##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633214.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445515
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91922381962448	33.0	33.0	33.0	33.0	33.0
2	32.00347014129715	33.0	33.0	33.0	33.0	33.0
3	31.713206064891192	33.0	33.0	33.0	27.0	33.0
4	31.98312963648811	33.0	33.0	33.0	33.0	33.0
5	32.09866558926186	33.0	33.0	33.0	33.0	33.0
6	35.21135764227916	37.0	37.0	37.0	33.0	37.0
7	35.39811454159793	37.0	37.0	37.0	33.0	37.0
8	35.56134361357081	37.0	37.0	37.0	33.0	37.0
9	35.67065530902439	37.0	37.0	37.0	33.0	37.0
10	35.625644478861545	37.0	37.0	37.0	33.0	37.0
11	35.65940765181868	37.0	37.0	37.0	33.0	37.0
12	35.65437751815315	37.0	37.0	37.0	33.0	37.0
13	35.665607218612166	37.0	37.0	37.0	33.0	37.0
14	35.64079548387821	37.0	37.0	37.0	33.0	37.0
15	35.683141981751454	37.0	37.0	37.0	33.0	37.0
16	35.67486616612235	37.0	37.0	37.0	33.0	37.0
17	35.67518938756271	37.0	37.0	37.0	33.0	37.0
18	35.641836975186024	37.0	37.0	37.0	33.0	37.0
19	35.62417875941326	37.0	37.0	37.0	33.0	37.0
20	35.61495796998979	37.0	37.0	37.0	33.0	37.0
21	35.61113542753892	37.0	37.0	37.0	33.0	37.0
22	35.514795236972944	37.0	37.0	37.0	33.0	37.0
23	35.56242999674534	37.0	37.0	37.0	33.0	37.0
24	35.57164854157548	37.0	37.0	37.0	33.0	37.0
25	35.581681873786515	37.0	37.0	37.0	33.0	37.0
26	35.4803609306084	37.0	37.0	37.0	33.0	37.0
27	35.482515740210765	37.0	37.0	37.0	33.0	37.0
28	35.493242651762564	37.0	37.0	37.0	33.0	37.0
29	35.48529005757382	37.0	37.0	37.0	33.0	37.0
30	35.48135304086282	37.0	37.0	37.0	33.0	37.0
31	35.43408863899083	37.0	37.0	37.0	33.0	37.0
32	35.39993266219992	37.0	37.0	37.0	33.0	37.0
33	35.347754845515865	37.0	37.0	37.0	33.0	37.0
34	35.28331930462498	37.0	37.0	37.0	33.0	37.0
35	35.18689606410558	37.0	37.0	37.0	33.0	37.0
36	35.164759884627905	37.0	37.0	37.0	33.0	37.0
37	35.096398549992706	37.0	37.0	37.0	33.0	37.0
38	35.02871508254492	37.0	37.0	37.0	33.0	37.0
39	34.87753498759862	37.0	37.0	37.0	27.0	37.0
40	34.58716317071254	37.0	37.0	37.0	27.0	37.0
41	34.680304815775	37.0	37.0	37.0	27.0	37.0
42	34.623238274805566	37.0	37.0	37.0	27.0	37.0
43	34.21426439064902	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	23.0
16	25.0
17	15.0
18	26.0
19	20.0
20	49.0
21	81.0
22	205.0
23	477.0
24	977.0
25	1756.0
26	2830.0
27	4491.0
28	6396.0
29	8882.0
30	12209.0
31	15737.0
32	19331.0
33	25291.0
34	35406.0
35	69241.0
36	242043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71540801095362	18.31677945748179	14.439693388550328	21.528119143014266
2	16.484966836133463	23.241866154899384	38.86378685341683	21.409380155550316
3	19.020908386923	29.502710346452982	29.951853472947036	21.52452779367698
4	11.46830073061513	18.05505987452723	40.53825348192541	29.938385912932226
5	14.155976790904909	36.47733521879174	32.4545750423667	16.91211294793666
6	25.65547736888769	42.0320303469019	16.495516424811736	15.816975859398674
7	25.049661627554627	33.72030122442566	21.67626230317722	19.553774844842486
8	25.681514651582997	31.826088908342033	19.716058943020997	22.776337497053973
9	28.782195885660418	12.631224537894347	19.117874819029662	39.46870475741557
10	17.031300854067762	26.431657744408156	30.568667721625538	25.968373679898544
11	34.132184101545405	24.61017025240452	21.899150421422398	19.358495224627678
12	24.554504337676622	27.01816998305332	27.760008080536007	20.66731759873405
13	31.39086226052995	22.819658148434957	25.02990920619957	20.759570384835527
14	23.98886681705442	21.181553931966377	30.233101017923076	24.596478233056125
15	26.335589149635812	26.175325185459524	27.362939519432565	20.12614614547209
16	20.277431736305175	27.641942471072806	26.442656251753586	25.637969540868433
17	21.829343568678944	25.03237825886895	25.980718943245456	27.157559229206647
18	24.804552035285006	22.13528164034881	30.105159197782346	22.95500712658384
19	26.773284850117278	23.01785573998631	31.099065126875637	19.109794283020772
20	26.408089514382233	21.765372658608577	30.743970461151697	21.08256736585749
21	22.63313244222978	25.18590844303783	31.040930159478357	21.14002895525403
22	22.68116673961595	24.55383095967588	31.032625164135887	21.732377136572282
23	22.0706373522777	24.50691895895761	32.15604412870498	21.266399560059707
24	21.838770860689312	24.83777201665488	31.12846929957465	22.194987823081153
25	22.045497906916715	25.00746327284154	32.63189791589509	20.315140904346656
26	22.23718617779424	25.284221631145975	31.150915232932675	21.32767695812711
27	22.694409840297183	24.784126235929204	31.745508007586725	20.775955916186888
28	21.350571810152296	24.886704151375376	31.200071826986747	22.562652211485585
29	22.76646128637644	24.96795843013142	30.275972750636903	21.989607532855235
30	23.165325522148525	24.18728886793935	31.550452846705497	21.096932763206627
31	22.550531407472253	24.499511800949463	31.021177738123296	21.928779053454992
32	20.389436943761712	24.743050177884022	31.833720525683763	23.033792352670503
33	20.511767280562946	24.836425260653403	32.18477492340325	22.4670325353804
34	20.92297677968194	24.908252247399076	31.52621123867883	22.64255973424015
35	21.12723477323996	25.687126134922504	30.996262752095888	22.18937633974165
36	21.87558219139647	25.36368023523338	31.165729548948967	21.595008024421176
37	22.35278273458806	25.392186570598074	30.575177042299362	21.679853652514506
38	21.932819321459434	25.66266006756226	29.597656644557425	22.806863966420885
39	21.48659416630192	24.942594525436853	30.33590339270283	23.2349079155584
40	21.274480096068597	26.325039560957542	30.92039549734577	21.480084845628095
41	22.209128761096707	25.093880116269933	29.99674533966309	22.70024578297027
42	20.343647239711345	26.89426843091703	30.183271045868267	22.57881328350336
43	21.35371424082242	26.081950102690143	29.668136875301617	22.89619878118582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	165.0
2	168.0
3	644.0
4	1120.0
5	1120.0
6	1378.5
7	1637.0
8	1570.0
9	1503.0
10	2126.5
11	2750.0
12	2750.0
13	4341.0
14	5932.0
15	9873.0
16	13814.0
17	14515.0
18	15216.0
19	15216.0
20	11596.0
21	7976.0
22	5635.0
23	3294.0
24	3074.5
25	2855.0
26	2855.0
27	3073.0
28	3291.0
29	3785.0
30	4279.0
31	5285.5
32	6292.0
33	6292.0
34	9423.0
35	12554.0
36	13530.0
37	14506.0
38	16963.5
39	19421.0
40	19421.0
41	22766.5
42	26112.0
43	28669.5
44	31227.0
45	34780.5
46	38334.0
47	38334.0
48	40107.0
49	41880.0
50	43662.5
51	45445.0
52	43953.5
53	42462.0
54	42462.0
55	37486.5
56	32511.0
57	28001.0
58	23491.0
59	21243.0
60	18995.0
61	18995.0
62	15458.5
63	11922.0
64	8692.5
65	5463.0
66	5081.5
67	4700.0
68	4700.0
69	3725.0
70	2750.0
71	2147.0
72	1544.0
73	1410.0
74	1276.0
75	1276.0
76	781.0
77	286.0
78	204.5
79	123.0
80	99.0
81	75.0
82	75.0
83	76.0
84	77.0
85	54.5
86	32.0
87	28.0
88	24.0
89	24.0
90	17.0
91	10.0
92	7.5
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	445515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.81967248021815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09348911055585	21.447156174129283
2	8.664836724900008	4.821058395625687
3	3.505507081973956	2.925661766928022
4	2.0576816537038347	2.289761186983774
5	1.3602200954812533	1.8920438778649769
6	1.0100176126843852	1.6859015510478854
7	0.7161481974180484	1.3946105809628102
8	0.5905969201324116	1.3144170308727399
9	0.4731164772150062	1.1845750897004461
>10	3.6553764425391733	20.448180325732697
>50	0.507713465643252	9.873441275204259
>100	0.33069767730487576	18.527839697821463
>500	0.025747751031036558	4.607561160053605
>1k	0.007241554977479032	3.408440234363401
>5k	8.046172197198924E-4	1.8050608819798035
>10k+	8.046172197198924E-4	2.3742907707291385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10607	2.380840151285591	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8064	1.810040065991044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4005	0.8989596309888557	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2060	0.46238622717529154	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1628	0.36541979506862843	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1617	0.3629507423992458	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1244	0.2792274109738168	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1229	0.2758605209701132	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1174	0.26351525762320016	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1168	0.2621685016217187	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1102	0.24735418560542294	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	962	0.21592987890418952	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	917	0.2058292088930788	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	871	0.19550407954838783	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	786	0.17642503619406752	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	785	0.1762005768604873	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	738	0.16565098818221607	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.16273301684567298	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	723	0.16228409817851253	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.15914166750838915	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	695	0.15599923683826583	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	676	0.15173450950024128	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	654	0.14679640416147605	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	648	0.14544964815999462	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	642	0.14410289215851319	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	641	0.14387843282493296	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	624	0.1400626241540689	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	623	0.13983816482048864	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	608	0.13647127481678506	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	584	0.13108425081085934	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	582	0.13063533214369888	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	578	0.1297374948093779	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	570	0.127941820140736	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	564	0.12659506413925456	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	546	0.12255479613481027	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	540	0.12120804013332884	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	528	0.11851452813036599	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	528	0.11851452813036599	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	519	0.11649439412814383	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	509	0.11424980079234145	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	506	0.11357642279160073	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	502	0.11267858545727977	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	501	0.11245412612369954	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	491	0.11020953278789716	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	483	0.10841385811925525	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	470	0.10549588678271216	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	470	0.10549588678271216	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	464	0.10414913078123073	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	460	0.10325129344690975	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	459	0.10302683411332952	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	453	0.1016800781118481	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	452	0.10145561877826785	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	451	0.10123115944468762	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	450	0.10100670011110736	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	446	0.10010886277678642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.978373343209545E-4	0.0	0.0
2	2.2445933358023861E-4	0.0	0.001795674668641909	2.2445933358023861E-4	0.0
3	2.2445933358023861E-4	0.0	0.0026935120029628634	2.2445933358023861E-4	0.0
4	2.2445933358023861E-4	0.0	0.003591349337283818	6.733780007407158E-4	0.0
5	4.4891866716047723E-4	0.0	0.004040268004444294	6.733780007407158E-4	0.0
6	4.4891866716047723E-4	0.0	0.007856076675308351	8.978373343209545E-4	0.0
7	4.4891866716047723E-4	0.0	0.021548096023702907	0.0013467560014814317	0.0
8	6.733780007407158E-4	0.0	0.025363904694566963	0.0013467560014814317	0.0
9	6.733780007407158E-4	0.0	0.029853091366171734	0.0026935120029628634	0.0
10	0.0031424306701233404	0.0	0.03501565603851722	0.0029179713365431017	0.0
11	0.003366890003703579	0.0	0.05476807739357822	0.003591349337283818	0.0
12	0.003366890003703579	0.0	0.058808345398022516	0.003591349337283818	0.0
13	0.003591349337283818	0.0	0.06374645073678777	0.003591349337283818	0.0
14	0.003591349337283818	0.0	0.06868455607555302	0.003591349337283818	0.0
15	0.003591349337283818	0.0	0.07272482407999731	0.003591349337283818	0.0
16	0.003591349337283818	0.0	0.07878522608666375	0.004040268004444294	0.0
17	0.003591349337283818	0.0	0.07990752275456495	0.0047136460051850106	0.0
18	0.003591349337283818	0.0	0.08215211609036732	0.004938105338765249	0.0
19	0.003591349337283818	0.0	0.083723331425429	0.004938105338765249	0.0
20	0.003591349337283818	0.0	0.08641684342839186	0.005162564672345488	0.0
21	0.003591349337283818	0.0	0.08866143676419425	0.005611483339505965	0.0
22	0.003591349337283818	0.0	0.09023265209925592	0.007182698674567636	0.0
23	0.003591349337283818	0.0	0.09225278610147807	0.009427292010370021	0.0
24	0.003591349337283818	0.0	0.09270170476863854	0.014365397349135271	0.0
25	0.003591349337283818	0.0	0.09292616410221878	0.016385531351357417	0.0
26	0.003591349337283818	0.0	0.09292616410221878	0.018405665353579567	0.0
27	0.003591349337283818	0.0	0.09315062343579902	0.02401714869308553	0.0
28	0.003815808670864056	0.0	0.09315062343579902	0.05925726406518299	0.0
29	0.003815808670864056	0.0	0.09315062343579902	0.15173450950024128	0.0
30	0.003815808670864056	0.0	0.09337508276937925	0.25610809961505226	0.0
31	0.003815808670864056	0.0	0.09337508276937925	0.42355476246591023	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTCGT	25	1.2324123E-4	37.0	11
TAGCAAC	20	0.0018407261	37.0	1
AGTGAGT	25	1.2324123E-4	37.0	8
GTCGTAT	25	1.2324123E-4	37.0	13
GGCCGCT	30	3.595972E-4	30.833332	29
TCGTATT	30	3.595972E-4	30.833332	14
TTCGCGG	30	3.595972E-4	30.833332	24
TAATGCC	30	3.595972E-4	30.833332	35
TCGCGGC	30	3.595972E-4	30.833332	25
AGTCGTA	30	3.595972E-4	30.833332	12
AGCAACG	25	0.0054927757	29.6	2
GATTCTG	25	0.0054927757	29.6	26
CTAATGC	25	0.0054927757	29.6	34
ATCTCGT	40	5.9320893E-5	27.750002	37
CCTCACT	90	5.456968E-12	26.722221	8
ACAACGC	35	8.860835E-4	26.428572	3
AACAACG	35	8.860835E-4	26.428572	2
TTTCGCG	35	8.860835E-4	26.428572	23
TCGGCGT	50	9.07478E-6	25.900002	11
CCGCTCT	360	0.0	25.180555	28
>>END_MODULE