.049830525949506
43	21.964288015671517	26.96264482620855	28.87808822960834	22.194978928511592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	73.5
2	100.0
3	361.0
4	622.0
5	622.0
6	756.0
7	890.0
8	853.0
9	816.0
10	1103.5
11	1391.0
12	1391.0
13	2257.5
14	3124.0
15	5347.0
16	7570.0
17	8685.5
18	9801.0
19	9801.0
20	9011.5
21	8222.0
22	8803.5
23	9385.0
24	11421.5
25	13458.0
26	13458.0
27	15796.5
28	18135.0
29	20528.5
30	22922.0
31	25286.5
32	27651.0
33	27651.0
34	30475.5
35	33300.0
36	34588.5
37	35877.0
38	37477.5
39	39078.0
40	39078.0
41	40061.5
42	41045.0
43	41325.0
44	41605.0
45	42014.0
46	42423.0
47	42423.0
48	42722.0
49	43021.0
50	43081.0
51	43141.0
52	41640.0
53	40139.0
54	40139.0
55	37570.0
56	35001.0
57	31369.0
58	27737.0
59	25769.0
60	23801.0
61	23801.0
62	20778.0
63	17755.0
64	14753.0
65	11751.0
66	9891.5
67	8032.0
68	8032.0
69	6817.0
70	5602.0
71	4635.5
72	3669.0
73	2717.0
74	1765.0
75	1765.0
76	1263.0
77	761.0
78	596.5
79	432.0
80	338.5
81	245.0
82	245.0
83	208.0
84	171.0
85	150.0
86	129.0
87	103.5
88	78.0
89	78.0
90	58.0
91	38.0
92	24.5
93	11.0
94	7.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620744.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.82887172775499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98133966300817	58.575411948399626
2	6.8067818083794664	8.961655330852562
3	1.4712393658008405	2.9055008247638137
4	0.6911339955702183	1.8198628456433072
5	0.38263197051473813	1.259411545297641
6	0.2693292655374271	1.0637785004156248
7	0.18916635422803954	0.8716825367379274
8	0.14867784519830154	0.7829835840254404
9	0.11979751868810515	0.7097521941920344
>10	0.7995797119926015	10.420434572287366
>50	0.08547817063701167	3.814766206209329
>100	0.05212681857619088	5.966331442776573
>500	0.0017293257347528847	0.7082447033900708
>1k	7.411396006083791E-4	1.214342870313125
>5k	2.4704653353612636E-4	0.9258408946956769
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5693	0.9171252561442398	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4112	0.6624308893843517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2247	0.36198497287126413	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1108	0.1784954828399469	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	783	0.1261389558336448	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	740	0.11921178456819558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.610970061732373E-4	0.0	0.0	0.0	0.0
3	1.610970061732373E-4	0.0	3.221940123464746E-4	0.0	0.0
4	1.610970061732373E-4	0.0	6.443880246929492E-4	0.0	0.0
5	1.610970061732373E-4	0.0	0.0016109700617323727	0.0	0.0
6	1.610970061732373E-4	0.0	0.0027386491049450337	0.0	0.0
7	1.610970061732373E-4	0.0	0.007410462283968915	0.0	0.0
8	1.610970061732373E-4	0.0	0.008699238333354812	0.0	0.0
9	1.610970061732373E-4	0.0	0.009665820370394236	9.665820370394236E-4	0.0
10	8.054850308661863E-4	0.0	0.011437887438299847	0.001127679043212661	0.0
11	9.665820370394236E-4	0.0	0.016109700617323728	0.001127679043212661	0.0
12	9.665820370394236E-4	0.0	0.01788176768522934	0.001127679043212661	0.0
13	9.665820370394236E-4	0.0	0.01965383475313495	0.001127679043212661	0.0
14	9.665820370394236E-4	0.0	0.02013712577165466	0.001127679043212661	0.0
15	9.665820370394236E-4	0.0	0.02142590182104056	0.001127679043212661	0.0
16	9.665820370394236E-4	0.0	0.022875774876599694	0.001127679043212661	0.0
17	0.001127679043212661	0.0	0.02303687188277293	0.0016109700617323727	0.0
18	0.001127679043212661	0.0	0.023520162901292642	0.0020942610802520845	0.0
19	0.001127679043212661	0.0	0.024003453919812356	0.0020942610802520845	0.0
20	0.0012887760493858983	0.0	0.024647841944505304	0.002255358086425322	0.0
21	0.0012887760493858983	0.0	0.025131132963025014	0.0025775520987717966	0.0
22	0.0012887760493858983	0.0	0.02609771500006444	0.004188522160504169	0.0
23	0.0012887760493858983	0.0	0.026258812006237676	0.004994007191370355	0.0
24	0.0012887760493858983	0.0	0.026258812006237676	0.006443880246929491	0.0
25	0.0012887760493858983	0.0	0.026258812006237676	0.007410462283968915	0.0
26	0.0012887760493858983	0.0	0.026258812006237676	0.008054850308661864	0.0
27	0.0012887760493858983	0.0	0.026258812006237676	0.009665820370394236	0.0
28	0.0012887760493858983	0.0	0.026258812006237676	0.022070289845733507	0.0
29	0.0012887760493858983	0.0	0.026258812006237676	0.05267872101864859	0.0
30	0.0012887760493858983	0.0	0.026258812006237676	0.08779786836441432	0.0
31	0.0012887760493858983	0.0	0.026258812006237676	0.16448004330287527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	865	0.0	25.236994	1
GCCGCTC	140	0.0	25.107141	27
GGCCGTT	45	1.3222545E-4	24.666666	14
CCGCTCT	150	0.0	24.666666	28
GTATCAA	2335	0.0	23.055676	1
GGTACCG	50	2.700163E-4	22.199999	6
CGGCCGT	60	3.722732E-5	21.583334	13
GCCGGCA	155	0.0	21.483871	15
CGCTCTC	165	0.0	21.30303	29
TTCGCCG	165	0.0	21.30303	24
CCGGCAG	175	0.0	21.142857	16
AGCTTCG	175	0.0	21.142857	21
CGAGTCG	115	1.3460522E-10	20.913044	21
GCTTCGC	180	0.0	20.555555	22
CTCGGTA	45	0.0038238557	20.555555	27
TCGGTAG	45	0.0038238557	20.555555	28
CGCCGTC	110	1.7498678E-9	20.181818	26
GAGTCGC	120	2.382876E-10	20.041668	22
AGTCGCC	125	4.110916E-10	19.24	23
TCGCCGT	120	5.160473E-9	18.5	25
>>END_MODULE