FastQCFastQC Report
Fri 10 Feb 2017
ERR1633207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405925
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34310.8452300301779885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24010.5914885754757652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13840.3409496828231816No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA9450.23280162591611753No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9390.23132352035474535No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG7100.17490915809570734No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG6640.16357701545852066No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6480.15963540062819487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.1574182422861366No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG6330.15594013672476445No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT6000.14781055613721747No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC5930.14608609964894992No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC5450.13426125515797252No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA5380.132536798669705No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT5340.13155139496212356No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5320.13105869310833282No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5140.1266243764242163No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC4750.11701669027529715No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4660.1147995319332389No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4340.1069163022725873No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG4200.10346738929605222No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT4170.10272833651536614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA200.001840533637.025
GCTCGGT200.001840533637.026
TCGGTAG200.001840533637.028
CTATCGC250.00549220729.630
TTGTCGG250.00549220729.622
GCCGCTC803.274181E-1127.75000227
GGTATCA4600.027.3478261
CGAGTCG556.244991E-726.9090921
TCCGCCG509.072182E-625.90000231
GAGTCGC601.3338486E-624.66666622
CGCCTGG601.3338486E-624.66666636
GTATCAA13950.024.6666661
GGACCGT451.3211531E-424.6666666
CGCCGCC601.3338486E-624.66666633
TGTCCGG601.3338486E-624.66666614
GTCGCCG652.6757534E-622.7692324
CCGCTCT903.810783E-922.6111128
TTCGCCG1107.4578566E-1121.86363624
TGCTCGC1201.0913936E-1121.58333210
GCCGTCC603.718648E-521.58333227
Produced by FastQC (version 0.11.5)