FastQCFastQC Report
Fri 10 Feb 2017
ERR1633208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741415
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35110.4735539475192706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.3415091413041279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12800.1726428518441089No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11850.15982951518380392No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11440.15429954883567232No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11220.15133224981960172No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10100.13622600028324217No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9660.13029140225110095No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9580.12921238442707525No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9500.12813336660304958No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8960.12084999629087623No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7990.10776690517456486No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7650.10318107942245572No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT7610.10264157051044287No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7530.10156255268641719No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7530.10156255268641719No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7510.10129279823041076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAA352.387173E-531.71428726
TCGACGA250.00549510129.59999822
GGTATCA6550.023.4427471
CGGCGTC400.001930543823.12500224
GTCGACG400.001930543823.12500221
GTATCAA18500.023.11
CGTCAAA502.700849E-422.19999927
GACCGTG450.00382454720.5555557
CGCTATC656.897907E-519.92307712
ATCGCTA656.897907E-519.92307710
GGCGTCA701.2184565E-418.525
ATCATCG609.2326E-418.57
TAGGACC2450.018.122454
AACGGCC2250.018.08888837
TAACGGC2300.017.6956536
TTAACGG2200.017.6590935
ATCAACG24850.017.1971823
TAGACCC650.001579218817.0769234
GGACCGT650.001579218817.0769236
TCAACGC25300.017.0375484
Produced by FastQC (version 0.11.5)