##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	616830
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96475528103367	33.0	33.0	33.0	33.0	33.0
2	32.07281909115964	33.0	33.0	33.0	33.0	33.0
3	31.776351669017394	33.0	33.0	33.0	33.0	33.0
4	32.05558419661819	33.0	33.0	33.0	33.0	33.0
5	32.15364849310183	33.0	33.0	33.0	33.0	33.0
6	35.36217596420408	37.0	37.0	37.0	33.0	37.0
7	35.50532561645834	37.0	37.0	37.0	33.0	37.0
8	35.624955011915766	37.0	37.0	37.0	33.0	37.0
9	35.7293176401926	37.0	37.0	37.0	33.0	37.0
10	35.73938362271615	37.0	37.0	37.0	33.0	37.0
11	35.75789439553848	37.0	37.0	37.0	33.0	37.0
12	35.7730719971467	37.0	37.0	37.0	33.0	37.0
13	35.76011867127085	37.0	37.0	37.0	33.0	37.0
14	35.73891996173986	37.0	37.0	37.0	33.0	37.0
15	35.75998249112397	37.0	37.0	37.0	33.0	37.0
16	35.73793427686721	37.0	37.0	37.0	33.0	37.0
17	35.73711071121703	37.0	37.0	37.0	33.0	37.0
18	35.71276688877	37.0	37.0	37.0	33.0	37.0
19	35.7069792325276	37.0	37.0	37.0	33.0	37.0
20	35.715260282411684	37.0	37.0	37.0	33.0	37.0
21	35.71333916962534	37.0	37.0	37.0	33.0	37.0
22	35.620138449816	37.0	37.0	37.0	33.0	37.0
23	35.66759885219591	37.0	37.0	37.0	33.0	37.0
24	35.66025323022551	37.0	37.0	37.0	33.0	37.0
25	35.67608092991586	37.0	37.0	37.0	33.0	37.0
26	35.57121735324157	37.0	37.0	37.0	33.0	37.0
27	35.6022258969246	37.0	37.0	37.0	33.0	37.0
28	35.610163254057035	37.0	37.0	37.0	33.0	37.0
29	35.61562991423893	37.0	37.0	37.0	33.0	37.0
30	35.626916654507724	37.0	37.0	37.0	33.0	37.0
31	35.62742084528963	37.0	37.0	37.0	33.0	37.0
32	35.60236045587926	37.0	37.0	37.0	33.0	37.0
33	35.60881766451048	37.0	37.0	37.0	33.0	37.0
34	35.58234845905679	37.0	37.0	37.0	33.0	37.0
35	35.52128301152668	37.0	37.0	37.0	33.0	37.0
36	35.54265680981794	37.0	37.0	37.0	33.0	37.0
37	35.547241541429564	37.0	37.0	37.0	33.0	37.0
38	35.54117828250896	37.0	37.0	37.0	33.0	37.0
39	35.43264594783003	37.0	37.0	37.0	33.0	37.0
40	35.238423876919086	37.0	37.0	37.0	33.0	37.0
41	35.39526125512702	37.0	37.0	37.0	33.0	37.0
42	35.43783052056482	37.0	37.0	37.0	33.0	37.0
43	35.10107809282947	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	14.0
16	36.0
17	26.0
18	20.0
19	18.0
20	26.0
21	71.0
22	244.0
23	519.0
24	1090.0
25	2000.0
26	3402.0
27	5226.0
28	7498.0
29	10378.0
30	13471.0
31	17880.0
32	23072.0
33	30479.0
34	45086.0
35	94382.0
36	361889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.496571178444626	21.7362968727202	14.169057925198192	23.598074023636983
2	15.387221762884426	22.57996530648639	40.03420715594248	21.998605774686705
3	17.32162832547055	28.135142583856165	31.507384530583792	23.035844560089487
4	12.197039702997586	18.319310020589143	40.604866819058735	28.87878345735454
5	12.793800560932509	38.36081254154305	36.017865538316876	12.82752135920756
6	27.76859102183746	41.03448275862069	18.080022048214257	13.116904171327596
7	24.718966327837492	33.476970964447254	24.48292073991213	17.321141967803122
8	23.393965922539437	36.68725580792114	21.331160935752152	18.587617333787268
9	25.168847170208974	16.0074899080784	20.958610962501826	37.8650519592108
10	14.122853946792471	28.87148809234311	34.93798939740285	22.06766856346157
11	32.137866186793765	24.813481834541122	25.220887440623834	17.827764538041276
12	21.71781528135791	26.75113078157677	31.276688877000147	20.254365060065172
13	26.89703808180536	23.26686445211809	27.490718674513236	22.345378791563316
14	21.08846845970527	22.255726861533972	28.96924598349626	27.686558695264495
15	23.12873887456836	29.56081902631195	25.87714605320753	21.433296045912165
16	21.95856232673508	29.882949921372177	25.838237439813238	22.320250312079505
17	21.540943209636367	28.687158536387656	27.21916897686559	22.552729277110387
18	21.58066241914304	28.278618095747614	28.92531167420521	21.21540781090414
19	23.553491237456026	27.374317072775316	29.208047598203716	19.864144091564935
20	23.49123745602516	26.824246550913543	29.292998070781252	20.391517922280045
21	21.622975536209328	28.301476906116758	28.337953731173904	21.737593826500007
22	21.192873238979946	28.34460061929543	28.40717863917125	22.055347502553378
23	21.37396041048587	28.000583629200914	28.96989446038617	21.655561499927046
24	22.368399721154937	28.461488578700777	27.689476841269066	21.480634858875217
25	22.17466725029587	27.637598690076683	28.716826354100807	21.470907705526646
26	21.954185107728225	27.84381434106642	28.484995865959828	21.71700468524553
27	21.86404682003145	27.777669698296126	28.248788158811987	22.109495322860433
28	21.095763824716695	28.005285086652727	29.076893147220467	21.822057941410115
29	22.54575815054391	28.135304703078646	28.062188933741872	21.256748212635575
30	21.691876205761716	28.152489340661123	28.623283562732034	21.532350890845127
31	22.01384498159947	27.875589708671754	28.695102378288993	21.41546293143978
32	20.86425757502067	27.843652221843946	28.835497624953394	22.456592578181994
33	21.22302741436052	27.595123453787917	29.313911450480685	21.86793768137088
34	21.386119352171587	27.850461229187946	28.666893633578134	22.096525785062333
35	21.83113661786878	28.410907381288197	28.191235834832934	21.566720166010082
36	22.28053110257283	28.08277807499635	27.95745991602224	21.679230906408574
37	22.45594410129209	27.183502747920823	27.931682959648523	22.428870191138564
38	22.315873093072646	27.836843214499947	28.191397954055414	21.655885738372
39	21.81184443039411	27.772644002399367	28.55097838950764	21.86453317769888
40	21.87296337726764	27.95373117390529	28.515150041340405	21.658155407486664
41	21.511437511145697	27.782857513415365	28.755897086717574	21.949807888721367
42	21.796118865813916	27.878507854676325	28.40020751260477	21.925165766904982
43	21.838594102102686	27.37334435744046	28.40442261238915	22.3836389280677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	51.5
2	65.0
3	201.0
4	337.0
5	337.0
6	416.5
7	496.0
8	449.0
9	402.0
10	637.5
11	873.0
12	873.0
13	1296.5
14	1720.0
15	3333.5
16	4947.0
17	5650.0
18	6353.0
19	6353.0
20	6441.0
21	6529.0
22	7908.0
23	9287.0
24	11129.5
25	12972.0
26	12972.0
27	15647.5
28	18323.0
29	22488.5
30	26654.0
31	28616.0
32	30578.0
33	30578.0
34	33562.0
35	36546.0
36	38268.0
37	39990.0
38	42654.5
39	45319.0
40	45319.0
41	47520.5
42	49722.0
43	47093.5
44	44465.0
45	44481.5
46	44498.0
47	44498.0
48	43110.0
49	41722.0
50	42161.5
51	42601.0
52	42022.5
53	41444.0
54	41444.0
55	36592.0
56	31740.0
57	27799.5
58	23859.0
59	20837.5
60	17816.0
61	17816.0
62	15487.5
63	13159.0
64	10845.0
65	8531.0
66	7094.5
67	5658.0
68	5658.0
69	4672.5
70	3687.0
71	3200.5
72	2714.0
73	2128.5
74	1543.0
75	1543.0
76	1203.5
77	864.0
78	674.5
79	485.0
80	391.5
81	298.0
82	298.0
83	243.0
84	188.0
85	192.5
86	197.0
87	165.5
88	134.0
89	134.0
90	93.5
91	53.0
92	36.5
93	20.0
94	11.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	616830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.575147930125915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.51052700579022	50.07496158725987
2	6.507124950411537	7.36283113338692
3	1.5672953056335728	2.6600989129923382
4	0.7328942709876591	1.658544071930744
5	0.4428330031093373	1.252667132962633
6	0.3263285713585242	1.1077252319061093
7	0.2332405362513918	0.9236932489207069
8	0.18414527646648887	0.8334437005380433
9	0.13286440839674124	0.6765141203724868
>10	1.0836568795117043	12.817135389923957
>50	0.16118089227692678	6.5147898448242705
>100	0.11268141163712991	11.442409507413423
>500	0.00435624014061065	1.6532196729501336
>1k	8.7124802812213E-4	1.0219664446183494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2945	0.4774411101924355	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2095	0.33963977108765786	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.1913006825219266	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	909	0.14736637323087398	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	869	0.14088160433182564	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	812	0.13164080865068173	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	790	0.1280741857562051	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	748	0.12126517841220433	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	699	0.11332133651087009	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	649	0.10521537538705965	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	623	0.1010002756026782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.6211922247620902E-4	1.6211922247620902E-4	0.0
5	0.0	0.0	1.6211922247620902E-4	3.2423844495241803E-4	0.0
6	0.0	0.0	4.86357667428627E-4	3.2423844495241803E-4	0.0
7	0.0	0.0	0.001459073002285881	4.86357667428627E-4	0.0
8	0.0	0.0	0.002269669114666926	4.86357667428627E-4	0.0
9	0.0	0.0	0.0025939075596193443	0.001134834557333463	0.0
10	0.0	0.0	0.003080265227047971	0.001134834557333463	0.0
11	0.0	0.0	0.004701457451810061	0.001134834557333463	0.0
12	0.0	0.0	0.0050256958967624795	0.0012969537798096721	0.0
13	0.0	0.0	0.0050256958967624795	0.001459073002285881	0.0
14	0.0	0.0	0.0053499343417148975	0.001459073002285881	0.0
15	0.0	0.0	0.005998411231619733	0.00162119222476209	0.0
16	0.0	0.0	0.006646888121524569	0.00162119222476209	0.0
17	0.0	0.0	0.006971126566476987	0.00162119222476209	0.0
18	0.0	0.0	0.006971126566476987	0.001783311447238299	0.0
19	0.0	0.0	0.006971126566476987	0.001945430669714508	0.0
20	0.0	0.0	0.006971126566476987	0.002269669114666926	0.0
21	0.0	0.0	0.006971126566476987	0.002431788337143135	0.0
22	1.6211922247620902E-4	0.0	0.006971126566476987	0.0025939075596193443	0.0
23	1.6211922247620902E-4	0.0	0.006971126566476987	0.00324238444952418	0.0
24	1.6211922247620902E-4	0.0	0.006971126566476987	0.004052980561905225	0.0
25	1.6211922247620902E-4	0.0	0.006971126566476987	0.004539338229333852	0.0
26	1.6211922247620902E-4	0.0	0.006971126566476987	0.0053499343417148975	0.0
27	1.6211922247620902E-4	0.0	0.006971126566476987	0.005836292009143524	0.0
28	3.2423844495241803E-4	0.0	0.006971126566476987	0.015887683802668483	0.0
29	3.2423844495241803E-4	0.0	0.006971126566476987	0.0342071559424801	0.0
30	3.2423844495241803E-4	0.0	0.006971126566476987	0.05706596631162557	0.0
31	3.2423844495241803E-4	0.0	0.006971126566476987	0.11364557495582252	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAG	25	0.005494394	29.599998	7
TGCGTTA	50	2.7173337E-7	29.599998	37
ATGCGTT	55	6.253849E-7	26.90909	36
GGTATCA	590	0.0	25.084747	1
TAGACTA	55	1.9008066E-5	23.545454	5
AATGCGT	65	2.6795005E-6	22.76923	35
GTATCAA	1470	0.0	22.14966	1
CTAGACT	45	0.0038238282	20.555555	4
TGGACCG	45	0.0038238282	20.555555	5
GTCGCCA	55	5.139382E-4	20.181818	12
TGCGATA	55	5.139382E-4	20.181818	10
CGATAAG	55	5.139382E-4	20.181818	12
CGCTCTC	65	6.895504E-5	19.923077	29
CGTTTAG	75	9.254358E-6	19.733334	26
TGCGTTT	85	1.2433939E-6	19.588236	24
ATTACCC	135	5.638867E-11	19.185186	3
GCCGGCA	60	9.2302E-4	18.5	15
CCGCTCT	70	1.21803474E-4	18.5	28
GCCGCTC	60	9.2302E-4	18.5	27
GTCAACC	110	3.842979E-8	18.5	15
>>END_MODULE