FastQCFastQC Report
Fri 10 Feb 2017
ERR1633213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338787
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11970.3533193422415854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.27687012783843534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5420.15998252589385073No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4390.12957994255977945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2350.027.553191
CGCAAGT358.856433E-426.42857212
CGAGTTC400.001928392423.12514
GGGTAAA502.6966535E-422.21
GTATTGA705.0844137E-621.1428591
CTCTAGT705.0844137E-621.14285927
AACGGCC806.9303496E-720.812537
ATCTTAC806.9303496E-720.81251
TATACTG450.003820322220.5555575
TAACTAG1351.8189894E-1220.55555326
GTATCAA5700.020.4473691
ACCCCGC555.132808E-420.1818186
TGCGTCA555.132808E-420.18181810
GCCGTTA656.883779E-519.92307715
TTAACGG851.2400105E-619.58823635
CCCCTAT1551.8189894E-1219.0967731
TACCCCG609.218494E-418.55
TATATAG902.1431115E-618.52
TAACGGC902.1431115E-618.536
CCGAGTT500.00702489218.513
Produced by FastQC (version 0.11.5)