FastQCFastQC Report
Fri 10 Feb 2017
ERR1633214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445515
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106072.380840151285591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80641.810040065991044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40050.8989596309888557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.46238622717529154No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG16280.36541979506862843No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16170.3629507423992458No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12440.2792274109738168No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12290.2758605209701132No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11740.26351525762320016No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.2621685016217187No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11020.24735418560542294No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9620.21592987890418952No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9170.2058292088930788No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8710.19550407954838783No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7860.17642503619406752No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7850.1762005768604873No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7380.16565098818221607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.16273301684567298No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7230.16228409817851253No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.15914166750838915No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6950.15599923683826583No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6760.15173450950024128No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6540.14679640416147605No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6480.14544964815999462No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6420.14410289215851319No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6410.14387843282493296No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6240.1400626241540689No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6230.13983816482048864No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6080.13647127481678506No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5840.13108425081085934No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5820.13063533214369888No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5780.1297374948093779No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.127941820140736No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5640.12659506413925456No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5460.12255479613481027No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5400.12120804013332884No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5280.11851452813036599No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5280.11851452813036599No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5190.11649439412814383No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5090.11424980079234145No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5060.11357642279160073No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5020.11267858545727977No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5010.11245412612369954No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4910.11020953278789716No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4830.10841385811925525No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4700.10549588678271216No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4700.10549588678271216No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4640.10414913078123073No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4600.10325129344690975No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4590.10302683411332952No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4530.1016800781118481No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4520.10145561877826785No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4510.10123115944468762No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4500.10100670011110736No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4460.10010886277678642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT251.2324123E-437.011
TAGCAAC200.001840726137.01
AGTGAGT251.2324123E-437.08
GTCGTAT251.2324123E-437.013
GGCCGCT303.595972E-430.83333229
TCGTATT303.595972E-430.83333214
TTCGCGG303.595972E-430.83333224
TAATGCC303.595972E-430.83333235
TCGCGGC303.595972E-430.83333225
AGTCGTA303.595972E-430.83333212
AGCAACG250.005492775729.62
GATTCTG250.005492775729.626
CTAATGC250.005492775729.634
ATCTCGT405.9320893E-527.75000237
CCTCACT905.456968E-1226.7222218
ACAACGC358.860835E-426.4285723
AACAACG358.860835E-426.4285722
TTTCGCG358.860835E-426.42857223
TCGGCGT509.07478E-625.90000211
CCGCTCT3600.025.18055528
Produced by FastQC (version 0.11.5)