FastQCFastQC Report
Fri 10 Feb 2017
ERR1633209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520762
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25000.48006574980509326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.35544068115569105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10020.19241035252188138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGC358.862855E-426.4285728
TAGACTG451.3218552E-424.6666665
AGGACCG451.3218552E-424.6666665
GGTATCA4500.024.6666661
ATAGGGG851.9699655E-923.9411753
TTAGACT551.900069E-523.5454544
CGCCGTC400.001929776923.12526
TTGGCCG855.1670213E-821.76470631
TTCTGCG603.7212492E-521.58333418
GTCGCCA909.453288E-820.55555512
CCGACGA450.003823041520.55555513
CATCCGT450.003823041520.5555559
GTATCAA11400.020.4473691
CGGGTAA656.8928675E-519.92307723
TTAACGG759.249821E-619.73333435
TCGCCAT951.671724E-719.47368413
GGCCGCA951.671724E-719.47368433
ATTAGAC500.007029841718.53
TGGCCGC1002.8674185E-718.532
GTCCGCC500.007029841718.530
Produced by FastQC (version 0.11.5)