##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633213.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338787
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.855626101355718	33.0	33.0	33.0	33.0	33.0
2	31.97460351194113	33.0	33.0	33.0	33.0	33.0
3	31.682366796836952	33.0	33.0	33.0	27.0	33.0
4	31.955656503939053	33.0	33.0	33.0	33.0	33.0
5	32.06901091246122	33.0	33.0	33.0	33.0	33.0
6	35.24178613701234	37.0	37.0	37.0	33.0	37.0
7	35.398855327978936	37.0	37.0	37.0	33.0	37.0
8	35.52287130261787	37.0	37.0	37.0	33.0	37.0
9	35.650447036043296	37.0	37.0	37.0	33.0	37.0
10	35.64762520403676	37.0	37.0	37.0	33.0	37.0
11	35.66622981401294	37.0	37.0	37.0	33.0	37.0
12	35.67319879452281	37.0	37.0	37.0	33.0	37.0
13	35.6566751380661	37.0	37.0	37.0	33.0	37.0
14	35.65157163645594	37.0	37.0	37.0	33.0	37.0
15	35.646199529497885	37.0	37.0	37.0	33.0	37.0
16	35.63807643150416	37.0	37.0	37.0	33.0	37.0
17	35.63436908736168	37.0	37.0	37.0	33.0	37.0
18	35.60895784076721	37.0	37.0	37.0	33.0	37.0
19	35.604314805467745	37.0	37.0	37.0	33.0	37.0
20	35.59710968838828	37.0	37.0	37.0	33.0	37.0
21	35.62096833703772	37.0	37.0	37.0	33.0	37.0
22	35.52603258094319	37.0	37.0	37.0	33.0	37.0
23	35.55879357826599	37.0	37.0	37.0	33.0	37.0
24	35.559673186987695	37.0	37.0	37.0	33.0	37.0
25	35.58295034933454	37.0	37.0	37.0	33.0	37.0
26	35.48065598739031	37.0	37.0	37.0	33.0	37.0
27	35.479419222107104	37.0	37.0	37.0	33.0	37.0
28	35.49377632553787	37.0	37.0	37.0	33.0	37.0
29	35.517047584470475	37.0	37.0	37.0	33.0	37.0
30	35.51872415411453	37.0	37.0	37.0	33.0	37.0
31	35.52080215592688	37.0	37.0	37.0	33.0	37.0
32	35.49353133384693	37.0	37.0	37.0	33.0	37.0
33	35.49638268292467	37.0	37.0	37.0	33.0	37.0
34	35.469430645213656	37.0	37.0	37.0	33.0	37.0
35	35.41266341388542	37.0	37.0	37.0	33.0	37.0
36	35.444190597632144	37.0	37.0	37.0	33.0	37.0
37	35.44036518520486	37.0	37.0	37.0	33.0	37.0
38	35.418245092048984	37.0	37.0	37.0	33.0	37.0
39	35.33556482391591	37.0	37.0	37.0	33.0	37.0
40	35.12806571680731	37.0	37.0	37.0	33.0	37.0
41	35.295929300711066	37.0	37.0	37.0	33.0	37.0
42	35.3310782290938	37.0	37.0	37.0	33.0	37.0
43	35.00699259416684	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	12.0
17	16.0
18	3.0
19	13.0
20	12.0
21	52.0
22	122.0
23	301.0
24	713.0
25	1289.0
26	2107.0
27	3135.0
28	4516.0
29	6248.0
30	8202.0
31	10444.0
32	13529.0
33	17924.0
34	25955.0
35	53512.0
36	190673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.393521593213435	21.184106828184078	13.608845675896655	24.81352590270583
2	15.9867409316178	21.63394699324354	38.34710304704726	24.032209028091398
3	17.74448252146629	26.618494806471322	30.269166172255723	25.367856499806663
4	12.602018377328527	17.76071691062526	39.57353735532945	30.063727356716758
5	13.940912726875588	37.21394268375115	35.32573563920693	13.519408950166328
6	29.84028312774693	39.8566060681195	17.225277239091227	13.077833565042344
7	26.980964440784327	31.967578448995976	23.514479599276243	17.536977510943455
8	24.636423475517066	35.94854584148742	20.503147995643282	18.91188268735223
9	25.58746350952073	15.275084345030947	20.594946087069456	38.54250605837886
10	14.561361563460226	28.38833839551104	34.01783421441791	23.03246582661082
11	33.70141121117398	23.403200240859302	24.67715703377048	18.218231514196233
12	22.604763464949954	25.944620071018072	30.47519532921865	20.975421134813317
13	27.53854191571696	22.184145200376637	26.519022276533633	23.75829060737277
14	21.54569095036114	20.981619719764925	27.913999061357135	29.558690268516795
15	23.56318276675315	29.19799165847568	24.28103793829161	22.95778763647956
16	23.007966657516373	28.66697954762137	25.06678237358576	23.258271421276493
17	22.294243875945654	28.02380256621417	26.736267920551853	22.945685637288328
18	22.547205176113604	27.608497374456515	28.001664762815576	21.8426326866143
19	24.077665317736514	26.822752939162363	27.48600152898429	21.613580214116833
20	23.498245210117272	26.836921133337466	27.571010694034896	22.093822962510366
21	22.787178964954382	27.027896583989353	27.790617703748964	22.394306747307304
22	22.48551449730952	27.15039242946158	27.63211103141502	22.731982041813882
23	22.526543226274917	26.835740450489542	27.94735335181102	22.690362971424523
24	22.505290935012265	27.566878304067156	27.09312931133721	22.834701449583367
25	22.861266813661683	27.03468551036492	27.697048588050897	22.4069990879225
26	22.98612402482976	27.07158184936258	27.578980303258387	22.363313822549273
27	22.52034464132331	27.421359143060386	27.097261701304948	22.96103451431135
28	22.34265187271058	26.95882663738573	27.93849823045158	22.760023259452105
29	23.195400059624482	26.86201064385587	27.253997349367005	22.688591947152638
30	22.925318858161617	26.90599107994108	27.571896206170837	22.59679385572646
31	22.769468722235505	26.905105567805137	27.642146835622384	22.683278874336974
32	22.275352950378853	26.931375761171473	27.73984834128818	23.053422947161494
33	22.142821300699257	26.982440294344233	28.27027011071853	22.60446829423797
34	22.968118611398904	26.630006464238594	27.736306292744406	22.665568631618097
35	22.64549702320337	27.444677629306906	27.4464486535788	22.463376693910924
36	23.602440471446663	26.82836118269001	27.258424910046724	22.310773435816607
37	22.87720603210867	26.542340762780153	27.387709681894524	23.192743523216652
38	22.844442083078746	26.999265024927166	27.397745486101886	22.758547405892198
39	22.568457467376255	26.916026884148447	27.499874552447405	23.01564109602789
40	22.578788442295604	27.510795868790716	27.616171812968034	22.294243875945654
41	22.763860478707862	26.512823691582028	27.59373883885745	23.12957699085266
42	22.525952884850952	27.15304896586941	27.670778394684564	22.65021975459507
43	22.845917936638653	26.485372815367768	27.592262985297545	23.07644626269603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	22.0
2	27.0
3	94.5
4	162.0
5	162.0
6	193.5
7	225.0
8	204.5
9	184.0
10	246.0
11	308.0
12	308.0
13	515.0
14	722.0
15	1285.0
16	1848.0
17	2079.5
18	2311.0
19	2311.0
20	2390.0
21	2469.0
22	2746.5
23	3024.0
24	3948.5
25	4873.0
26	4873.0
27	6128.5
28	7384.0
29	8786.0
30	10188.0
31	11946.5
32	13705.0
33	13705.0
34	16080.5
35	18456.0
36	20307.5
37	22159.0
38	23267.5
39	24376.0
40	24376.0
41	25239.0
42	26102.0
43	26835.5
44	27569.0
45	26844.0
46	26119.0
47	26119.0
48	25262.5
49	24406.0
50	24470.5
51	24535.0
52	23419.5
53	22304.0
54	22304.0
55	21095.5
56	19887.0
57	17282.5
58	14678.0
59	13508.5
60	12339.0
61	12339.0
62	11025.5
63	9712.0
64	8193.5
65	6675.0
66	5651.0
67	4627.0
68	4627.0
69	3740.0
70	2853.0
71	2417.0
72	1981.0
73	1525.0
74	1069.0
75	1069.0
76	821.0
77	573.0
78	460.0
79	347.0
80	295.5
81	244.0
82	244.0
83	184.0
84	124.0
85	118.5
86	113.0
87	89.5
88	66.0
89	66.0
90	43.5
91	21.0
92	12.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	338787.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56331996645264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.31508410376598	56.591034770264784
2	7.517757224165451	9.857782446361405
3	2.2658431390004936	4.456685961482424
4	1.092740142747097	2.8657468647646023
5	0.6523520947459381	2.1385184559306785
6	0.4045603832057265	1.5914593109920643
7	0.29068738951607564	1.334090123033872
8	0.21500999850641078	1.127741546244985
9	0.17139353510022548	1.0113416263761763
>10	0.9734805821391499	11.83254880039302
>50	0.06876037150105742	3.0891333301888486
>100	0.030964935510402768	3.3046889757276787
>500	9.107333973647873E-4	0.44185921800315886
>1k	4.5536669868239364E-4	0.35736857023633856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.3533193422415854	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.27687012783843534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.15998252589385073	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	439	0.12957994255977945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	2.951707119812744E-4	2.951707119812744E-4	0.0
5	0.0	0.0	2.951707119812744E-4	2.951707119812744E-4	0.0
6	0.0	0.0	8.855121359438231E-4	2.951707119812744E-4	0.0
7	0.0	0.0	8.855121359438231E-4	2.951707119812744E-4	0.0
8	0.0	0.0	8.855121359438231E-4	2.951707119812744E-4	0.0
9	0.0	0.0	0.0011806828479250975	5.903414239625487E-4	0.0
10	0.0	0.0	0.0011806828479250975	5.903414239625487E-4	0.0
11	0.0	0.0	0.0017710242718876462	5.903414239625487E-4	0.0
12	0.0	0.0	0.0017710242718876462	8.855121359438231E-4	0.0
13	0.0	0.0	0.0020661949838689207	8.855121359438231E-4	0.0
14	0.0	0.0	0.0029517071198127435	8.855121359438231E-4	0.0
15	0.0	0.0	0.0032468778317940182	8.855121359438231E-4	0.0
16	0.0	0.0	0.0038372192557565668	8.855121359438231E-4	0.0
17	0.0	0.0	0.0038372192557565668	8.855121359438231E-4	0.0
18	0.0	0.0	0.0038372192557565668	8.855121359438231E-4	0.0
19	0.0	0.0	0.0038372192557565668	8.855121359438231E-4	0.0
20	0.0	0.0	0.0038372192557565668	0.0011806828479250975	0.0
21	0.0	0.0	0.0038372192557565668	0.0017710242718876462	0.0
22	0.0	0.0	0.0038372192557565668	0.0020661949838689207	0.0
23	0.0	0.0	0.0038372192557565668	0.002361365695850195	0.0
24	0.0	0.0	0.0038372192557565668	0.0029517071198127435	0.0
25	0.0	0.0	0.0038372192557565668	0.0035420485437752925	0.0
26	0.0	0.0	0.0038372192557565668	0.0038372192557565668	0.0
27	0.0	0.0	0.0038372192557565668	0.005017902103681664	0.0
28	0.0	0.0	0.0038372192557565668	0.013577852751138621	0.0
29	0.0	0.0	0.0038372192557565668	0.026860534790295966	0.0
30	0.0	0.0	0.0038372192557565668	0.042799753237284785	0.0
31	0.0	0.0	0.0038372192557565668	0.08648501861051339	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	235	0.0	27.55319	1
CGCAAGT	35	8.856433E-4	26.428572	12
CGAGTTC	40	0.0019283924	23.125	14
GGGTAAA	50	2.6966535E-4	22.2	1
GTATTGA	70	5.0844137E-6	21.142859	1
CTCTAGT	70	5.0844137E-6	21.142859	27
AACGGCC	80	6.9303496E-7	20.8125	37
ATCTTAC	80	6.9303496E-7	20.8125	1
TATACTG	45	0.0038203222	20.555557	5
TAACTAG	135	1.8189894E-12	20.555553	26
GTATCAA	570	0.0	20.447369	1
ACCCCGC	55	5.132808E-4	20.181818	6
TGCGTCA	55	5.132808E-4	20.181818	10
GCCGTTA	65	6.883779E-5	19.923077	15
TTAACGG	85	1.2400105E-6	19.588236	35
CCCCTAT	155	1.8189894E-12	19.096773	1
TACCCCG	60	9.218494E-4	18.5	5
TATATAG	90	2.1431115E-6	18.5	2
TAACGGC	90	2.1431115E-6	18.5	36
CCGAGTT	50	0.007024892	18.5	13
>>END_MODULE