FastQCFastQC Report
Fri 10 Feb 2017
ERR1633212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641169
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118881.8541133460912802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87521.3650067298949262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45480.7093293655806816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.3672978575071471No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18880.29446214648556No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15780.24611295929778265No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15750.24564506393790092No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14900.2323880287412523No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14540.22677328442267172No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14330.2234980169034997No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13210.20602992346791565No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13130.20478220250823106No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11910.1857544578730413No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.18513059739319898No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11590.18076357403430296No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11320.17655251579536754No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11190.1745249692358801No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11170.17421303899595894No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11160.17405707387599836No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11120.17343321339615608No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10570.16485513179832462No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10330.1611119689192709No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10020.15627705020049315No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9900.1544054687609663No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9600.14972651516214913No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9500.14816686396254342No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9060.14130439868427824No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9040.1409924684443571No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8820.13756123580522453No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8720.1360015846056188No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8690.13553368924573708No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG8670.13522175900581593No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8530.13303824732636793No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8370.13054280540699878No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.13007491004711705No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8270.12898315420739306No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8260.1288271890874325No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8230.12835929372755078No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8220.1282033286075902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.1264877122880239No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7880.12290051452893075No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7850.12243261916904903No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7740.12071700284948274No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7710.12024910748960102No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7650.11931331676983759No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7430.11588208413070501No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7420.11572611901074445No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7390.11525822365086272No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7390.11525822365086272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.11510225853090215No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7300.11385453757121757No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7280.11354260733129644No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7190.11213892125165129No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7080.11042330493208499No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6960.10855172349255812No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6950.10839575837259754No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6950.10839575837259754No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6870.10714803741291297No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6760.10543242109334668No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6740.10512049085342555No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6620.10324890941389867No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6540.1020011884542141No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6520.10168925821429295No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6510.10153329309433239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAT405.9360947E-527.7500025
ACCGCTT358.865102E-426.4285728
TAAGGAT358.865102E-426.4285724
ACAACGC509.0829035E-625.9000023
CTAGTGC509.0829035E-625.9000029
CTAAGCC451.3223207E-424.6666664
TAGTGCA551.9009582E-523.54545410
GCCGCTC3950.022.94936827
GACGTAT652.6797752E-622.7692328
ACGTATA652.6797752E-622.7692329
GCCGGCA4350.022.5402315
CCGCTCT4050.022.38271528
TTCGCCG4400.022.2840924
CGCTCTC4000.022.229
TCTAAGG502.7002968E-422.222
GCTCTCT4150.021.84337430
GGCAGCT4600.021.71739218
CCGGCAG4600.021.71739216
CGGCAGC4750.021.42105317
GTATCAA49600.021.4092751
Produced by FastQC (version 0.11.5)