FastQCFastQC Report
Fri 10 Feb 2017
ERR1633210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571190
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50510.8842941928255046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37810.6619513646947601No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG21720.38025875803147813No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20300.35539837882315867No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC20050.35102155149775033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19490.3412174582888356No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA18720.32773683012657784No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG15940.27906651026803686No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15650.2739893905705632No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG15280.2675116861289588No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14970.2620844202454525No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT14500.25385598487368477No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14110.24702813424604775No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC14020.24545247640890075No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13500.23634867557205133No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT12680.22199268194471192No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT12570.22006687792153223No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12460.21814107389835258No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12250.21446453894500955No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT12050.21096307708468287No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT11840.20728654213133985No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11840.20728654213133985No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT11710.2050105919221275No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11440.20028361841068648No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG10670.18680299024842872No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10530.18435196694620004No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG10520.1841768938531837No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10430.1826012360160367No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10410.18225108983000404No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG10300.18032528580682433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.18015021271380802No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC10270.17980006652777533No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG10060.1761235315744323No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9940.17402265445823634No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC9940.17402265445823634No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9640.16877046166774629No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9310.16299304959820726No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9270.16229275722614192No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC8920.1561651989705702No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8640.15126315236611285No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT8490.14863705597086785No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA8340.14601095957562282No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8130.1423344246222798No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT8060.14110891297116546No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC8050.14093383987814914No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA8030.14058369369211646No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8020.14040862059910014No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7830.13708223183178977No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7790.13638193945972443No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA7770.13603179327369178No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC7690.1346312085295611No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7690.1346312085295611No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG7620.13340569687844675No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA7410.12972916192510373No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7380.12920394264605473No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7280.12745321171589138No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT7260.1271030655298587No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC7220.12640277315779336No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA7170.1255274076927117No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA7080.12395174985556469No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC7080.12395174985556469No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT7070.12377667676254837No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG6850.119925068716189No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT6840.11974999562317266No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6780.11869955706507467No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6780.11869955706507467No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6750.11817433778602567No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6730.117824191599993No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA6680.11694882613491134No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6400.11204677953045397No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6390.11187170643743763No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6350.1111714140653723No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC6290.1101209755072743No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG6090.10661951364694759No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6070.10626936746091493No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA6070.10626936746091493No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT5910.10346819797265358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTCC200.001841161337.020
ACTAGTG352.3862196E-531.7142878
TTGTTAT303.5972317E-430.83333234
AGCCACG250.005494056729.637
GATTGAT250.005494056729.626
GTGACGG250.005494056729.69
GCTCTCT1100.028.59090830
CGCTCTC1100.028.59090829
CGGGGGG405.9349797E-527.7517
TGCGGTA1200.027.74999836
GCCGCTC1150.027.34782627
CCGGCAG1050.026.42857416
GCCGGCA1050.026.42857415
CCGCTCT1250.025.1628
TTTACCG601.3354402E-624.66666630
GGCAGCT1150.024.13043618
TTCGCCG1400.023.78571524
AGCTTCG1350.023.29629721
CAGCTTC1350.023.29629720
GGCAAGC400.001930004723.12513
Produced by FastQC (version 0.11.5)