##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753799
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91263055536025	33.0	33.0	33.0	33.0	33.0
2	32.02118203924388	33.0	33.0	33.0	33.0	33.0
3	31.734125410089426	33.0	33.0	33.0	27.0	33.0
4	32.01313347457346	33.0	33.0	33.0	33.0	33.0
5	32.125820013027344	33.0	33.0	33.0	33.0	33.0
6	35.30115720503742	37.0	37.0	37.0	33.0	37.0
7	35.441483737707266	37.0	37.0	37.0	33.0	37.0
8	35.57792992561678	37.0	37.0	37.0	33.0	37.0
9	35.67517070200412	37.0	37.0	37.0	33.0	37.0
10	35.70166980852986	37.0	37.0	37.0	33.0	37.0
11	35.70797254971153	37.0	37.0	37.0	33.0	37.0
12	35.70542412499884	37.0	37.0	37.0	33.0	37.0
13	35.70220576042154	37.0	37.0	37.0	33.0	37.0
14	35.67049969554218	37.0	37.0	37.0	33.0	37.0
15	35.705924258323506	37.0	37.0	37.0	33.0	37.0
16	35.6815490601606	37.0	37.0	37.0	33.0	37.0
17	35.68872869292743	37.0	37.0	37.0	33.0	37.0
18	35.66981914276883	37.0	37.0	37.0	33.0	37.0
19	35.673488555967836	37.0	37.0	37.0	33.0	37.0
20	35.67009109855545	37.0	37.0	37.0	33.0	37.0
21	35.65125451214448	37.0	37.0	37.0	33.0	37.0
22	35.56922866705846	37.0	37.0	37.0	33.0	37.0
23	35.610410732834616	37.0	37.0	37.0	33.0	37.0
24	35.59883204939248	37.0	37.0	37.0	33.0	37.0
25	35.62531258332792	37.0	37.0	37.0	33.0	37.0
26	35.52078073863191	37.0	37.0	37.0	33.0	37.0
27	35.536746533227024	37.0	37.0	37.0	33.0	37.0
28	35.559865428317096	37.0	37.0	37.0	33.0	37.0
29	35.56847249731029	37.0	37.0	37.0	33.0	37.0
30	35.56952052204898	37.0	37.0	37.0	33.0	37.0
31	35.5602793317582	37.0	37.0	37.0	33.0	37.0
32	35.539804377559534	37.0	37.0	37.0	33.0	37.0
33	35.54333582294484	37.0	37.0	37.0	33.0	37.0
34	35.5133225170105	37.0	37.0	37.0	33.0	37.0
35	35.4533794817982	37.0	37.0	37.0	33.0	37.0
36	35.473400734147965	37.0	37.0	37.0	33.0	37.0
37	35.47100884983928	37.0	37.0	37.0	33.0	37.0
38	35.45423514756586	37.0	37.0	37.0	33.0	37.0
39	35.36426686689688	37.0	37.0	37.0	33.0	37.0
40	35.147811286563126	37.0	37.0	37.0	33.0	37.0
41	35.308561035501505	37.0	37.0	37.0	33.0	37.0
42	35.34273725489155	37.0	37.0	37.0	33.0	37.0
43	35.01989787728559	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	34.0
16	42.0
17	27.0
18	29.0
19	21.0
20	45.0
21	114.0
22	338.0
23	750.0
24	1494.0
25	2746.0
26	4512.0
27	7007.0
28	9899.0
29	13395.0
30	17483.0
31	22430.0
32	28920.0
33	38582.0
34	55708.0
35	114528.0
36	435693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.558424725954794	21.544602738926425	14.576299517510636	23.320673017608144
2	15.02562354155418	23.034124481459912	40.10604949064671	21.834202486339198
3	17.30195980626135	28.002557711007842	32.03612634137217	22.65935614135864
4	12.174200284160632	18.46659387980085	40.705148189371435	28.65405764666708
5	12.903970421823324	38.251045703164905	35.7190709990329	13.125912875978877
6	27.4476352449393	41.110030658040145	18.316819205119668	13.125514891900892
7	24.225556149583642	33.78473571867301	24.44272279480339	17.546985336939954
8	23.57100500265986	36.4355750007628	21.616637857041464	18.376782139535873
9	25.173686884700032	15.86231873483515	21.145159385990166	37.818834994474656
10	13.865632615591158	29.243870050238858	34.98664763418365	21.903849699986335
11	31.95573355761947	24.906374245654344	25.3871390118586	17.750753184867584
12	21.52072369424741	26.76867440789919	31.799591137690552	19.911010760162856
13	27.22303956359719	23.247709269977808	27.678731332888475	21.850519833536527
14	21.279280020270654	22.42772940797215	29.29123015551891	27.00176041623828
15	22.84349010810574	29.739094904609846	26.007596189435116	21.409818797849294
16	21.55985879524913	30.01542851608983	26.108153499805653	22.316559188855383
17	21.34216150459207	28.544081379784263	27.51807842674241	22.595678688881254
18	21.695040720404247	27.838588270878578	29.217072455654623	21.249298553062555
19	23.404514996703366	27.341108173399007	29.618373067621473	19.63600376227615
20	23.540360228655118	26.767215132946582	29.405451585900224	20.28697305249808
21	21.667579819023373	28.29812721959037	28.519671689667938	21.514621271718323
22	21.259248155012145	28.01582384694063	28.976690072552497	21.748237925494728
23	21.24133887150288	27.894704025874272	29.243339404801542	21.620617697821302
24	22.13759901512207	27.83712899592597	28.885949702772223	21.139322286179738
25	22.09368810518454	27.373477545075016	29.26774909491788	21.265085254822573
26	21.83393716362054	27.519405040335688	28.803036353192297	21.843621442851475
27	21.760044786474907	27.670638989969476	28.859682753625304	21.70963346993031
28	21.141975513366297	28.009190778974236	29.054694951837295	21.794138755822175
29	21.88925695046027	28.173027557744174	28.50799749004708	21.429718001748476
30	21.544735400285752	28.367641771878176	28.75488027975627	21.332742548079793
31	21.894032759396072	27.828108023491673	28.941402150971278	21.336457066140973
32	21.103105735083226	27.855038279435234	29.1089534478024	21.93290253767914
33	21.33061996633055	27.65949543578593	29.543684722319874	21.466199875563646
34	21.201805786423172	27.745990642067714	28.840712179241418	22.2114913922677
35	21.62977133161493	28.115187205077213	28.7502371321798	21.50480433112806
36	21.816558525548587	27.98371979798328	28.517018462481374	21.682703213986755
37	22.215736555766192	27.341771480195646	28.46236198243829	21.98012998159987
38	22.09435141198118	27.9719129370031	28.551510415906627	21.382225235109097
39	21.66399796232152	27.673424878515362	28.83023193185451	21.832345227308604
40	21.71931774916125	27.587858301748874	28.920308994838145	21.772514954251733
41	21.484374481791566	27.80953543318577	29.112137320426267	21.5939527645964
42	21.77954600629611	27.54474335996731	28.79282142852405	21.88288920521253
43	21.39244015977734	27.640524861402042	28.857294849157398	22.109740129663212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	78.0
2	103.0
3	318.5
4	534.0
5	534.0
6	654.5
7	775.0
8	718.5
9	662.0
10	940.5
11	1219.0
12	1219.0
13	1900.5
14	2582.0
15	5023.0
16	7464.0
17	8680.0
18	9896.0
19	9896.0
20	9829.5
21	9763.0
22	11703.0
23	13643.0
24	16219.0
25	18795.0
26	18795.0
27	21867.0
28	24939.0
29	30015.5
30	35092.0
31	36553.0
32	38014.0
33	38014.0
34	40954.5
35	43895.0
36	45202.0
37	46509.0
38	48983.0
39	51457.0
40	51457.0
41	53733.0
42	56009.0
43	52772.0
44	49535.0
45	50391.0
46	51247.0
47	51247.0
48	50135.5
49	49024.0
50	49726.0
51	50428.0
52	50152.5
53	49877.0
54	49877.0
55	43782.0
56	37687.0
57	34046.5
58	30406.0
59	26873.5
60	23341.0
61	23341.0
62	20372.5
63	17404.0
64	14558.5
65	11713.0
66	9916.5
67	8120.0
68	8120.0
69	6666.0
70	5212.0
71	4402.0
72	3592.0
73	2868.5
74	2145.0
75	2145.0
76	1597.5
77	1050.0
78	839.0
79	628.0
80	497.5
81	367.0
82	367.0
83	307.5
84	248.0
85	209.0
86	170.0
87	144.5
88	119.0
89	119.0
90	88.5
91	58.0
92	40.5
93	23.0
94	12.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	753799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.10705239465229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.11953135846532	54.72844351072411
2	7.463139555329362	9.270271987828856
3	1.656487172843166	3.0863860690451985
4	0.6942279046345817	1.7246579538787858
5	0.41256775193078316	1.2811683492754529
6	0.2634537544108599	0.9817401677257878
7	0.19119675847103612	0.8312266967233825
8	0.15193022460935832	0.7548750736115767
9	0.10197693449993431	0.57001381326301
>10	0.7593436690955316	9.609520075727108
>50	0.09318055351168998	4.052487603745983
>100	0.08778769237797532	9.727215577905563
>500	0.004313891517025101	1.9277066071712496
>1k	8.627783034050202E-4	1.454286513373946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4556	0.6044051530978417	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3390	0.4497220081215284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.2322900401831257	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1159	0.15375451546101812	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	993	0.1317327298125893	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	981	0.1301407935006547	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	878	0.11647667348988258	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	873	0.11581336669324316	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	855	0.11342546222534124	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	849	0.11262949406937393	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	805	0.10679239425894702	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	802	0.10639441018096336	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	754	0.1000266649332249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.65322718655769E-4	0.0	0.0
2	0.0	0.0	2.65322718655769E-4	0.0	0.0
3	0.0	0.0	2.65322718655769E-4	0.0	0.0
4	0.0	0.0	5.30645437311538E-4	2.65322718655769E-4	0.0
5	0.0	0.0	6.633067966394224E-4	2.65322718655769E-4	0.0
6	0.0	0.0	0.0011939522339509605	2.65322718655769E-4	0.0
7	0.0	0.0	0.003979840779836535	3.979840779836535E-4	0.0
8	0.0	0.0	0.0046431475764759575	3.979840779836535E-4	0.0
9	0.0	0.0	0.00650040660706634	0.0013266135932788448	0.0
10	0.0	0.0	0.007163713403705763	0.001591936311934614	0.0
11	0.0	0.0	0.01034758602757499	0.001591936311934614	0.0
12	0.0	0.0	0.011674199620853835	0.001591936311934614	0.0
13	0.0	0.0	0.012072183698837488	0.001591936311934614	0.0
14	0.0	0.0	0.012602829136149027	0.002122581749246152	0.0
15	0.0	0.0	0.014327426807411526	0.002255243108574036	0.0
16	0.0	0.0	0.015256056322706716	0.002387904467901921	0.0
17	0.0	0.0	0.015521379041362485	0.0027858885458855744	0.0
18	0.0	0.0	0.016184685838001908	0.0027858885458855744	0.0
19	0.0	0.0	0.016184685838001908	0.0029185499052134588	0.0
20	0.0	0.0	0.016450008556657677	0.0029185499052134588	0.0
21	0.0	0.0	0.017245976712624983	0.0029185499052134588	0.0
22	0.0	0.0	0.01777662214993652	0.004112502139164419	0.0
23	0.0	0.0	0.017909283509264406	0.004908470295131726	0.0
24	0.0	0.0	0.018174606227920175	0.006102422529082686	0.0
25	0.0	0.0	0.018174606227920175	0.0066330679663942245	0.0
26	0.0	0.0	0.018174606227920175	0.0070310520443778775	0.0
27	0.0	0.0	0.018174606227920175	0.0076943588410173	0.0
28	0.0	0.0	0.018307267587248058	0.019633881180526905	0.0
29	0.0	0.0	0.018307267587248058	0.0419209895476115	0.0
30	0.0	0.0	0.018307267587248058	0.07163713403705763	0.0
31	0.0	0.0	0.018307267587248058	0.13491660243645853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	25	0.005495158	29.6	9
GGTATCA	615	0.0	27.373983	1
ATGCGTT	65	9.382893E-8	25.615385	36
TGCGTTA	65	9.382893E-8	25.615385	37
GTATCAA	1850	0.0	24.8	1
CGAGTCG	70	5.09808E-6	21.142857	21
CGAATCA	45	0.0038246042	20.555555	33
TTAACGG	45	0.0038246042	20.555555	35
GTCGCCA	120	2.382876E-10	20.041666	12
TCGCCAT	130	3.274181E-11	19.923077	13
CCGTCCG	75	9.258836E-6	19.733332	28
CGCCGTC	75	9.258836E-6	19.733332	26
CGAACTA	60	9.2327956E-4	18.5	24
ACGCAAT	50	0.0070326906	18.5	11
TGCGGTA	60	9.2327956E-4	18.5	36
GACCATA	60	9.2327956E-4	18.5	11
CGGGATA	60	9.2327956E-4	18.5	17
CCGGTTA	50	0.0070326906	18.5	29
GCCGTCC	80	1.6154672E-5	18.5	27
AATGCGT	95	3.6030578E-6	17.526314	35
>>END_MODULE