##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520762
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53462618240194	33.0	33.0	33.0	27.0	33.0
2	31.752334079675553	33.0	33.0	33.0	27.0	33.0
3	31.476357721953597	33.0	33.0	33.0	27.0	33.0
4	31.775052711219328	33.0	33.0	33.0	27.0	33.0
5	31.870459058072594	33.0	33.0	33.0	33.0	33.0
6	34.92890802324286	37.0	37.0	37.0	27.0	37.0
7	35.08450117328069	37.0	37.0	37.0	33.0	37.0
8	35.217222070734806	37.0	37.0	37.0	33.0	37.0
9	35.33933927590723	37.0	37.0	37.0	33.0	37.0
10	35.3561511784654	37.0	37.0	37.0	33.0	37.0
11	35.35366827840741	37.0	37.0	37.0	33.0	37.0
12	35.376684550716064	37.0	37.0	37.0	33.0	37.0
13	35.373314489152435	37.0	37.0	37.0	33.0	37.0
14	35.35823082329356	37.0	37.0	37.0	33.0	37.0
15	35.36316973972755	37.0	37.0	37.0	33.0	37.0
16	35.34172424255226	37.0	37.0	37.0	33.0	37.0
17	35.34791517046175	37.0	37.0	37.0	33.0	37.0
18	35.32145394633249	37.0	37.0	37.0	33.0	37.0
19	35.30896455578556	37.0	37.0	37.0	33.0	37.0
20	35.31676658435139	37.0	37.0	37.0	33.0	37.0
21	35.30703853199734	37.0	37.0	37.0	33.0	37.0
22	35.21224090851483	37.0	37.0	37.0	33.0	37.0
23	35.2618317004697	37.0	37.0	37.0	33.0	37.0
24	35.25459038869963	37.0	37.0	37.0	33.0	37.0
25	35.267588648941356	37.0	37.0	37.0	33.0	37.0
26	35.14978051393919	37.0	37.0	37.0	33.0	37.0
27	35.168898652359424	37.0	37.0	37.0	33.0	37.0
28	35.19854943333039	37.0	37.0	37.0	33.0	37.0
29	35.18031077536379	37.0	37.0	37.0	33.0	37.0
30	35.18881369992434	37.0	37.0	37.0	33.0	37.0
31	35.17421202007827	37.0	37.0	37.0	33.0	37.0
32	35.168610612909546	37.0	37.0	37.0	33.0	37.0
33	35.15330611680576	37.0	37.0	37.0	33.0	37.0
34	35.118036646299075	37.0	37.0	37.0	33.0	37.0
35	35.062059059608806	37.0	37.0	37.0	33.0	37.0
36	35.0771100809967	37.0	37.0	37.0	33.0	37.0
37	35.07018561262151	37.0	37.0	37.0	33.0	37.0
38	35.065379194334454	37.0	37.0	37.0	33.0	37.0
39	34.96077670797793	37.0	37.0	37.0	27.0	37.0
40	34.74100644824315	37.0	37.0	37.0	27.0	37.0
41	34.92925175031972	37.0	37.0	37.0	27.0	37.0
42	34.963315295662895	37.0	37.0	37.0	27.0	37.0
43	34.57818734853926	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	23.0
17	15.0
18	30.0
19	16.0
20	54.0
21	128.0
22	362.0
23	807.0
24	1686.0
25	2915.0
26	4406.0
27	6421.0
28	9036.0
29	11949.0
30	15106.0
31	19379.0
32	24366.0
33	31476.0
34	45147.0
35	89340.0
36	258086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0899067136235	21.65595800000768	14.293285608396927	23.960849677971897
2	15.492489851410049	22.74801156766431	39.1793179993932	22.580180581532446
3	17.421394034126912	28.0258544210215	31.038363014198424	23.514388530653157
4	12.446376655746771	18.655547063725848	39.94492685718236	28.95314942334502
5	13.188750331245366	38.049051198052084	35.820201934856996	12.941996535845549
6	27.99148171333546	40.74702071195671	18.17356105092153	13.087936523786297
7	25.0963011894109	33.15199649744029	24.317250490627195	17.434451822521613
8	23.68529193758377	36.28298531766911	21.511170169866464	18.520552574880654
9	25.40623163748507	15.873085977855528	20.76726028396849	37.95342210069091
10	14.199768800334894	28.83985390639102	34.80303862416997	22.15733866910412
11	32.36680095705908	24.614123150306668	24.956121990467814	18.06295390216644
12	21.668439709502614	26.64633748238159	31.125158901763182	20.560063906352614
13	26.99812966383876	23.046612464043076	27.412330392770595	22.542927479347572
14	21.324136553742402	22.013318944162595	28.840237958990862	27.82230654310414
15	23.1697013222931	29.487558616028053	25.3716669034991	21.971073158179742
16	21.985667156973822	29.70723670313886	25.775114159635304	22.531981980252016
17	21.508673827967478	28.60807816238512	27.127555389986213	22.75569261966119
18	21.89061413851241	28.15585622606872	28.675863446257598	21.277666189161266
19	23.72484935536771	27.135428468283017	28.886516297272074	20.2532058790772
20	23.569884131330625	26.78939707582351	28.80682538280443	20.83389341004144
21	21.79824948824991	27.952500374451283	28.28931450451454	21.959935632784266
22	21.54189437785399	27.873961617783173	28.355371551687718	22.22877245267512
23	21.58606042683606	27.781788993820594	28.8344771699932	21.797673409350143
24	22.319792918838164	28.1234037813819	27.828067332101803	21.728735967678134
25	22.19401569238923	27.535995329920386	28.535492221014593	21.734496756675796
26	22.105875620725016	27.740887391937203	28.391664522372984	21.7615724649648
27	22.10933209412361	27.588994588698867	27.8428533571958	22.458819959981717
28	21.512130301366074	27.784861414619343	28.892085059969812	21.810923224044764
29	22.585749344230184	27.731286076941096	28.031999262619006	21.650965316209707
30	22.028296995556513	27.917551587865475	28.376686470979067	21.67746494559895
31	22.28926073715056	27.599171982594733	28.47327569983985	21.638291580414855
32	21.284963188558308	27.511223937230444	28.73731186223265	22.4665010119786
33	21.481790145978394	27.46974625644728	29.077774491994425	21.9706891055799
34	21.913657294503057	27.405033393373557	28.553926745807107	22.127382566316285
35	21.890806164812332	28.173330619361625	28.28355371551688	21.652309500309165
36	22.447298381986396	27.755097338131428	28.00127505463148	21.79632922525069
37	22.485895668270725	27.22913730264497	27.992249818535146	22.292717210549156
38	22.538894927049206	27.63930547927844	28.036031814917372	21.785767778754977
39	21.894646690810774	27.54982122351477	28.306788897807444	22.24874318786701
40	21.890422112212487	27.938866507156817	28.48364512003564	21.68706626059505
41	21.711069548085305	27.397352341376674	28.59290808469128	22.29867002584674
42	21.964736290282318	27.807904570609992	28.078085574600298	22.149273564507396
43	21.91519350490243	27.269270799328677	28.36017220918577	22.45536348658312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	27.0
2	35.0
3	159.0
4	283.0
5	283.0
6	336.5
7	390.0
8	398.5
9	407.0
10	537.0
11	667.0
12	667.0
13	1104.0
14	1541.0
15	2842.5
16	4144.0
17	4714.0
18	5284.0
19	5284.0
20	5282.5
21	5281.0
22	6370.0
23	7459.0
24	9076.0
25	10693.0
26	10693.0
27	12848.5
28	15004.0
29	17939.0
30	20874.0
31	22970.0
32	25066.0
33	25066.0
34	27668.5
35	30271.0
36	31908.5
37	33546.0
38	35540.0
39	37534.0
40	37534.0
41	38706.5
42	39879.0
43	38627.0
44	37375.0
45	37517.0
46	37659.0
47	37659.0
48	36564.5
49	35470.0
50	35939.5
51	36409.0
52	36173.0
53	35937.0
54	35937.0
55	32089.0
56	28241.0
57	24805.5
58	21370.0
59	18659.0
60	15948.0
61	15948.0
62	14032.5
63	12117.0
64	9850.5
65	7584.0
66	6390.5
67	5197.0
68	5197.0
69	4276.0
70	3355.0
71	2900.5
72	2446.0
73	1964.0
74	1482.0
75	1482.0
76	1107.0
77	732.0
78	571.0
79	410.0
80	336.5
81	263.0
82	263.0
83	205.5
84	148.0
85	141.0
86	134.0
87	100.0
88	66.0
89	66.0
90	46.5
91	27.0
92	20.5
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	520762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.38000595052879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.47589458585023	53.42175041571996
2	6.4792888336948335	7.824389966673777
3	1.6546656592855016	2.9972616706138266
4	0.7662153865014323	1.850563583853728
5	0.45403450408115903	1.3707303029082887
6	0.30607511565280693	1.1088490382655163
7	0.23551695537323805	0.9954360616820578
8	0.17097836133225186	0.8258939579722423
9	0.13516506523118635	0.734514069866638
>10	1.0901743160219621	13.475464622027431
>50	0.15842515677723834	6.648072358214785
>100	0.07259808572196823	7.704197056435018
>500	0.0	0.0
>1k	9.679744762929094E-4	1.0428768957667633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2500	0.48006574980509326	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1851	0.35544068115569105	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1002	0.19241035252188138	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.760788997661119E-4	0.0	0.0
2	0.0	0.0	5.760788997661119E-4	0.0	0.0
3	0.0	0.0	9.601314996101866E-4	0.0	0.0
4	0.0	0.0	9.601314996101866E-4	0.0	0.0
5	0.0	0.0	0.0011521577995322239	0.0	0.0
6	0.0	0.0	0.0021122892991424103	0.0	0.0
7	0.0	0.0	0.004416604898206858	0.0	0.0
8	0.0	0.0	0.004800657498050933	0.0	0.0
9	0.0	0.0	0.0061448415975051945	3.8405259984407465E-4	0.0
10	1.9202629992203733E-4	0.0	0.007296999397037418	3.8405259984407465E-4	0.0
11	3.8405259984407465E-4	0.0	0.010369420195790015	5.760788997661119E-4	0.0
12	3.8405259984407465E-4	0.0	0.011329551695400201	7.681051996881493E-4	0.0
13	3.8405259984407465E-4	0.0	0.011905630595166314	7.681051996881493E-4	0.0
14	3.8405259984407465E-4	0.0	0.012673735794854464	7.681051996881493E-4	0.0
15	3.8405259984407465E-4	0.0	0.014017919894308725	7.681051996881493E-4	0.0
16	3.8405259984407465E-4	0.0	0.014593998794074836	9.601314996101866E-4	0.0
17	3.8405259984407465E-4	0.0	0.014786025093996874	0.0011521577995322239	0.0
18	3.8405259984407465E-4	0.0	0.01497805139391891	0.0011521577995322239	0.0
19	3.8405259984407465E-4	0.0	0.01497805139391891	0.0011521577995322239	0.0
20	3.8405259984407465E-4	0.0	0.015938182893529097	0.0011521577995322239	0.0
21	3.8405259984407465E-4	0.0	0.01651426179329521	0.0013441840994542612	0.0
22	3.8405259984407465E-4	0.0	0.01651426179329521	0.001728236699298336	0.0
23	3.8405259984407465E-4	0.0	0.016706288093217246	0.0021122892991424103	0.0
24	3.8405259984407465E-4	0.0	0.016898314393139283	0.00288039449883056	0.0
25	3.8405259984407465E-4	0.0	0.016898314393139283	0.00288039449883056	0.0
26	3.8405259984407465E-4	0.0	0.016898314393139283	0.003456473398596672	0.0
27	3.8405259984407465E-4	0.0	0.016898314393139283	0.004416604898206858	0.0
28	3.8405259984407465E-4	0.0	0.016898314393139283	0.01075347279563409	0.0
29	3.8405259984407465E-4	0.0	0.016898314393139283	0.02803583978861745	0.0
30	3.8405259984407465E-4	0.0	0.016898314393139283	0.0499268379797297	0.0
31	3.8405259984407465E-4	0.0	0.016898314393139283	0.10100583375899164	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCGC	35	8.862855E-4	26.428572	8
TAGACTG	45	1.3218552E-4	24.666666	5
AGGACCG	45	1.3218552E-4	24.666666	5
GGTATCA	450	0.0	24.666666	1
ATAGGGG	85	1.9699655E-9	23.941175	3
TTAGACT	55	1.900069E-5	23.545454	4
CGCCGTC	40	0.0019297769	23.125	26
TTGGCCG	85	5.1670213E-8	21.764706	31
TTCTGCG	60	3.7212492E-5	21.583334	18
GTCGCCA	90	9.453288E-8	20.555555	12
CCGACGA	45	0.0038230415	20.555555	13
CATCCGT	45	0.0038230415	20.555555	9
GTATCAA	1140	0.0	20.447369	1
CGGGTAA	65	6.8928675E-5	19.923077	23
TTAACGG	75	9.249821E-6	19.733334	35
TCGCCAT	95	1.671724E-7	19.473684	13
GGCCGCA	95	1.671724E-7	19.473684	33
ATTAGAC	50	0.0070298417	18.5	3
TGGCCGC	100	2.8674185E-7	18.5	32
GTCCGCC	50	0.0070298417	18.5	30
>>END_MODULE