##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741415
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887639176439645	33.0	33.0	33.0	33.0	33.0
2	31.977940829360076	33.0	33.0	33.0	33.0	33.0
3	31.708951127236432	33.0	33.0	33.0	27.0	33.0
4	31.9873417721519	33.0	33.0	33.0	33.0	33.0
5	32.09180823155722	33.0	33.0	33.0	33.0	33.0
6	35.262779954546374	37.0	37.0	37.0	33.0	37.0
7	35.43390543757545	37.0	37.0	37.0	33.0	37.0
8	35.565807273928904	37.0	37.0	37.0	33.0	37.0
9	35.67722125934868	37.0	37.0	37.0	33.0	37.0
10	35.68110842105973	37.0	37.0	37.0	33.0	37.0
11	35.70714512115347	37.0	37.0	37.0	33.0	37.0
12	35.69551465778275	37.0	37.0	37.0	33.0	37.0
13	35.693469919006226	37.0	37.0	37.0	33.0	37.0
14	35.668632277469435	37.0	37.0	37.0	33.0	37.0
15	35.694443732592404	37.0	37.0	37.0	33.0	37.0
16	35.67793475988481	37.0	37.0	37.0	33.0	37.0
17	35.67849450038103	37.0	37.0	37.0	33.0	37.0
18	35.650352366758156	37.0	37.0	37.0	33.0	37.0
19	35.65297303129826	37.0	37.0	37.0	33.0	37.0
20	35.65165932709751	37.0	37.0	37.0	33.0	37.0
21	35.65493144864887	37.0	37.0	37.0	33.0	37.0
22	35.5570402541087	37.0	37.0	37.0	33.0	37.0
23	35.61442242199038	37.0	37.0	37.0	33.0	37.0
24	35.59675350512196	37.0	37.0	37.0	33.0	37.0
25	35.61797643694827	37.0	37.0	37.0	33.0	37.0
26	35.51351672140434	37.0	37.0	37.0	33.0	37.0
27	35.5284543744057	37.0	37.0	37.0	33.0	37.0
28	35.55022221023314	37.0	37.0	37.0	33.0	37.0
29	35.556918864603496	37.0	37.0	37.0	33.0	37.0
30	35.55724122117842	37.0	37.0	37.0	33.0	37.0
31	35.54506315626201	37.0	37.0	37.0	33.0	37.0
32	35.54654006190865	37.0	37.0	37.0	33.0	37.0
33	35.54147677076941	37.0	37.0	37.0	33.0	37.0
34	35.524714228873165	37.0	37.0	37.0	33.0	37.0
35	35.46173735357391	37.0	37.0	37.0	33.0	37.0
36	35.4881759878071	37.0	37.0	37.0	33.0	37.0
37	35.48151709906058	37.0	37.0	37.0	33.0	37.0
38	35.464612936074936	37.0	37.0	37.0	33.0	37.0
39	35.3698333591848	37.0	37.0	37.0	33.0	37.0
40	35.16557933141358	37.0	37.0	37.0	33.0	37.0
41	35.334209585724594	37.0	37.0	37.0	33.0	37.0
42	35.37296925473588	37.0	37.0	37.0	33.0	37.0
43	35.056986977603636	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	24.0
16	29.0
17	25.0
18	20.0
19	25.0
20	36.0
21	98.0
22	276.0
23	673.0
24	1443.0
25	2551.0
26	4358.0
27	6659.0
28	9488.0
29	13129.0
30	17315.0
31	22632.0
32	29162.0
33	38447.0
34	55945.0
35	115334.0
36	423745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.239791479805504	20.62448156565486	13.407875481343106	24.727851473196523
2	16.124572607783765	21.488639965471428	38.802964601471515	23.583822825273295
3	17.45513646203543	27.651450267394107	29.843609854130275	25.049803416440184
4	12.215830540250737	17.7688608943709	40.31385930956347	29.701449255814893
5	13.44752938637605	37.64221117727588	35.570227200690574	13.340032235657493
6	29.063614844587715	40.3612012165926	17.48359555714411	13.091588381675578
7	26.440252759925276	32.27086044927604	23.908607190305027	17.38027960049365
8	24.450543892421923	35.74125152579864	20.599933910158278	19.208270671621158
9	25.926100766777044	15.200528718733771	20.488390442599623	38.384980071889565
10	15.169506956293032	27.737097307176146	33.97314594390456	23.120249792626264
11	33.53425544398211	23.91508129724918	24.23865176722888	18.312011491539828
12	23.052541424168652	26.15633619497852	29.768618115360496	21.022504265492337
13	27.356608646979087	22.398791500037092	26.277051314041394	23.967548538942427
14	21.910266180209465	21.020346229844282	28.032883068187182	29.03650452175907
15	24.126164159074204	28.589656265384434	24.85220827741548	22.43197129812588
16	22.711032282864522	28.30452580538565	25.333854858614945	23.650587053134885
17	22.259867955193783	27.62514920793348	26.431620617333074	23.683362219539664
18	22.44910070608229	27.46437555215365	28.0671418841	22.01938185766406
19	24.65865945523088	26.276781559585388	27.54907845133967	21.51548053384407
20	23.754712273153363	26.43526230248916	27.88074155499956	21.929283869357917
21	23.026779873620036	27.187337725835057	27.740873869560232	22.04500853098467
22	22.61513457375424	26.96600419468179	27.54570652063959	22.873154710924382
23	22.623227207434432	26.8740179251836	28.126622741649417	22.37613212573255
24	22.950978871482235	27.56877052662814	26.458191431249706	23.022059170639924
25	22.769973631501923	27.125429078181583	27.81519122219	22.28940606812649
26	23.04957412515258	27.236837668512237	27.83542280639048	21.8781653999447
27	22.95003473088621	27.157664735674352	26.895328527208108	22.996972006231328
28	22.24503146011343	26.77380414477722	28.338919498526465	22.642244896582888
29	24.172831679963313	26.526574185847334	27.063385553300108	22.237208580889245
30	23.29626457517045	26.49123635211049	27.849315160874816	22.363183911844246
31	22.867759621804254	26.72430420210004	27.78538335480129	22.622552821294416
32	21.7605524571259	26.666711625742668	28.090880276228564	23.481855640902868
33	21.824484263199423	26.90139800246825	28.482428869121883	22.791688865210443
34	22.944235010082075	26.63906179400201	28.102479717836836	22.31422347807908
35	22.899455770385007	27.188551620887086	27.403815676780212	22.508176931947695
36	23.772111435565776	27.027103578967242	26.793900851749697	22.406884133717284
37	22.814483116742984	26.51376084918703	27.382235320299696	23.28952071377029
38	23.23894175326909	26.887235893527915	27.08483103255262	22.78899132065038
39	22.26728620273396	26.99459816701847	27.76420763000479	22.97390800024278
40	22.614190433158218	27.35148331231497	27.96544445418558	22.068881800341238
41	22.401893676281166	26.241578603076547	27.95263111752527	23.403896603117012
42	21.97703040807105	27.70202922789531	27.60329909699696	22.717641267036683
43	23.100153085653783	25.973172919350162	27.651315390166104	23.275358604829954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	48.5
2	56.0
3	221.0
4	386.0
5	386.0
6	469.0
7	552.0
8	500.0
9	448.0
10	647.0
11	846.0
12	846.0
13	1349.5
14	1853.0
15	3334.0
16	4815.0
17	5417.0
18	6019.0
19	6019.0
20	5456.5
21	4894.0
22	5383.0
23	5872.0
24	7705.0
25	9538.0
26	9538.0
27	12038.5
28	14539.0
29	17538.5
30	20538.0
31	24321.0
32	28104.0
33	28104.0
34	34815.0
35	41526.0
36	45117.0
37	48708.0
38	51792.0
39	54876.0
40	54876.0
41	57275.5
42	59675.0
43	60851.0
44	62027.0
45	60156.5
46	58286.0
47	58286.0
48	55770.0
49	53254.0
50	53130.5
51	53007.0
52	50447.0
53	47887.0
54	47887.0
55	46573.0
56	45259.0
57	38792.5
58	32326.0
59	29363.0
60	26400.0
61	26400.0
62	23506.5
63	20613.0
64	16490.0
65	12367.0
66	10246.0
67	8125.0
68	8125.0
69	6846.0
70	5567.0
71	4769.5
72	3972.0
73	3997.5
74	4023.0
75	4023.0
76	3386.5
77	2750.0
78	2014.0
79	1278.0
80	859.0
81	440.0
82	440.0
83	331.0
84	222.0
85	196.0
86	170.0
87	136.5
88	103.0
89	103.0
90	72.5
91	42.0
92	26.5
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	741415.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.13137888882371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.30663553122133	44.99283917497714
2	7.6708088816437465	7.997796883854484
3	1.9148777415794553	2.9947565121616093
4	0.9141139599191295	1.9061608476842862
5	0.5542454365500258	1.4446789425095448
6	0.38827209843581817	1.2144695925309776
7	0.2779300597271914	1.0142214073762135
8	0.20653326670818248	0.861349118392859
9	0.1709290016732872	0.8019688094386666
>10	1.2860043589219585	13.412603347785042
>50	0.1705964995111317	6.256233196142877
>100	0.12906446801023463	12.578374385218464
>500	0.00814867313302205	2.909753698027042
>1k	0.0018400229655211083	1.6147940839008512
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3511	0.4735539475192706	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2532	0.3415091413041279	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1280	0.1726428518441089	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1185	0.15982951518380392	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1144	0.15429954883567232	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1122	0.15133224981960172	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1010	0.13622600028324217	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	966	0.13029140225110095	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	958	0.12921238442707525	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	950	0.12813336660304958	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	896	0.12084999629087623	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	799	0.10776690517456486	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	765	0.10318107942245572	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	761	0.10264157051044287	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	753	0.10156255268641719	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	753	0.10156255268641719	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	751	0.10129279823041076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	2.6975445600642016E-4	0.0	0.0
3	0.0	0.0	2.6975445600642016E-4	0.0	0.0
4	0.0	0.0	4.046316840096302E-4	2.6975445600642016E-4	0.0
5	0.0	0.0	4.046316840096302E-4	2.6975445600642016E-4	0.0
6	0.0	0.0	8.092633680192604E-4	2.6975445600642016E-4	0.0
7	0.0	0.0	0.003102176244073832	2.6975445600642016E-4	0.0
8	0.0	0.0	0.003776562384089882	2.6975445600642016E-4	0.0
9	0.0	0.0	0.0043160712961027225	0.0012138950520288907	0.0
10	6.743861400160504E-4	0.0	0.004720702980112353	0.0012138950520288907	0.0
11	6.743861400160504E-4	0.0	0.007148493084170134	0.001483649508035311	0.0
12	6.743861400160504E-4	0.0	0.0076880019961829745	0.0016185267360385208	0.0
13	6.743861400160504E-4	0.0	0.008227510908195814	0.0016185267360385208	0.0
14	6.743861400160504E-4	0.0	0.008901897048211866	0.0016185267360385208	0.0
15	6.743861400160504E-4	0.0	0.009441405960224705	0.001753403964041731	0.0
16	6.743861400160504E-4	1.3487722800321008E-4	0.010655301012253596	0.001888281192044941	0.0
17	6.743861400160504E-4	1.3487722800321008E-4	0.011329687152269646	0.001888281192044941	0.0
18	6.743861400160504E-4	1.3487722800321008E-4	0.011599441608276067	0.001888281192044941	1.3487722800321008E-4
19	6.743861400160504E-4	1.3487722800321008E-4	0.011869196064282488	0.001888281192044941	1.3487722800321008E-4
20	6.743861400160504E-4	1.3487722800321008E-4	0.012138950520288906	0.0021580356480513613	1.3487722800321008E-4
21	6.743861400160504E-4	1.3487722800321008E-4	0.012678459432301748	0.002967299016070622	1.3487722800321008E-4
22	6.743861400160504E-4	1.3487722800321008E-4	0.012678459432301748	0.003776562384089882	1.3487722800321008E-4
23	6.743861400160504E-4	1.3487722800321008E-4	0.012813336660304958	0.0043160712961027225	1.3487722800321008E-4
24	6.743861400160504E-4	1.3487722800321008E-4	0.013083091116311377	0.004990457436118773	1.3487722800321008E-4
25	6.743861400160504E-4	1.3487722800321008E-4	0.013083091116311377	0.005125334664121983	1.3487722800321008E-4
26	6.743861400160504E-4	1.3487722800321008E-4	0.013083091116311377	0.0057997208041380335	1.3487722800321008E-4
27	6.743861400160504E-4	1.3487722800321008E-4	0.013083091116311377	0.006878738628163714	1.3487722800321008E-4
28	8.092633680192604E-4	2.6975445600642016E-4	0.013083091116311377	0.021310602024507193	1.3487722800321008E-4
29	8.092633680192604E-4	2.6975445600642016E-4	0.013217968344314587	0.044374608013056115	1.3487722800321008E-4
30	8.092633680192604E-4	2.6975445600642016E-4	0.013217968344314587	0.07593587936580727	1.3487722800321008E-4
31	8.092633680192604E-4	2.6975445600642016E-4	0.013217968344314587	0.14566740624346688	1.3487722800321008E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAA	35	2.387173E-5	31.714287	26
TCGACGA	25	0.005495101	29.599998	22
GGTATCA	655	0.0	23.442747	1
CGGCGTC	40	0.0019305438	23.125002	24
GTCGACG	40	0.0019305438	23.125002	21
GTATCAA	1850	0.0	23.1	1
CGTCAAA	50	2.700849E-4	22.199999	27
GACCGTG	45	0.003824547	20.555555	7
CGCTATC	65	6.897907E-5	19.923077	12
ATCGCTA	65	6.897907E-5	19.923077	10
GGCGTCA	70	1.2184565E-4	18.5	25
ATCATCG	60	9.2326E-4	18.5	7
TAGGACC	245	0.0	18.12245	4
AACGGCC	225	0.0	18.088888	37
TAACGGC	230	0.0	17.69565	36
TTAACGG	220	0.0	17.65909	35
ATCAACG	2485	0.0	17.197182	3
TAGACCC	65	0.0015792188	17.076923	4
GGACCGT	65	0.0015792188	17.076923	6
TCAACGC	2530	0.0	17.037548	4
>>END_MODULE