FastQCFastQC Report
Fri 10 Feb 2017
ERR1633215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753799
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45560.6044051530978417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.4497220081215284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17510.2322900401831257No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11590.15375451546101812No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9930.1317327298125893No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9810.1301407935006547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.11647667348988258No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8730.11581336669324316No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8550.11342546222534124No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8490.11262949406937393No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8050.10679239425894702No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG8020.10639441018096336No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC7540.1000266649332249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG250.00549515829.69
GGTATCA6150.027.3739831
ATGCGTT659.382893E-825.61538536
TGCGTTA659.382893E-825.61538537
GTATCAA18500.024.81
CGAGTCG705.09808E-621.14285721
CGAATCA450.003824604220.55555533
TTAACGG450.003824604220.55555535
GTCGCCA1202.382876E-1020.04166612
TCGCCAT1303.274181E-1119.92307713
CCGTCCG759.258836E-619.73333228
CGCCGTC759.258836E-619.73333226
CGAACTA609.2327956E-418.524
ACGCAAT500.007032690618.511
TGCGGTA609.2327956E-418.536
GACCATA609.2327956E-418.511
CGGGATA609.2327956E-418.517
CCGGTTA500.007032690618.529
GCCGTCC801.6154672E-518.527
AATGCGT953.6030578E-617.52631435
Produced by FastQC (version 0.11.5)