FastQCFastQC Report
Fri 10 Feb 2017
ERR1633211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616830
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29450.4774411101924355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.33963977108765786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11800.1913006825219266No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9090.14736637323087398No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8690.14088160433182564No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8120.13164080865068173No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7900.1280741857562051No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA7480.12126517841220433No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT6990.11332133651087009No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6490.10521537538705965No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6230.1010002756026782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAG250.00549439429.5999987
TGCGTTA502.7173337E-729.59999837
ATGCGTT556.253849E-726.9090936
GGTATCA5900.025.0847471
TAGACTA551.9008066E-523.5454545
AATGCGT652.6795005E-622.7692335
GTATCAA14700.022.149661
CTAGACT450.003823828220.5555554
TGGACCG450.003823828220.5555555
GTCGCCA555.139382E-420.18181812
TGCGATA555.139382E-420.18181810
CGATAAG555.139382E-420.18181812
CGCTCTC656.895504E-519.92307729
CGTTTAG759.254358E-619.73333426
TGCGTTT851.2433939E-619.58823624
ATTACCC1355.638867E-1119.1851863
GCCGGCA609.2302E-418.515
CCGCTCT701.21803474E-418.528
GCCGCTC609.2302E-418.527
GTCAACC1103.842979E-818.515
Produced by FastQC (version 0.11.5)