##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633212.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641169
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916583615240288	33.0	33.0	33.0	33.0	33.0
2	32.012600422041615	33.0	33.0	33.0	33.0	33.0
3	31.71989444280681	33.0	33.0	33.0	27.0	33.0
4	32.00580970071853	33.0	33.0	33.0	33.0	33.0
5	32.108375482907	33.0	33.0	33.0	33.0	33.0
6	35.22359315562667	37.0	37.0	37.0	33.0	37.0
7	35.403210386029265	37.0	37.0	37.0	33.0	37.0
8	35.545233783916565	37.0	37.0	37.0	33.0	37.0
9	35.669598810921926	37.0	37.0	37.0	33.0	37.0
10	35.63156203746594	37.0	37.0	37.0	33.0	37.0
11	35.647370662025146	37.0	37.0	37.0	33.0	37.0
12	35.6424047326056	37.0	37.0	37.0	33.0	37.0
13	35.66627675386677	37.0	37.0	37.0	33.0	37.0
14	35.64384740996523	37.0	37.0	37.0	33.0	37.0
15	35.66672281410985	37.0	37.0	37.0	33.0	37.0
16	35.655237542675955	37.0	37.0	37.0	33.0	37.0
17	35.652310077374295	37.0	37.0	37.0	33.0	37.0
18	35.62769722179332	37.0	37.0	37.0	33.0	37.0
19	35.621059346287794	37.0	37.0	37.0	33.0	37.0
20	35.604085662282486	37.0	37.0	37.0	33.0	37.0
21	35.60222655805256	37.0	37.0	37.0	33.0	37.0
22	35.51030071634779	37.0	37.0	37.0	33.0	37.0
23	35.55862338946518	37.0	37.0	37.0	33.0	37.0
24	35.54901749772681	37.0	37.0	37.0	33.0	37.0
25	35.56857708342106	37.0	37.0	37.0	33.0	37.0
26	35.46449064131298	37.0	37.0	37.0	33.0	37.0
27	35.47161200869038	37.0	37.0	37.0	33.0	37.0
28	35.490026810404125	37.0	37.0	37.0	33.0	37.0
29	35.48172322741742	37.0	37.0	37.0	33.0	37.0
30	35.470673098668215	37.0	37.0	37.0	33.0	37.0
31	35.42691708426327	37.0	37.0	37.0	33.0	37.0
32	35.39964658303817	37.0	37.0	37.0	33.0	37.0
33	35.3722060798323	37.0	37.0	37.0	33.0	37.0
34	35.31348989112075	37.0	37.0	37.0	33.0	37.0
35	35.21049676450359	37.0	37.0	37.0	33.0	37.0
36	35.196787430459054	37.0	37.0	37.0	33.0	37.0
37	35.153341786642834	37.0	37.0	37.0	33.0	37.0
38	35.0897922388637	37.0	37.0	37.0	33.0	37.0
39	34.94956867846075	37.0	37.0	37.0	27.0	37.0
40	34.67437602254632	37.0	37.0	37.0	27.0	37.0
41	34.79401998537047	37.0	37.0	37.0	27.0	37.0
42	34.74396609942153	37.0	37.0	37.0	27.0	37.0
43	34.35530258013098	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	18.0
16	38.0
17	37.0
18	18.0
19	33.0
20	44.0
21	137.0
22	312.0
23	686.0
24	1491.0
25	2550.0
26	4273.0
27	6527.0
28	9442.0
29	12772.0
30	17033.0
31	21708.0
32	27675.0
33	35384.0
34	50145.0
35	98611.0
36	352233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77789787092015	19.22737998873932	13.825528058904906	22.169194081435627
2	16.46804508639688	22.452894634643908	39.00250947878017	22.07655080017905
3	18.299075594733992	29.49362804502401	29.85094413485368	22.356352225388314
4	11.470142817260347	18.03065962328185	40.2060923095159	30.293105249941902
5	13.919107130881251	36.75941912350722	33.560262582875964	15.761211162735567
6	26.9014877512793	41.71910993825341	16.3710347817814	15.008367528685884
7	25.938871030882655	33.167386445695286	22.203194477587033	18.69054804583503
8	25.06983338246235	32.94373246367183	20.255190129279487	21.731244024586342
9	28.00400518428059	13.374788862218853	19.92236056328363	38.69884539021693
10	16.80149851287258	26.770477050512426	31.41168709029912	25.01633734631587
11	33.753971261866994	24.251796328269144	22.41234370345416	19.581888706409696
12	24.728269769748692	27.08303115091341	27.731066224349586	20.45763285498831
13	29.08406364000755	23.259702200199946	25.611968139445295	22.04426602034721
14	23.338620550899996	20.990565669893584	29.59625309395807	26.07456068524835
15	25.811447527874865	26.601878755835045	27.71328620067408	19.87338751561601
16	21.153705185372342	28.158410653041553	26.442950298595225	24.24493386299088
17	21.989678228361008	25.66499628023189	26.123065837556087	26.22225965385101
18	23.314445957306106	24.309347457534596	30.167397363253684	22.20880922190561
19	25.81628244659364	23.880287412523064	30.189076514928203	20.114353625955093
20	24.95629077513105	23.070984405047657	30.72044967863387	21.252275141187425
21	22.12848718512592	26.063487161731153	30.56588824475294	21.24213740838999
22	22.111019091690334	25.84014510994761	29.952009532588132	22.096826265773924
23	21.93649412245445	25.79959417875786	31.261804610017013	21.002107088770668
24	22.651594197473678	26.20915858377432	28.808473273037215	22.33077394571478
25	22.127083499046275	25.983321090071414	31.110830373895183	20.778765036987128
26	22.492977670473778	26.347655610299313	30.32492213441386	20.834444584813053
27	23.002359752265004	25.521040474508283	30.053698790802425	21.422900982424288
28	21.088667730348785	25.88069604113736	30.721853364713514	22.30878286380034
29	23.94704048386619	25.457562670684332	29.221936806052696	21.37346003939679
30	23.016864508421335	24.77615106157659	30.97919581264846	21.227788617353617
31	22.151570022880083	25.558628068418777	30.217150236521107	22.072651672180033
32	20.162390882902947	25.42449806525269	30.846469495562012	23.566641556282352
33	20.5201748680925	25.380048006063927	31.7697830057286	22.329994120114975
34	21.30265187493469	25.596059697209316	31.049848011990598	22.051440415865393
35	21.301716084214924	26.745522631318728	29.904908066360044	22.047853218106304
36	22.428252145690138	26.181240827301384	29.980083254181032	21.410423772827443
37	21.88065860950857	26.007495683665304	29.67579530513796	22.436050401688163
38	22.012449135875254	26.028706939979944	28.982062451553336	22.97678147259147
39	21.56498520670837	25.964449310556187	29.70605253841031	22.76451294432513
40	21.390460237472492	27.818094761287586	29.69279550321366	21.098649498026262
41	22.1024410100925	25.443213879647956	29.39490212408897	23.059442986170573
42	20.500211332737546	27.381548390517946	29.52326141781652	22.594978858927988
43	22.158276523038385	25.96429334543623	28.883960391098135	22.99346974042725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	88.0
2	164.0
3	708.5
4	1253.0
5	1253.0
6	1617.5
7	1982.0
8	1912.0
9	1842.0
10	2503.5
11	3165.0
12	3165.0
13	4952.0
14	6739.0
15	11121.0
16	15503.0
17	16463.0
18	17423.0
19	17423.0
20	13465.5
21	9508.0
22	6915.0
23	4322.0
24	4457.5
25	4593.0
26	4593.0
27	5345.5
28	6098.0
29	6973.5
30	7849.0
31	10359.0
32	12869.0
33	12869.0
34	18605.0
35	24341.0
36	26651.0
37	28961.0
38	32834.0
39	36707.0
40	36707.0
41	39450.5
42	42194.0
43	46692.5
44	51191.0
45	52360.0
46	53529.0
47	53529.0
48	54597.5
49	55666.0
50	58479.0
51	61292.0
52	58025.0
53	54758.0
54	54758.0
55	50693.0
56	46628.0
57	38779.0
58	30930.0
59	27479.5
60	24029.0
61	24029.0
62	19731.0
63	15433.0
64	11693.5
65	7954.0
66	6968.5
67	5983.0
68	5983.0
69	4811.0
70	3639.0
71	2876.0
72	2113.0
73	1804.5
74	1496.0
75	1496.0
76	978.0
77	460.0
78	345.0
79	230.0
80	170.0
81	110.0
82	110.0
83	97.0
84	84.0
85	75.5
86	67.0
87	55.0
88	43.0
89	43.0
90	25.5
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	641169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.879561497784007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11720026437247	21.77873288805281
2	10.01588979409092	5.5847723093877
3	3.356057430067806	2.8069622853501106
4	1.7227673330268003	1.9211999122997605
5	1.1058785297920126	1.5415704240207688
6	0.7614115425360588	1.273669195515398
7	0.5502637407620175	1.073877826040274
8	0.4315741735441308	0.9625678969743123
9	0.34039280553486706	0.8541001939781301
>10	2.741948312951019	15.657123995002326
>50	0.39818590414292654	7.679216599996833
>100	0.40048092376334693	22.196258350493896
>500	0.04475288259819636	8.380802702181642
>1k	0.012048853007206713	4.987563763365785
>5k	5.737549051050815E-4	1.399972997337311
>10k+	5.737549051050815E-4	1.9016086600029651
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11888	1.8541133460912802	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8752	1.3650067298949262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4548	0.7093293655806816	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2355	0.3672978575071471	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1888	0.29446214648556	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1578	0.24611295929778265	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1575	0.24564506393790092	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1490	0.2323880287412523	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1454	0.22677328442267172	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1433	0.2234980169034997	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1321	0.20602992346791565	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1313	0.20478220250823106	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1191	0.1857544578730413	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1187	0.18513059739319898	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1159	0.18076357403430296	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1132	0.17655251579536754	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1119	0.1745249692358801	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1117	0.17421303899595894	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1116	0.17405707387599836	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1112	0.17343321339615608	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1057	0.16485513179832462	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1033	0.1611119689192709	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1002	0.15627705020049315	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	990	0.1544054687609663	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	960	0.14972651516214913	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	950	0.14816686396254342	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	906	0.14130439868427824	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	904	0.1409924684443571	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	882	0.13756123580522453	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	872	0.1360015846056188	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	869	0.13553368924573708	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	867	0.13522175900581593	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	853	0.13303824732636793	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	837	0.13054280540699878	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	834	0.13007491004711705	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	827	0.12898315420739306	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	826	0.1288271890874325	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	823	0.12835929372755078	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	822	0.1282033286075902	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.1264877122880239	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	788	0.12290051452893075	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	785	0.12243261916904903	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	774	0.12071700284948274	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	771	0.12024910748960102	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	765	0.11931331676983759	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	743	0.11588208413070501	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	742	0.11572611901074445	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	739	0.11525822365086272	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	739	0.11525822365086272	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.11510225853090215	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	730	0.11385453757121757	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	728	0.11354260733129644	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	719	0.11213892125165129	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	708	0.11042330493208499	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	696	0.10855172349255812	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	695	0.10839575837259754	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	695	0.10839575837259754	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	687	0.10714803741291297	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	676	0.10543242109334668	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	674	0.10512049085342555	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	662	0.10324890941389867	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	654	0.1020011884542141	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	652	0.10168925821429295	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	651	0.10153329309433239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.798255998028601E-4	0.0	0.0
2	1.55965119960572E-4	0.0	9.35790719763432E-4	1.55965119960572E-4	0.0
3	1.55965119960572E-4	0.0	0.0010917558397240041	1.55965119960572E-4	0.0
4	1.55965119960572E-4	0.0	0.0017156163195662922	3.11930239921144E-4	0.0
5	1.55965119960572E-4	0.0	0.0029633372792508684	3.11930239921144E-4	0.0
6	1.55965119960572E-4	0.0	0.005458779198620021	3.11930239921144E-4	0.0
7	1.55965119960572E-4	0.0	0.01232124447688519	3.11930239921144E-4	0.0
8	1.55965119960572E-4	0.0	0.015752477116017773	3.11930239921144E-4	0.0
9	3.11930239921144E-4	0.0	0.021367221434598366	0.001871581439526864	0.0
10	0.0017156163195662922	0.0	0.02417459359388866	0.001871581439526864	0.0
11	0.0017156163195662922	0.0	0.039459175350024724	0.001871581439526864	0.0
12	0.0017156163195662922	0.0	0.04304637310911788	0.001871581439526864	0.0
13	0.0017156163195662922	0.0	0.04616567550832932	0.001871581439526864	0.0
14	0.0017156163195662922	0.0	0.04928497790754076	0.001871581439526864	0.0
15	0.0017156163195662922	0.0	0.05427586174627906	0.0020275465594874363	0.0
16	0.001871581439526864	0.0	0.05770709438541165	0.002495441919369152	0.0
17	0.0021835116794480082	0.0	0.05973464094489908	0.002495441919369152	0.0
18	0.0021835116794480082	0.0	0.06082639678462309	0.0031193023992114404	0.0
19	0.00233947679940858	0.0	0.06191815262434709	0.0032752675191720123	0.0
20	0.00233947679940858	0.0	0.06316587358403167	0.0034312326391325843	0.0
21	0.00233947679940858	0.0	0.06488148990359796	0.004834918718777732	0.0
22	0.00233947679940858	0.0	0.06612921086328254	0.0070184303982257406	0.0
23	0.00233947679940858	0.0	0.06644114110320368	0.010293697917397752	0.0
24	0.00233947679940858	0.0	0.06659710622316425	0.013413000316609193	0.0
25	0.00233947679940858	0.0	0.06659710622316425	0.016532302715820634	0.0
26	0.00233947679940858	0.0	0.06659710622316425	0.01933967487511093	0.0
27	0.00233947679940858	0.0	0.06659710622316425	0.02479845407373095	0.0
28	0.002651407039329724	0.0	0.06675307134312482	0.05567954782592421	0.0
29	0.002651407039329724	0.0	0.06675307134312482	0.1197812121297193	0.0
30	0.002651407039329724	0.0	0.06675307134312482	0.2044702722683099	0.0
31	0.002651407039329724	0.0	0.06675307134312482	0.3373525544747173	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGAT	40	5.9360947E-5	27.750002	5
ACCGCTT	35	8.865102E-4	26.42857	28
TAAGGAT	35	8.865102E-4	26.42857	24
ACAACGC	50	9.0829035E-6	25.900002	3
CTAGTGC	50	9.0829035E-6	25.900002	9
CTAAGCC	45	1.3223207E-4	24.666666	4
TAGTGCA	55	1.9009582E-5	23.545454	10
GCCGCTC	395	0.0	22.949368	27
GACGTAT	65	2.6797752E-6	22.76923	28
ACGTATA	65	2.6797752E-6	22.76923	29
GCCGGCA	435	0.0	22.54023	15
CCGCTCT	405	0.0	22.382715	28
TTCGCCG	440	0.0	22.28409	24
CGCTCTC	400	0.0	22.2	29
TCTAAGG	50	2.7002968E-4	22.2	22
GCTCTCT	415	0.0	21.843374	30
GGCAGCT	460	0.0	21.717392	18
CCGGCAG	460	0.0	21.717392	16
CGGCAGC	475	0.0	21.421053	17
GTATCAA	4960	0.0	21.409275	1
>>END_MODULE